An experimental study of rabbit mandibular defect reconstruction with individualized prosthesis and tissue engineering

doi:10.3877/cma.j.issn.1674-1366.2019.06.002

Chinese Journal of Stomatological Research(Electronic Edition) ›› 2019, Vol. 13 ›› Issue (06): 328-335. doi: 10.3877/cma.j.issn.1674-1366.2019.06.002

Special Issue: ；

• Basic Science Research • Previous Articles Next Articles

An experimental study of rabbit mandibular defect reconstruction with individualized prosthesis and tissue engineering

Junlan Chen¹, Jinan Wu¹^,^†(), Wei Peng², Jueyao Chen¹, Qiaoyi Zheng¹

^1. Hospital of Stomatology, People′s Hospital, Zhongshan 528403, China
^2. The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China

Received:2019-09-02 Online:2019-12-01 Published:2019-12-01
Contact: Jinan Wu
About author:
Corresponding author: Wu Jinan, Email: gdwujn@163.com
Supported by:
National Natural Science Foundation of China(81371111); Science and Technology Plan of Guangdong Province(2008B030301291); Zhongshan Science and Technology Plan(2019B1098)

Abstract

Abstract:

Objective

To evaluate the effect of individualized prosthesis combined with coralline hydroxyapatite (CHA) and recombinant human bone morphogenetic protein-2 (rhBMP-2) on the reconstruction of rabbit mandibular defect with the aid of rapid prototyping (RP) .

Methods

The radial defect models were made in 27 rabbits, which were evenly divided into 3 groups randomly. Individualized prostheses were obtained with RP. The 3 groups were treated with different materials: group A, individualized prostheses with autogenous bone; group B, individualized prostheses with CHA; group C, individualized prostheses with CHA and rhBMP-2. The measurement of bone mineral density (BMD) and the detection of osteocalcin (OC) and collagen Ⅰ (COL-1) with immunohistochemical staining were performed in 4, 12, and 24 weeks after the operation so as to compare the capacity of bone reconstruction among the three groups. Data were analyzed with One-Way ANOVA.

Results

Both group A (0.537 ± 0.010) and group C (0.530 ± 0.010) showed a large number of trabecular and woven bone, whereas the contents of OC and COL-1 in the defect area were similar (t = 0.007, P>0.05) , both of which were significantly higher than group B (0.415 ± 0.009) (t_AB = 0.122, P_AB<0.001; t_AB = 0.121, P_AB<0.001) .

Conclusions

Upon the individualized three-dimensional titanium with RP, CHA combined with rhBMP-2 was found to have an improved osteoinductive ability, leading to a satisfactory recovery in both the contour and function of rabbit mandibles, which provided a reliable basis for individualized functional reconstruction of mandibular defects.

Key words: Mandibular injuries, Mandibular prosthesis, Hydroxyapatites, Recombinant human bone morphogenetic protein-2, Reverse engineering, Rapid prototyping

Junlan Chen, Jinan Wu, Wei Peng, Jueyao Chen, Qiaoyi Zheng. An experimental study of rabbit mandibular defect reconstruction with individualized prosthesis and tissue engineering[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2019, 13(06): 328-335.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2019.06.002

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2019/V13/I06/328

Figures/Tables 8

References 13

[1]	Lee WB, Choi WH, Lee HG，et al. Mandibular reconstruction with a ready-made type and a custom-made type titanium mesh after mandibular resection in patients with oral cancer[J]. Maxillofac Plast Reconstr Surg，2018，40(1): 35. DOI: 10.1186/s40902-018-0175-z.
[2]	王忠东，陈俊兰，陈觉尧，等.个体化钛支架复合珊瑚羟基磷灰石和重组人骨形成蛋白2修复兔下颌骨缺损[J/CD].中华口腔医学研究杂志（电子版），2012，6(4): 336-341. DOI: 10.3877/cma.j.issn.1674-1366.2012.04.006.
[3]	Khodakaram-Tafti A, Mehrabani D, Shaterzadeh-Yazdi H，et al. Tissue Engineering in Maxillary Bone Defects[J]. World J Plast Surg，2018，7(1): 3-11.
[4]	Wu J, Wang Q, Fu X，et al. Influence of Immunogenicity of Allogeneic Bone Marrow Mesenchymal Stem Cells on Bone Tissue Engineering[J]. Cell Transplant，2016，25(2): 229-242. DOI: 10.3727/096368915X687967.
[5]	Kalaignan P, Mohan JS, Jayakumar A. Assessment of Oral Health-Related Quality of Life for Complex Mandibular Defects Rehabilitated with Computer-guided Implant Restoration[J]. J Int Soc Prev Community Dent，2018，8(3): 277-281. DOI: 10.4103/jispcd.JISPCD_74_18.
[6]	Gravvanis A, Apostolou K, Anterriotis D，et al. Single stage aesthetic and functional reconstruction of composite facial gunshot wound with a chimeric functioning muscle and fibular osseous flap. Case report and review of the literature[J]. Microsurgery，2017，37(6): 674-679. DOI: 10.1002/micr.30140.
[7]	Lin X, Hunziker EB, Liu T，et al. Enhanced biocompatibility and improved osteogenesis of coralline hydroxyapatite modified by bone morphogenetic protein 2 incorporated into a biomimetic coating[J]. Mater Sci Eng C Mater Biol Appl，2019，96: 329-336. DOI: 10.1016/j.msec.2018.11.017.
[8]	Senta H, Park H, Bergeron E，et al. Cell responses to bone morphogenetic proteins and peptides derived from them：biomedical applications and limitations[J]. Cytokine Growth Factor Rev，2009，20(3): 213-222. DOI: 10.1016/j.cytogfr.2009.05.006.
[9]	Zhou M, Peng X, Mao C，et al. The Value of SPECT/CT in Monitoring Prefabricated Tissue-Engineered Bone and Orthotopic rhBMP-2 Implants for Mandibular Reconstruction[J]. PLoS One，2015，10(9): e0137167. DOI: 10.1371/journal.pone.0137167.
[10]	Sales PHDH, Cetira Filho EL, Oliveira Neto JQ，et al. Rapid Prototyping as an Auxiliary in Mandibular Reconstructions[J]. J Craniofac Surg，2017，28(8): e744-e745. DOI: 10.1097/SCS.0000000000003892.
[11]	Kratz SRA, Eilenberger C, Schuller P，et al. Characterization of four functional biocompatible pressure-sensitive adhesives for rapid prototyping of cell-based lab-on-a-chip and organ-on-a-chip systems[J]. Sci Rep，2019，9(1): 9287. DOI: 10.1038/s41598-019-45633-x.
[12]	Kresnoadi U, Rahmania PN, Caesar HU，et al. The role of the combination of Moringa oleifera leaf extract and demineralized freeze-dried bovine bone xenograft（xenograft）as tooth extraction socket preservation materials on osteocalcin and transforming growth factor-beta 1 expressions in alveolar bone of Cavia cobaya [J]. J Indian Prosthodont Soc，2019，19(2): 120-125. DOI: 10.4103/jips.jips_251_18.
[13]	程捷瑶，王美娟，马红，等.脂联素通过腺苷酸激活蛋白激酶途径抑制大鼠肝星状细胞氧化应激并调控转化生长因子β1和Ⅰ型胶原表达[J].中华肝脏病杂志，2015，23(1): 69-72. DOI: 10.3760/cma.j.issn.1007-3418.2015.01.016.

组别	例数	4周	12周	24周
A组	9	0.384 ± 0.009	0.494 ± 0.005	0.537 ± 0.010
B组	9	0.300 ± 0.006	0.399 ± 0.010	0.415 ± 0.009
C组	9	0.350 ± 0.010	0.473 ± 0.005	0.530 ± 0.010

组别	例数	4周	12周	24周
A组	9	0.384 ± 0.009	0.494 ± 0.005	0.537 ± 0.010
B组	9	0.300 ± 0.006	0.399 ± 0.010	0.415 ± 0.009
C组	9	0.350 ± 0.010	0.473 ± 0.005	0.530 ± 0.010

组别	例数	4周	12周	24周
A组	9	0.308 ± 0.016	0.475 ± 0.007	0.507 ± 0.013
B组	9	0.215 ± 0.012	0.351 ± 0.010	0.419 ± 0.006
C组	9	0.257 ± 0.008	0.421 ± 0.008	0.505 ± 0.015

组别	例数	4周	12周	24周
A组	9	0.308 ± 0.016	0.475 ± 0.007	0.507 ± 0.013
B组	9	0.215 ± 0.012	0.351 ± 0.010	0.419 ± 0.006
C组	9	0.257 ± 0.008	0.421 ± 0.008	0.505 ± 0.015

Please choose a citation manager

Content to export

An experimental study of rabbit mandibular defect reconstruction with individualized prosthesis and tissue engineering

RichHTML

PDF (PC)

Knowledge