Expression of periapical bone resorption-regulating proteins induced by lipoteichoic acid

doi:10.3877/cma.j.issn.1674-1366.2012.05.004

Abstract

Abstract:

Objective

To explore the effect of the pathogenic bacteria of refractory periapical periodontitis， Enterococcus faecalis lipoteichoic acid on bone resorption， and to investigate the protein expression of receptor activator of nuclear factor-κB ligand （RANKL） as well as osteoprotegerin （OPG）in HPDLFs stimulated by lipoteichoic acid.

Methods

MTT chromatometry was used to determine HPDLFs activity after stimulated by 0.1， 1.0， 10.0 and 20.0 mg/L lipoteichoic acid for 24， 48 and 72 h.In addition， HPDLFs were collected after stimulation with lipoteichoic acid for 24 h. The protein expression of RANKL and OPG in HPDLFs were then evaluated by cell immunofluorescence and western blotting.

Results

Lipoteichoic acid inhibited HPDLFs viability in a dose-and-time-dependent fashion.RANKL and OPG protein level were both significantly up-regulated after stimulation with 10.0 mg/L Lipoteichoic acid for 24 h. The growth rate of RANKL was faster than OPG. Therefore， the ratio of RANKL/OPG was up-regulated by lipoteichoic acid in a dose-dependent fashion. The gray values and the ratio of the lipoteichoic acid high concentration group were statistically higher than those of the control group （P＜0.05）.

Conclusions

Lipoteichoic acid could inhibit HPDLFs proliferation， and upregulate both the protein expression and the ratio of RANKL/OPG. This indicated that lipoteichoic acid might play a crucial role in refractory periapical periodontitis.

Key words: Lipoteichoic acid, Periapical periodontitis, Receptor activator of nuclear factorκB ligand, Osteoprotegerin, Bone resorption

Luo-dan ZHAO, Lu CHENG, Xiao WANG, Zhi-xiang PENG. Expression of periapical bone resorption-regulating proteins induced by lipoteichoic acid[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 6(05): 410-415.

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Figures/Tables 8

References 17

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组别	抑制率
组别	24 h	48 h	72 h
空白对照组	0.00±2.92	0.00±10.60	0.00±3.18
0.1 mg/L组	8.04±4.55^a	11.21±5.53^a	11.49±3.05^a
1.0 mg/L组	9.07±3.33^b	15.30±2.45^bc	13.92±4.74^b
10.0 mg/L组	14.69±1.77^a	26.38±4.32^ac	38.50±3.37^ac
20.0 mg/L组	18.27±6.62^b	26.51±5.56^bc	39.79±3.15^bc

组别	抑制率
组别	24 h	48 h	72 h
空白对照组	0.00±2.92	0.00±10.60	0.00±3.18
0.1 mg/L组	8.04±4.55^a	11.21±5.53^a	11.49±3.05^a
1.0 mg/L组	9.07±3.33^b	15.30±2.45^bc	13.92±4.74^b
10.0 mg/L组	14.69±1.77^a	26.38±4.32^ac	38.50±3.37^ac
20.0 mg/L组	18.27±6.62^b	26.51±5.56^bc	39.79±3.15^bc

组别	RANKL/GAPDH	OPG/GAPDH	RANKL/OPG
空白对照组	0.0658±0.0045	0.6222±0.0558	0.1059±0.0049
0.1 mg/L组	0.0458±0.0017	0.4227±0.0280	0.1085±0.0055
1.0 mg/L组	0.1557±0.0279	0.3623±0.0132	0.4283±0.0640^a
10.0 mg/L组	0.6007±0.0233^a	0.9729±0.1179	0.6237±0.0828^a
20.0 mg/L组	0.8537±0.1623^a	1.4709±0.3522^a	0.6060±0.2104^a

组别	RANKL/GAPDH	OPG/GAPDH	RANKL/OPG
空白对照组	0.0658±0.0045	0.6222±0.0558	0.1059±0.0049
0.1 mg/L组	0.0458±0.0017	0.4227±0.0280	0.1085±0.0055
1.0 mg/L组	0.1557±0.0279	0.3623±0.0132	0.4283±0.0640^a
10.0 mg/L组	0.6007±0.0233^a	0.9729±0.1179	0.6237±0.0828^a
20.0 mg/L组	0.8537±0.1623^a	1.4709±0.3522^a	0.6060±0.2104^a

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