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中华口腔医学研究杂志(电子版) ›› 2019, Vol. 13 ›› Issue (06) : 343 -348. doi: 10.3877/cma.j.issn.1674-1366.2019.06.004

所属专题: 文献

临床研究

牙龈沟液中骨膜蛋白含量与牙周炎的关系
徐惠霞1, 王薇1, 袁理1,(), 何飞1, 朱丽红1, 吴勇1   
  1. 1. 暨南大学第二临床医学院 深圳市人民医院口腔医学中心,深圳 518020
  • 收稿日期:2019-06-06 出版日期:2019-12-01
  • 通信作者: 袁理

Relationship between the content of periostin in gingival crevicular fluid and periodontitis

Huixia Xu1, Wei Wang1, Li Yuan1,(), Fei He1, Lihong Zhu1, Yong Wu1   

  1. 1. Department of Stomatology, Second Clinical Medical College of Jinan University, Shenzhen People′s Hospital, Shenzhen 518020, China
  • Received:2019-06-06 Published:2019-12-01
  • Corresponding author: Li Yuan
  • About author:
    Corresponding author: Yuan Li, Email:
  • Supported by:
    Shenzhen Municipal Health Commission Research Program(201601008)
引用本文:

徐惠霞, 王薇, 袁理, 何飞, 朱丽红, 吴勇. 牙龈沟液中骨膜蛋白含量与牙周炎的关系[J]. 中华口腔医学研究杂志(电子版), 2019, 13(06): 343-348.

Huixia Xu, Wei Wang, Li Yuan, Fei He, Lihong Zhu, Yong Wu. Relationship between the content of periostin in gingival crevicular fluid and periodontitis[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2019, 13(06): 343-348.

目的

探讨骨膜蛋白与牙周炎的关系,检测和对比牙周炎患者与健康对照组龈沟液中骨膜蛋白的含量。

方法

从2016年9月至2018年6月于深圳市人民医院口腔内科的初诊患者中直接抽选法随机选取48例慢性牙周炎患者作为试验组,17名牙周健康者作为对照组。在入选病例选取观察牙,记录观察牙的出血指数(BI)、牙周袋探诊深度(PD)、附着丧失(AL)。依据观察牙PD和AL分为轻度牙周炎组15例、中度牙周炎组22例及重度牙周炎组11例。采集观察牙的龈沟液,酶联免疫吸附实验(ELISA)法检测样本中骨膜蛋白的含量。采用SPSS 25.0统计软件对实验数据进行分析。

结果

牙周病患者龈沟液中均可检测出较低浓度的骨膜蛋白[(0.45 ± 0.13)pg/μL],而牙周健康人群的龈沟液中均检测出较高浓度的骨膜蛋白[(0.56 ± 0.23)pg/μL],两者差异具有统计学意义(t = 2.40,P = 0.019);牙周炎轻度、中度、重度各组骨膜蛋白的浓度值分别为(0.57 ± 0.09)、(0.43 ± 0.09)和(0.34 ± 0.11)pg/μL,骨膜蛋白浓度值随炎症程度加重而逐渐降低;经相关分析,龈沟液中骨膜蛋白与BI呈负相关关系(r = -0.529,P<0.001),与PD、AL均分别呈负相关关系(r = -0.806/-0.772,P<0.001)。

结论

牙周炎患者龈沟液中骨膜蛋白的含量显著低于牙周健康人群;骨膜蛋白可能与牙周炎的病变严重程度之间具有密切关系。

Objective

To investigate the relationship between periostin and periodontitis by detecting and comparing the level of periostin in gingival crevicular fluid (GCF) from patients with and without chronic periodontitis.

Methods

A total of 48 patients with chronic periodontitis and 17 periodontal healthy adults in Shenzhen People′s Hospital were enrolled via direct selection method from September 2016 to June 2018. Periodontal index including bleeding index (BI) , probing depth (PD) and attachment loss (AL) were recorded. The chronic periodontitis patients were divided into mild (15 cases) /moderate (22 cases) and severe periodontitis group (11 cases) . GCF were collected and assays for periostin were performed by enzyme-linked immunosorbent assay (ELISA) . A software (SPSS 25.0) was used for statistical analysis, and the significant level was set at 0.05.

Results

There was a significantly lower concentration of periostin in GCF of periodontitis group [ (0.45 ± 0.13) pg/μL] than that of control group [ (0.56 ± 0.23) pg/μL, t = 2.40, P = 0.019]. The GCF periostin levels of the mild/moderate/severe periodontitis groups were (0.57 ± 0.09) pg/μL, (0.43 ± 0.09) pg/μL and (0.34 ± 0.11) pg/μL, respectively, which decreased gradually as the aggravation of inflammation. There was a negative relationship between the periostin in GCF and all the three clinical parameters, i.e. BI, PD and Al (P<0.001) .

Conclusions

The periostin concentration in GCF of periodontitis patients was significantly lower than that of periodontal healthy people. Periostin may be associated with the severity of periodontitis.

表1 入选研究对象的牙周临床指标情况及比较( ± s
表2 17例牙周健康牙与48例慢性牙周炎牙龈沟液中的骨膜蛋白浓度及龈沟液总蛋白量( ± s
图1 48例慢性牙周炎牙龈沟液中骨膜蛋白浓度(pg/μL)与出血指数(BI)的相关关系散点图
图2 48例慢性牙周炎牙龈沟液中骨膜蛋白浓度(pg/μL)与探诊深度(PD)的相关关系散点图
图3 48例慢性牙周炎牙龈沟液中骨膜蛋白浓度(pg/μL)与附着丧失(AL)的相关关系散点图
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