The expression of pluripotency markers in the subpopulations of human bone marrow stromal cells

doi:10.3877/cma.j.issn.1674-1366.2012.05.003

Abstract

Abstract:

Objective

The purpose of this study was to investigate theexpression of pluripotency markers Sox2， c-Myc and hTert in the subpopulations of human bone marrow stromal cells with in vitro culture.

Methods

Human BMSCs were culture with limiting dilution method， 3 samples were selected from fast-， slow-growing clones and mixed culture BMSCs respectively. The expression of Sox2， c-Myc and hTert was investigated by immunofluorescent staining and quantitative Realtime PCR analysis. Statistics analysis was applied.

Results

Sox2 showed positive nucleus expression in fastgrowing clones， and cytoplasm expression in other groups. Sox2 mRNA displayed significantly higher expression level compared with other groups （P＜0.05）. c-Myc and hTert showed similar expression pattern which demonstrated a nucleus location in fast-growing clones and mixed cultured BMSCs，albeit cytoplasm staining in slow-growing clones. Whereas the c-Myc and hTert mRNA showed no difference among three groups （P＞0.05）.

Conclusions

BMSCs from fast-growing clones demonstrated higher expression of reprogramming markers compared with slow-growing clones， indicating that colony forming efficiency may be related with the pluripotency and reprogramming capability of BMSCs. c-Myc and hTert may interact cooperatively during this process.

Key words: Bone marrow stromal cells, Subpopulations, Pluripotency markers

Lu LIU, Xi WEI, Jun-qi LING, Zheng-jun PENG, Li-ping WU. The expression of pluripotency markers in the subpopulations of human bone marrow stromal cells[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 6(05): 405-409.

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Figures/Tables 5

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基因	引物序列
Sox2	Forward：5'-CAC TGF CCC TCT CAC ACA TG-3'
	Reverse：5'-CCC ATT TCC CTC GTT TTT CTT-3'
c-Myc	Forward：5'-CGT CTC CAC ACA TCA GCA CAA-3'
	Reverse：5'-TCT TGG CAG CAG GAT AGT CCT T-3'
hTert	Forward：5'-CGT ACA GGT TTC ACG CAT GTG-3'
	Reverse：5'-CAG GCC GTG TCA GAG ATG AC-3'
18s	Forward：5'-TCG GAA CTG AGG CCA TGA TTA AG-3'
	Reverse：5'-TCT TCG AAC CTC CGA CTT TCG-3'

基因	引物序列
Sox2	Forward：5'-CAC TGF CCC TCT CAC ACA TG-3'
	Reverse：5'-CCC ATT TCC CTC GTT TTT CTT-3'
c-Myc	Forward：5'-CGT CTC CAC ACA TCA GCA CAA-3'
	Reverse：5'-TCT TGG CAG CAG GAT AGT CCT T-3'
hTert	Forward：5'-CGT ACA GGT TTC ACG CAT GTG-3'
	Reverse：5'-CAG GCC GTG TCA GAG ATG AC-3'
18s	Forward：5'-TCG GAA CTG AGG CCA TGA TTA AG-3'
	Reverse：5'-TCT TCG AAC CTC CGA CTT TCG-3'

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