Quorum sensing system is involved in bacteriocin production and bacterial competition of Streptococcus mutans

doi:10.3877/cma.j.issn.1674-1366.2012.04.002

Abstract

Abstract:

Objective

To study the role of ComCDE quorum sensing system of Streptococcus mutans （S.mutans） in bacteriocin production and bacterial competition.

Methods

Growth competition between S.mutans and Streptococcus sanguis （S.sanguis） was analyzed through the bacterial competition assay. Cellular growth and death of S. mutans with and without CSP peptide was monitored by using the laser confocal microscopy and LIVE/DEAD BacLight^TM staining. Bacteriocin and bacteriocin immunity protein related gene expression was determined by real-time RT-PCR.

Results

Competition assay showed that S.mutans ΔcomC， ΔcomD， and ΔcomE mutant lost the ability of competition， and they could not suppress the normal growth of S.sanguinis， while S.mutans UA159 wild type strain inhibited the normal growth of the adjacent S.sanguinis. In the presence of CSP peptide， the ratio of Dead/live cells of S.mutans UA159 was increased 58.1% （P＜0.05）， and bacteriocin and immunity protein related genes SMU.151， SMU.423， and SMU.1913c， were increased 23.3-fold （P=0.00）， 15.9-fold （P＜0.05），and 19.3-fold （P＜0.05） respectively.

Conclusion

Quorum sensing system of S.mutans is involved in bacteriocin production and bacterial competition.

Key words: Streptococcus mutans, ComCDE system, Bacteriocin

Kai ZHANG, Jun-qi LING, Jia LIU, Li-jun HUO, Jun-ni MAI. Quorum sensing system is involved in bacteriocin production and bacterial competition of Streptococcus mutans[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 6(04): 314-319.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2012.04.002

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2012/V6/I04/314

Figures/Tables 5

References 17

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反应物	体积（μl）
RT primer Mix	1
QuantiTect Reverse Transcriptase	1
RNA Template	14
RT Buffer 5 ×	4
Total volume	20

反应物	体积（μl）
RT primer Mix	1
QuantiTect Reverse Transcriptase	1
RNA Template	14
RT Buffer 5 ×	4
Total volume	20

基因	引物序列
SMU.151	上游5'-AGCGGTAGGAGGAGCTGCATCA-3'
	下游5'-CCAACCGCACCAACTCCAGCA-3'
SMU.423	上游5'-GCACAGCTGGTTCTGCAGGTT-3'
	下游5'-AGCCTCCTAAAGCCGCTCCAGA-3'
SMU.1913c	上游5'-AGCTGGTTTTTCAGGTGGCGT-3'
	下游5'-TCCAAGGACTCCACCAACGG-3'

基因	引物序列
SMU.151	上游5'-AGCGGTAGGAGGAGCTGCATCA-3'
	下游5'-CCAACCGCACCAACTCCAGCA-3'
SMU.423	上游5'-GCACAGCTGGTTCTGCAGGTT-3'
	下游5'-AGCCTCCTAAAGCCGCTCCAGA-3'
SMU.1913c	上游5'-AGCTGGTTTTTCAGGTGGCGT-3'
	下游5'-TCCAAGGACTCCACCAACGG-3'

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