Effect of hydroxyapatite coated porous NiTi alloy on hemolysis and cellular adhesion properties

doi:10.3877/cma.j.issn.1674-1366.2012.03.008

Abstract

Abstract:

Objective

To investigate the biocompatibility of the HA coated porous NiTi alloys in vitro. Hemolysis rate， cell attachment， proliferation， and ALP activity of the human fetal osteoblastic 1.19 cell line （hFOB 1.19） cultured on the HA coated porous NiTi alloys were measured.

Methods

A series of biocompatibility tests of porous NiTi alloy disc-shaped samples （10 mm in diameter and 2 mm in thickness） coated with HA were performed， and the untreated porous NiTi alloy and pure titanium samples were used for a comparative study. The hemolysis of all samples was tested by spectrophotometry.Scanning electron microscope （SEM） was used to observe morphology of hFOB 1.19 incubating on the samples， and MTS method and ALP kit were used to test cell attachment， proliferation and ALP activity.Repeated measurements were carried out with analysis of variance on the data.

Results

Hemolysis rates of the untreated NiTi alloy， HA-coated porous NiTi alloy and pure titanium samples are （0.30 ± 0.11）%，（0.51 ± 0.07）% and （0.27 ± 0.06）%， respectively， which are lower than 5% and acceptable according to the YY/T0127.1 standard. The SEM observation revealed that the hFOB 1.19 attached and spread well on the HA-coated porous NiTi alloy. The cell attachment and cell proliferation tests results showed that cells were better attached （P=0.000） and proliferated （P=0.001） on the HA-coated NiTi alloy samples than on the untreated NiTi alloy samples. ALP activity experimental results showed no significant difference between the HA-coated and untreated NiTi sample groups （P=1）， but the samples in both groups exhibited higher ALP activity than the pure titanium samples （P=0.001， P=0.0004），respectively.

Conclusions

The results indicated that the HA-coated NiTi alloy and untreated NiTi alloy samples showed no hemolysis effect； HA coating can enhance the attachment and proliferation of hFOB 1.19 on the porous NiTi alloy， and ALP activity of the HA-coated and untreated porous NiTi alloys was higher than that of pure Ti.

Key words: Porous NiTi alloy, Surface coating, Hydroxyapatite, Hemocompatibility, Biocompatibility

Xin-ying SHI, Tian TIAN, Xue-li MAO, Jing-xian ZHANG, Xin-ping ZHANG, Ke ZHAO. Effect of hydroxyapatite coated porous NiTi alloy on hemolysis and cellular adhesion properties[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 6(03): 266-271.

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Figures/Tables 6

References 20

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