Preparation process and biological properties of a new three-dimensional composite bone repair scaffold

doi:10.3877/cma.j.issn.1674-1366.2024.04.002

Abstract

Abstract:

Objective

To explore the process parameters of polylactate-hydroxyacetate copolymer coral hydroxyapatite nano zinc oxide [poly (lactic-co-glycolic acid) /coralline hydroxyapatite/nano zinc oxide, PLGA/CHA/n-ZnO] by 3D printing technology and evaluate its biocompatibility with periodontal ligament stem cells (PDLSCs) .

Methods

PLGA and CHA/n-ZnO powder were mixed at 4∶1 mass ratio. PLGA/CHA/n-ZnO composite bone repair scaffold was prepared by biological extrusion 3D printing technology. Its physicochemical properties were examined by using X-ray diffraction, scanning electron microscopy, electron universal test, mercury press and contact angle test. The adhesion and growth status of PDLSCs on the surface of the scaffold was evaluated in vitro.

Results

Three different forms of PLGA/CHA/n-ZnO 3D composite bone repair scaffolds were successfully prepared. The peaks of calcium carbonate, hydroxyapatite and zinc oxide were detected. The stent had a rough surface and a porous structure with different pore sizes. Its compression strength and elastic modulus were (12.31 ± 4.80) MPa and (31.18 ± 12.30) MPa, respectively. The pore diameter was 5 nm-350 μm. The stent contact angle was (75.73 ± 5.54) °. PDLSCs showed great adhesion and growth onto the surface of the scaffold.

Conclusion

This study laid the experimental foundation for the development of PDLSCs loaded PLGA/CHA/n-ZnO as a new tissue engineering bone substitute.

Key words: Bone repair stent, Periodontal ligament, Stem cells, Coralline hydroxyapatite

Cheng Yang, Zuer Li, Qing Liu, Yuan Zhao, Chongyan Xu, Jun Su, Wenyun Zhang. Preparation process and biological properties of a new three-dimensional composite bone repair scaffold[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2024, 18(04): 219-229.

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References 60

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打印支架	压缩强度（N/mm²）	压缩屈服应变（%）	压缩屈服应力（MPa）	压缩模量（MPa）
样件1	7.97	27.99	3.74	24.57
样件2	10.06	34.93	5.74	20.55
样件3	18.91	32.18	9.23	48.42
平均值	12.31	31.70	6.24	31.18

打印支架	压缩强度（N/mm²）	压缩屈服应变（%）	压缩屈服应力（MPa）	压缩模量（MPa）
样件1	7.97	27.99	3.74	24.57
样件2	10.06	34.93	5.74	20.55
样件3	18.91	32.18	9.23	48.42
平均值	12.31	31.70	6.24	31.18

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