Abstract:
Objective
To construct the ldh-luc reporter strain of Streptococcus mutans （S.mutans） and identity the reporter strain.
Methods
Approximately 1 100 000 of sequence upstream of the ldh start codon from S.mutans genome were amplified and cloned to the suicide vector pFW5-luc.Confirmed constructs were transformed into S.mutans and integrated via single crossover homologous recombination. The expected integration was confirmed in antibiotic-resistant clones by PCR and sequencing.
Results
The ldh-luc reporter strain of S.mutans was selected and confirmed by PCR and sequencing analysis of multiple clones for similar reporter activity.
Conclusion
An ldh-luc reporter strain of S.mutans was constructed successfully.
Key words:
Streptococcus mutans,
Luciferase,
Homologous recombination,
Ldh-luc reporter strain
Li-jun HUO, Hong-yan LIU, Jun-qi LING, Xiang-ya Huang. The construction and identification of the ldh-luc reporter strain of Streptococcus mutans and the reporter strain[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 06(06): 479-483.