Influence of periodontal interventions on atherosclerosis in animals

doi:10.3877/cma.j.issn.1674-1366.2012.03.007

Abstract

Abstract:

Objective

We established the periodontitis and atherosclerosis （As） model in rats，simulated with periodontal interventions （including no treatment， tooth extractions， systemic antibiotics，scaling and root planning） to explore the relationship between periodontal diseases and atherosclerosis.

Methods

The SD rats were seperated into 4 groups： Control group （A group）， As group （B group），As+periodontitis group （C group）， periodontitis group （D group）. As+periodontitis groups were seperated in to C1 group （no treatment）， C2 group （scaling and root planning）， C3 group （systemic antibiotics treatment）， C4 group （tooth extractions）. The pathological lesion of carotid artery plaque was stained with hematoxylin and eosin. Serum hsCRP levels were evaluated before periodontal interventions and 1 weeks after interventions by ELISA analysis. All animals were scarificed after 14 weeks.

Results

Histologic sections revealed increased foam cells infiltration in group B （atherosclerosis）， group D （periodontitis）and group C3 （systemic antibiotics）； In the group C1 （no treatment） and group C4 （dental extractions），foam cells and inflammatory cells were evident. In group C2 （scaling and root planning）， fibrous cap of atherosclerotic plaque formed and even ruptured. After periodontal interventions， the serum hsCRP of group B and C was significantly higher than control group （P＜0.05）； Compared with group B， the levels of serum hsCRP of group C2 and C3 were significantly increased （P＜0.05）. The serum hsCRP of group C2 was significantly higher than the group C1 （P＜0.05）.

Conclusions

Periodontitis may accelerates the progression of atherosclerosis in rats. In rats with periodontitis associated with systemically cardiovascular diseases or systemic inflammation， direct periodontal interventions may accelerate carotid atherogenic plaque progression， especially scaling and root planing. Therefore， proper time of periodontal basic therapy on patients with cardiovascular diseases should be carefully considered.

Key words: Periodontitis, Atherosclerosis, Periodontal intervention, High sensitivity Creactive protein

Li-li SUN, Xue-xue SHI, Xiu-yun REN, Le CHANG, Mu LIN, Zi-jie YUE. Influence of periodontal interventions on atherosclerosis in animals[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 6(03): 257-265.

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Figures/Tables 8

References 17

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组别	BI		P	PD（mm）		P
组别	干预前	干预后	P	干预前	干预后	P
A组	0.25±0.13	0.50±0.15	0.216	0.23±0.01	0.25±0.01	0.062
B组	1.00±0.21	1.33±0.22	0.264	0.25±0.02	0.28±0.02	0.324
C1组	4.25±0.13	4.42±0.15	0.397	1.71±0.05	1.98±0.05	0.003^a
C2组	4.08±0.08	0.25±0.13	0.000^a	1.84±0.07	0.31±0.05	0.000^a
C3组	4.17±0.11	2.92±0.08	0.000^a	1.69±0.07	1.12±0.13	0.002^a
C4组	4.33±0.14	4.08±0.15	0.264	1.79±0.06	2.06±0.03	0.003^a
D组	4.08±0.15	4.33±0.14	0.244	1.73±0.07	1.89±0.06	0.078

组别	BI		P	PD（mm）		P
组别	干预前	干预后	P	干预前	干预后	P
A组	0.25±0.13	0.50±0.15	0.216	0.23±0.01	0.25±0.01	0.062
B组	1.00±0.21	1.33±0.22	0.264	0.25±0.02	0.28±0.02	0.324
C1组	4.25±0.13	4.42±0.15	0.397	1.71±0.05	1.98±0.05	0.003^a
C2组	4.08±0.08	0.25±0.13	0.000^a	1.84±0.07	0.31±0.05	0.000^a
C3组	4.17±0.11	2.92±0.08	0.000^a	1.69±0.07	1.12±0.13	0.002^a
C4组	4.33±0.14	4.08±0.15	0.264	1.79±0.06	2.06±0.03	0.003^a
D组	4.08±0.15	4.33±0.14	0.244	1.73±0.07	1.89±0.06	0.078

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