Effect of graphene oxide-silver nanocomposites on Streptococcus mutans proliferation and biofilm formation

doi:10.3877/cma.j.issn.1674-1366.2018.02.003

Abstract

Abstract:

Objective

To investigate the effect of graphene oxide-silver nanocomposites (GO/Ag) on the proliferation and biofilm formation of Streptococcus mutans (S.mutans) .

Methods

Microdilution method was used to determine the minimum inhibitory concentration (MIC) of graphene oxide (GO) , silver-nanoparticles (AgNPs) , and GO/Ag nanocomposites against the S.mutans. For comparison of antibacterial efficiency of GO, AgNPs and GO/Ag, a colony-forming units counting method was applied. The biomass and the metabolic activities of biofilms with incubation of 24 h were tested by Crystal violet biofilm assay and XTT assay. Confocal laser scanning microscopy (CLSM) was performed to observe and calculate the percentage of live bacteria of S.mutans biofilm. The data were analyzed by One-Way ANOVA and LSD-t test.

Results

The MIC of GO/Ag nanocomposites against S.mutans was found to be 0.16 mg/mL. The bacteriostatic rate of 0.64 mg/mL GO/Ag was (82.90 ± 4.87) % and had significantly difference with the control group (t= 30.804, P<0.001) . In the GO and AgNPs groups, no apparent decrease of S.mutans could be observed, even after raising the concentration up to 2.56 mg/mL. In the 0.16 mg/mL GO/Ag group, reduction rate of biofilm biomass and metabolic activity were (25.12 ± 0.01) % and (38.90 ± 3.42) %, the percentage of live bacteria was (42.76 ± 19.48) %. All of these had significant difference with the blank control group (reduction rate of biofilm biomass: t= 7.274, P<0.001; reduction rate of metabolic activity: t= 7.765, P<0.001; percentage of live bacteria: t= 10.412, P<0.001) .

Conclusions

Comparing with AgNPs and GO, GO/Ag nanocomposites exhibited enhanced inhibition effect on the proliferation and biofilm formation of S.mutans.

Key words: Streptococcus mutans, Nanocomposites, silver, Oxides, graphene, Anti-bacterial property

Ruixue Wu, Xin Yang, Yijing Chen, Yuanxiang Fu, Dongsheng Yu, Wei Zhao. Effect of graphene oxide-silver nanocomposites on Streptococcus mutans proliferation and biofilm formation[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2018, 12(02): 83-88.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2018.02.003

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2018/V12/I02/83

Figures/Tables 4

References 14

[1]	Wu SY，An SS，Hulme J. Current applications of graphene oxide in nanomedicine[J]. Int J Nanomedicine，2015（10 Spec Iss）：9-24.
[2]	Bao Q，Zhang D，Qi P. Synthesis and characterization of silver nanoparticle and graphene oxide nanosheet composites as a bactericidal agent for water disinfection[J]. J Colloid Interface Sci，2011，360（2）：463-470.
[3]	Giusti L，Steinborn C，Steinborn M. Use of silver diamine fluoride for the maintenance of dental prostheses in a high caries-risk pa-tient：A medical management approach[J]. J Prosthet Dent，2017[2017-10-25]. published online ahead of print September 6，2009]. URL
[4]	Morones JR，Elechiguerra JL，Camacho A，et al. The bactericidal effect of silver nanoparticles[J]. Nanotechnology，2005，16（10）：2346-2353.
[5]	Tang J，Chen Q，Xu L，et al. Graphene oxide-silver nanocomposite as a highly effective antibacterial agent with species-specific mechanisms[J]. ACS Appl Mater Interfaces，2013，5（9）：3867-3874.
[6]	Peng JM，Lin JC，Chen ZY，et al. Enhanced antimicrobial activities of silver-nanoparticle-decorated reduced graphene nanocomposites against oral pathogens[J]. Mater Sci Eng C Mater Biol Appl，2017（71）：10-16.
[7]	Marcano DC，Kosynkin DV，Berlin JM，et al. Improved synthesis of graphene oxide[J]. ACS Nano，2010，4（8）：4806-4814.
[8]	Cheng L，Weir MD，Zhang K，et al. Dental plaque microcosm biofilm behavior on calcium phosphate nanocomposite with quaternary ammonium[J]. Dent Mater，2012，28（8）：853-862.
[9]	Zhang HZ，Zhang C，Zeng GM，et al. Easily separated silver nanoparticle-decorated magnetic graphene oxide：Synthesis and high antibacterial activity[J]. J Colloid Interface Sci，2016（471）：94-102.
[10]	Shao W，Liu X，Min H，et al. Preparation，characterization，and antibacterial activity of silver nanoparticle-decorated graphene oxide nanocomposite[J]. ACS Appl Mater Interfaces，2015，7（12）：6966-6973.
[11]	de Moraes AC，Lima BA，de Faria AF，et al. Graphene oxide-silver nanocomposite as a promising biocidal agent against methi-cillin-resistant Staphylococcus aureus[J]. Int J Nanomedicine，2015（10）：6847-6861.
[12]	Zhang M，Zhao Y，Yan L，et al. Interfacial Engineering of Bimetallic Ag/Pt Nanoparticles on Reduced Graphene Oxide Matrix for Enhanced Antimicrobial Activity[J]. ACS Appl Mater Interfaces，2016，8（13）：8834-8840.
[13]	Rachid S，Ohlsen K，Witte W，et al. Effect of subinhibitory antibiotic concentrations on polysaccharide intercellular adhesin expression in biofilm-forming Staphylococcus epidermidis[J]. Antimicrob Agents Chemother，2000，44（12）：3357-3363.
[14]	Frank KL，Reichert EJ，Piper KE，et al. In vitro effects of antimi-crobial agents on planktonic and biofilm forms of Staphylococcus lugdunensis clinical isolates[J]. Antimicrob Agents Chemother，2007，51（3）：888-895.

组别	生成量的减少率	活性的减少率
0.08 mg/mL GO/Ag组	2.46 ± 0.54^a	8.95 ± 4.38
0.12 mg/mL GO/Ag组	17.34 ± 5.76^a	30.23 ± 4.55^a
0.16 mg/mL GO/Ag组	25.12 ± 0.01^a	38.90 ± 3.42^a
0.16 mg/mL GO组	4.58 ± 4.96	4.82 ± 1.16
0.16 mg/mL AgNPs组	0.80 ± 0.42	4.67 ± 0.28

组别	生成量的减少率	活性的减少率
0.08 mg/mL GO/Ag组	2.46 ± 0.54^a	8.95 ± 4.38
0.12 mg/mL GO/Ag组	17.34 ± 5.76^a	30.23 ± 4.55^a
0.16 mg/mL GO/Ag组	25.12 ± 0.01^a	38.90 ± 3.42^a
0.16 mg/mL GO组	4.58 ± 4.96	4.82 ± 1.16
0.16 mg/mL AgNPs组	0.80 ± 0.42	4.67 ± 0.28

组别	生物膜活菌比例	t值	P值
0.08 mg/mL GO/Ag组	52.22 ± 2.14	6.775	0.000
0.12 mg/mL GO/Ag组	44.52 ± 1.47	9.735	0.000
0.16 mg/mL GO/Ag组	42.76 ± 19.48	10.412	0.000
0.16 mg/mL GO组	57.56 ± 2.88	4.718	0.000
0.16 mg/mL AgNPs组	64.85 ± 0.59	1.915	0.062

组别	生物膜活菌比例	t值	P值
0.08 mg/mL GO/Ag组	52.22 ± 2.14	6.775	0.000
0.12 mg/mL GO/Ag组	44.52 ± 1.47	9.735	0.000
0.16 mg/mL GO/Ag组	42.76 ± 19.48	10.412	0.000
0.16 mg/mL GO组	57.56 ± 2.88	4.718	0.000
0.16 mg/mL AgNPs组	64.85 ± 0.59	1.915	0.062

Please choose a citation manager

Content to export