Repair of rabbit periodontal bone defect with Bio-Oss bone combined with platelet-rich fibrin

doi:10.3877/cma.j.issn.1674-1366.2020.05.002

Abstract

Abstract:

Objective

To explore the effect of tissue-engineered bone Bio-Oss bone combined with platelet-rich fibrin (PRF) on repairing periodontal bone defects by examining the expression of bone morphogenetic protein 2 (BMP-2) , osteoprotegerin (OPG) and nuclear factor κB receptor activator ligand (RANKL) .

Methods

A total of 36 three-month-old male New Zealand white rabbits were divided into four groups by random number table method, i.e., Bio-Oss group, PRF group, Bio-Oss/PRF group and blank control group. For each group, there were nine rabbits. Unilateral periodontal was prepared. In the bone defect model, Bio-Oss bone, PRF and Bio-Oss/PRF composite were implanted in the bone defect, respectively, and the one without any material was used as a blank control. The animals were sacrificed at 4, 8 and 12 weeks after the operation. Gross observation, Masson staining and immunohistochemical observation of BMP-2, OPG, RANKL were performed, and the expression of the animals was analyzed by factorial design.

Results

Masson staining results showed that in the Bio-Oss/PRF group, some mature bones were observed at 8 weeks after surgery, and bone plates were formed at 12 weeks, with a high bone maturity. Factorial analysis showed that the expression of BMP-2 in the Bio-Oss group gradually increased at 4, 8 and 12 weeks, and the difference was statistically significant (F = 51.30, P<0.001) ; the expression of OPG first rose then descended, and the difference was statistically significant (F = 167.03, P<0.001) ; the expression of RANKL gradually decreased with time, and the difference was statistically significant (F = 5.39, P = 0.046) . The expression of BMP-2 in the PRF group at 4, 8 and 12 weeks was not statistically significant (F = 0.68, P = 0.544) ; the expression of OPG and RANKL decreased gradually over time, and the difference was statistically significant (F_OPG = 1070.93, P_OPG<0.001; F_RANKL = 2306.15, P_RANKL<0.001) . The expression of BMP-2, OPG and RANKL in the Bio-Oss/PRF group gradually decreased at 4, 8 and 12 weeks, and the difference was statistically significant (F_BMP-2 = 13.29, P_BMP-2<0.001; F_OPG = 237.91, P_OPG<0.01; F_RANKL = 132.48, P_RANKL<0.001) . In the blank control group, the expression of BMP-2 and OPG increased first and then decreased at 4, 8 and 12 weeks, and the difference was statistically significant (F_BMP-2 = 88.33, P_BMP-2<0.001; F_OPG = 30.06, P_OPG<0.001) , while the expression of RANKL gradually decreased over time, and the difference was statistically significant (F = 56.52, P<0.001) .

Conclusion

The application of Bio-Oss bone composite PRF may promote osteogenesis and repair periodontal bone defects.

Key words: Platelet-rich fibrin, Bone morphogenetic protein 2, Osteoprotegerin, Receptor activator of nuclear factor kappaB ligand, Bio-Oss bone, Periodontal bone defect

Yue Zhang, Hongbin Dong, Wei Zhang, Yuan Yuan, Yang Zhou, Tao Guo. Repair of rabbit periodontal bone defect with Bio-Oss bone combined with platelet-rich fibrin[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2020, 14(05): 280-287.

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References 19

[1]	董红宾，张悦，张琴，等. PRF复合组织工程骨修复牙周骨缺损中基因的表达及意义[J].口腔医学，2017，37(11): 969-975. DOI: 10.13591/j.cnki.kqyx.2017.11.003.
[2]	郭建刚，赵然，侯桂英，等.骨组织成分与Masson三色染色反应的关系分析[J].中医正骨，2001，13(11): 5-6. DOI: 10.3969/j.issn.1001-6015.2001.11.002.
[3]	Dohan Ehrenfest DM, Pinto NR, Pereda A，et al. The impact of the centrifuge characteristics and centrifugation protocols on the cells，growth factors，and fibrin architecture of a leukocyte- and platelet-rich fibrin（L-PRF）clot and membrane[J]. Platelets，2018，29(2): 171-184. DOI: 10.1080/09537104.2017.1293812.
[4]	林雨彤，韩筱堃，赵辉，等.富血小板纤维蛋白在口腔医学中的应用进展[J].大连医科大学学报，2019，41(3): 268-271，279. DOI: 10.11724/jdmu.2019.03.16.
[5]	刘超，周正国，孔庆海. Bio-Oss和邻近自体碎骨移植在牙种植术中的应用[J].徐州医学院学报，2005，25(4): 333-335. DOI: 10.3969/j.issn.1000-2065.2005.04.021.
[6]	李勇，程如玉，彭学生，等.富自体浓缩生长因子联合Bio-Oss骨粉在颌骨囊肿中的疗效研究[J].中华全科医学，2018，16(7): 1113-1115. DOI: 10.16766/j.cnki.issn.1674-4152.000307.
[7]	潘凌峰，张景奎，王嘉涵，等.自体牙本质颗粒联合富血小板血浆对引导骨再生治疗牙周骨缺损的效果分析[J].中华全科医学，2020，18(2): 239-242. DOI: 10.16766/j.cnki.issn.1674-4152.001000.
[8]	D′Addazio G, Artese L, Traini T，et al. Immunohistochemical study of osteopontin in oral squamous cell carcinoma allied to fractal dimension[J]. J Biol Regul Homeost Agents，2018，32(4): 1033-1038.
[9]	Gentili C, Torre M, Cancedda R. Tissue engineering approaches in skeletal pediatric disorders[J]. Eur J Pediatr Surg，2014，24(3): 263-269. DOI: 10.1055/s-0034-1382777.
[10]	Holderfield MT, Hughes CC. Crosstalk between vascular endothelial growth factor，notch，and transforming growth factor-beta in vascular morphogenesis[J]. Circ Res，2008，102(6): 637-652. DOI: 10.1161/CIRCRESAHA.107.167171.
[11]	雷利红.改良富血小板纤维蛋白与浓缩生长因子促牙周组织修复再生作用的比较研究[D].杭州：浙江大学，2018.
[12]	Dohan Ehrenfest DM, Del Corso M, Inchingolo F，et al. Selecting a relevant in vitro cell model for testing and comparing the effects of a Choukroun platelet-rich fibrin（PRF）membrane and a platelet-rich plasma（PRF）gel：Tricks and traps[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod，2010，110(4): 411-413. DOI: 10.1016/j.tripleo.2010.05.055.
[13]	Simonet WS, Lacey DL, Dunstan CR，et al. Osteoprotegerin：a novel seereted Protein Involved in the regulation of bone density[J]. Cell，1997，89(2): 309-319. DOI: 10.1016/S0092-8674(00)80209-3.
[14]	Lacey DL, Tan HL, Lu J，et al. Osteoprotegerin ligand modulates murine osteoc lastsurvival in vitro and vivo[J]. Am J Pathol，2000，157(2): 435-448. DOI: 10.1016/S0002-9440(10)64556-7.
[15]	武志贤，曹伟靖，田莹，等.替硝唑片联合超声波龈下刮治及根面平整治疗慢性牙周炎[J].中国临床研究，2019，32(10): 1400-1403. DOI: 10.13429/j.cnki.cjcr.2019.10.024.
[16]	周恬，吴凯，黎敏，等.白细胞介素17和牙周膜成纤维细胞在体外诱导破骨样细胞生成中的作用[J].中国组织工程研究，2019，23(11): 1680-1686. DOI: 10.3969/j.issn.2095-4344.1136.
[17]	高鑫，曾融生.骨保护素在口腔领域的研究进展[J].国际口腔医学杂志，2019，46(3): 316-319. DOI: 10.7518/gjkq.2019021.
[18]	Wohlfahrt JC, Evensen BJ, Zeza B，et al. A novel non-surgical method for mild peri-implantitis-a multicenter consecutive case series[J]. Int J Implant Dent，2017，3(1): 38. DOI: 10.1186/s40729-017-0098-y.
[19]	Khammissa RA, Feller L, Meyerov R，et al. Peri-implant mucositis and peri-implantitis：clinical and histopathological characteristics and treatment[J]. SADJ，2012，67(3): 122，124-126.

组别	样本量	4周	8周	12周	F值	P值
Bio-Oss组	9	10 244^a± 300^b	155 831^b ± 338^b	21 724^c± 223^c	51.30	0.000
PRF组	9	9930 ± 733^b	10 171 ± 338^a	10 698 ± 119^a	0.68	0.544
Bio-Oss/PRF组	9	21 721^c± 884^c	16 021^b ± 291^b	14 123^a ± 117^b	13.29	0.006
空白对照组	9	1046^a ± 59^a	13 030^c ± 190^a	8807^b ± 440^a	88.33	0.000
F值	?	610.56	5.19	49.50	?	?
P值	?	0.000	0.028	0.000	?	?

组别	样本量	4周	8周	12周	F值	P值
Bio-Oss组	9	10 244^a± 300^b	155 831^b ± 338^b	21 724^c± 223^c	51.30	0.000
PRF组	9	9930 ± 733^b	10 171 ± 338^a	10 698 ± 119^a	0.68	0.544
Bio-Oss/PRF组	9	21 721^c± 884^c	16 021^b ± 291^b	14 123^a ± 117^b	13.29	0.006
空白对照组	9	1046^a ± 59^a	13 030^c ± 190^a	8807^b ± 440^a	88.33	0.000
F值	?	610.56	5.19	49.50	?	?
P值	?	0.000	0.028	0.000	?	?

组别	样本量	4周	8周	12周	F值	P值
Bio-Oss组	9	547^a± 17^a	13 937^b ± 576^b	2368^a ± 17^a	167.03	0.000
PRF组	9	27 571^c± 13^b	8954^b ± 59^a	577^a ± 77^a	1070.93	0.000
Bio-Oss/PRF组	9	89 801^c± 737^c	53 487^b ± 174^c	11 057^a ± 123^c	237.91	0.000
空白对照组	9	7525^a± 62^a	15 878^b ± 146^b	5500^a ± 26^b	30.06	0.001
F值	?	354.27	862.66	23.98	?	?
P值	?	0.000	0.000	0.000	?	?

组别	样本量	4周	8周	12周	F值	P值
Bio-Oss组	9	547^a± 17^a	13 937^b ± 576^b	2368^a ± 17^a	167.03	0.000
PRF组	9	27 571^c± 13^b	8954^b ± 59^a	577^a ± 77^a	1070.93	0.000
Bio-Oss/PRF组	9	89 801^c± 737^c	53 487^b ± 174^c	11 057^a ± 123^c	237.91	0.000
空白对照组	9	7525^a± 62^a	15 878^b ± 146^b	5500^a ± 26^b	30.06	0.001
F值	?	354.27	862.66	23.98	?	?
P值	?	0.000	0.000	0.000	?	?

组别	样本量	4周	8周	12周	F值	P值
Bio-Oss组	9	30 250^b± 119^b	22 297^b± 55^c	19 220^a± 48^c	5.39	0.046
PRF组	9	26 550^c± 80^b	6860^b ± 26^b	476^a± 100^a	2306.15	0.000
Bio-Oss/PRF组	9	52 660^b± 642^c	8178^a ± 12^b	6216^a± 22^b	132.48	0.000
空白对照组	9	16 475^c± 26^a	12 814^b± 17^a	1730^a± 89^a	56.52	0.000
F值	?	56.26	17.13	32.49	?	?
P值	?	0.000	0.001	0.000	?	?

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