Regeneration of dental pulp tissue in mature dog teeth with apical periodontitis using vascular endothelial growth factor and bone morphogenetic protein 2

doi:10.3877/cma.j.issn.1674-1366.2016.01.002

Abstract

Abstract:

Objective

To investigate the feasibility of dental pulp regeneration in mature teeth with apical periodontitis on situ using vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP2) .

Methods

Apical periodontitis model was established in 14 mature anterior teeth in 2 dogs (10-12 months) . The disinfected root canals were filled with peptide hydrogel scaffold composited with different cytokines: VEGF group, BMP2 group, VEGF+BMP2 group and a control group (without cytokines) . Eight weeks after the operation, a histological observation was undertaken to evaluate the regeneration tissue in the root canals.

Results

Eight weeks after the operation, newly formed vascularized connective tissue were found in the root canals which filled with VEGF and VEGF+BMP2. No cells and vessels were observed in the root canals in BMP2 group and control group.

Conclusion

VEGF alone or combinated with BMP2 can induce pulp-like tissue regeneration within the root canals of the mature teeth with apical periodontitis.

Key words: Vascular endothelial growth factors, Bone morphogenetic protein 2, Dental pulp, Regeneration, Periapical periodontitis

Jun Zhou, Zhaojing Wang, Wenjin Chen, Wenxia Chen. Regeneration of dental pulp tissue in mature dog teeth with apical periodontitis using vascular endothelial growth factor and bone morphogenetic protein 2[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2016, 10(01): 5-10.

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Figures/Tables 5

References 14

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