Chemical synthesis and biological activities of competence stimulated peptide of quorum sensing system in Streptococcus mutans UA159

doi:10.3877/cma.j.issn.1674-1366.2012.06.002

Abstract

Abstract:

Objective

To synthesize and analyze the biological activities of the competence stimulated peptide （CSP） in Streptococcus mutans （S.mutans） quorum sensing system.

Methods

The CSP peptide derived from S.mutans UA159 comC gene N-terminal 26-46 was chemically synthesized and purified. The biological activity of CSP， which was used as a quorum-sensing signal in S.mutans biofilm formation CSP， was analyzed by scanning electron microscope （SEM）.

Results

The CSP was accurately synthesized， and SEM confirmed the addition of chemically synthetic CSP induced the adhesion of S.mutans cells and significantly increased the biofilm formation of S.mutans.

Conclusion

The CSP was chemically synthesized with biological activities， which made it possible to study the mechanism of quorum sensing system in S.mutans.

Key words: Streptococcus mutans, ComCDE system, Signaling peptide, Quorum sensing

Kai ZHANG, Li-jun HUO, Jun-qi LING, Jia LIU, Jun-ni MAI. Chemical synthesis and biological activities of competence stimulated peptide of quorum sensing system in Streptococcus mutans UA159[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 06(06): 484-489.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2012.06.002

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2012/V06/I06/484

Figures/Tables 7

References 16

1	Yoshida A， Kuramitsu HK. Multiple Streptococcus mutans Genes Are Involved in Biofilm Formation. Appl Environ Microbiol， 2002，68（12）：6283-6291.
2	Luo P， Morrison DA. Transient association of an alternative sigma factor， ComX， with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae. J Bacteriol， 2003，185（1）：349-358.
3	Senadheera D， Cvitkovitch DG. Quorum sensing and biofilm formation by Streptococcus mutans. Adv Exp Med Biol， 2008，631：178-188.
4	van der Ploeg JR. Regulation of bacteriocin production in Streptococcus mutans by the quorum-sensing system required for development of genetic competence. J Bacteriol， 2005，187（12）：3980-3989.
5	Cvitkovitch DG， Li YH， Ellen RP. Quorum sensing and biofilm formation in Streptococcal infections. J Clin Invest， 2003，112（11）：1626-1632.
6	Zhang K， Ou M， Wang W， et al. Effects of quorum sensing on cell viability in Streptococcus mutans biofilm formation.Biochem Biophys Res Commun， 2009，379（4）：933-938.
7	Lee MS， Morrison DA. Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation. J Bacteriol， 1999，181（16）：5004-5016.
8	Kroes I， Lepp PW， Relman DA. Bacterial diversity within the human subgingival crevice. Proc Natl Acad Sci U S A， 1999，96（25）：14547-14552.
9	Ajdi D， McShan WM，McLaughlin RE，et al. Genome sequence of Streptococcus mutans UA159， a cariogenic dental pathogen.Proc Natl Acad Sci USA， 2002，99（22）：14434-14439.
10	Wen ZT， Burne RA. Functional genomics approach to identifying genes required for biofilm development by Streptococcus mutans.Appl Environ Microbiol， 2002，68（3）：1196-1203.
11	Håvarstein LS， Gaustad P， Nes IF， et al. Identification of the streptococcal competence-pheromone receptor. Mol Microbiol，1996，21（4）：863-869.
12	Davies DG， Parsek MR， Pearson JP， et al. The involvement of cell-to-cell signals in the development of a bacterial biofilm.Science， 1998，280（5361）：295-298.
13	Allan E， Hussain HA， Crawford KR， et al. Genetic variation in comC， the gene encoding competence-stimulating peptide（CSP） in Streptococcus mutans. FEMS Microbiol Lett， 2007，268（1）：47-51.
14	Tamura S， Yonezawa H， Motegi M， et al. Inhibiting effects of Streptococcus salivarius on competence-stimulating peptidedependent biofilm formation by Streptococcus mutans. Oral Microbiol Immunol， 2009，24（2）：152-161.
15	Wang BY， Alvarez P， Hong J， et al. Periodontal pathogens interfere with quorum-sensing-dependent virulence properties in Streptococcus mutans. J Periodontal Res， 2011，46（1）：105-110.
16	LoVetri K， Madhyastha S. Antimicrobial and antibiofilm activity of quorum sensing peptides and Peptide analogues against oral biofilm bacteria. Methods Mol Biol， 2010，618：383-392.

氨基酸	重量（mg）
甘氨酸Fmoc-Gly-OH	64.22
亮氨酸Fmoc-Leu-OH	76.34
丙氨酸Fmoc-Ala-OH	67.25
谷氨酰胺Fmoc-Gln（Trt）-OH	131.92
苏氨酸Fmoc-Thr（tBu）-OH	85.86
苯丙氨酸Fmoc-Phe-OH	83.69
丝氨酸Fmoc-Ser（tBu）-OH	82.83
精氨酸Fmoc-Arg（Pmc）-OH.IPE	165.24
天冬氨酸Fmoc-Asn（Trt）-OH	128.89

氨基酸	重量（mg）
甘氨酸Fmoc-Gly-OH	64.22
亮氨酸Fmoc-Leu-OH	76.34
丙氨酸Fmoc-Ala-OH	67.25
谷氨酰胺Fmoc-Gln（Trt）-OH	131.92
苏氨酸Fmoc-Thr（tBu）-OH	85.86
苯丙氨酸Fmoc-Phe-OH	83.69
丝氨酸Fmoc-Ser（tBu）-OH	82.83
精氨酸Fmoc-Arg（Pmc）-OH.IPE	165.24
天冬氨酸Fmoc-Asn（Trt）-OH	128.89

[1]	Jing Wang, Le Zhao, Jiankang Zeng, Peijie Li, Fei Tan, Jiahuan Li, Yongjie Qiao, Shenghu Zhou. Research progress on treatment of early femoral head necrosis with platelet rich plasma [J]. Chinese Journal of Joint Surgery(Electronic Edition), 2024, 18(06): 773-777.
[2]	Yaguang Han, Yiqin Zhou, Qirong Qian. Analysis of platelet-rich plasma treatment efficacy for tendinopathy: from basic research to clinical study [J]. Chinese Journal of Joint Surgery(Electronic Edition), 2018, 12(04): 551-560.
[3]	Junqiang Li, Chenqi Tang, Zhaofan Xia. Research progress of pseudomonas aeruginosa quorum sensing inhibitors [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2015, 10(05): 450-453.
[4]	Li Zhang, Yang Zhang, Jingjing Ma, Zhehao Yu, Liang Ge, Linchun Sun. Surface-anchored protein SasX involves in the biofilm formation and pathogenicity of Staphylococcus aureus ST239 clone by regulating RNAⅢ [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2023, 17(04): 252-259.
[5]	Xinyi Deng, Zhenyu Zeng, Xiaolan Li. Research progress on bacterial quorum sensing signal on host immune regulation mechanism [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2023, 17(02): 140-147.
[6]	Xiaolan Li, Xiao Wang, Junqi Ling, Xiaoli Hu, Dongmei Deng. Application of green fluorescent protein reporter system in Streptococcus mutans for study on dual-species biofilms [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2019, 13(03): 136-143.
[7]	Fangcao Lei, Yan Yang, Jianying Zhang. Research progress on the regulation and mechanism of Hsp100/Clp ATPase on virulence expressions in Streptococcus mutans [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2018, 12(03): 190-194.
[8]	Ruixue Wu, Xin Yang, Yijing Chen, Yuanxiang Fu, Dongsheng Yu, Wei Zhao. Effect of graphene oxide-silver nanocomposites on Streptococcus mutans proliferation and biofilm formation [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2018, 12(02): 83-88.
[9]	Lixia Yu, Peilin Zhuang, Huancai Lin. The correlation between the sortaseA genotype of Streptococcus mutans and high-severity childhood caries [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2016, 10(04): 238-243.
[10]	Ke Ma, Yan Gao, Zhongchun Tong, Xiaoqiong Jiang, Dongjia Lin. Influence of different finishing and polishing techniques on the surface roughness of nanofilled composite resin and adhesion of Streptococcus mutans [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2016, 10(01): 17-21.
[11]	Huihui Zeng, Junqi Ling, Jia Liu. Effect of an efflux pump inhibitor on cariogenic factors of Streptococcus mutans [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(06): 442-448.
[12]	Huihui Zeng, Junqi Ling. Effects of ATP-binding cassette exporters on virulence factors in Streptococcus mutans [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(03): 247-251.
[13]	Li-jun HUO, Hong-yan LIU, Jun-qi LING, Xiang-ya Huang. The construction and identification of the ldh-luc reporter strain of Streptococcus mutans and the reporter strain [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 06(06): 479-483.
[14]	Hang Dong, Donghao Lang, Xue Fu, Fang Li, Lingfeng Wang. Research status of quorum sensing system and mechanism of biofilm resistance [J]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(01): 57-60.

Please choose a citation manager

Content to export