目的

探讨金银花甘草人工唾液对龋病发生发展的抑制效果。

方法

乙醇提取金银花和甘草有效成分并加入到人工唾液中，采用液体稀释法检测其抑制变形链球菌和远缘链球菌的效果；诱导摘除腮腺后的SD 大鼠致龋，建立人工龋大鼠模型，分别用金银花甘草人工唾液、甲硝唑洗必泰溶液、人工唾液和蒸馏水给4 组大鼠施药，采用Keyes 记分法观察金银花甘草人工唾液对大鼠口腔内龋齿发生发展的抑制效果。

结果

其对变形链球菌ATCC10449 株和远缘链球菌OMZ65 株的最小抑菌浓度分别为25、12.5 mg/ml，最小杀菌浓度分别为200、100 mg/ml。在人工龋大鼠模型中，与蒸馏水及人工唾液组相比，金银花甘草人工唾液龋齿计分明显降低，但仍高于甲硝唑洗必泰组。

结论

金银花甘草人工唾液对变形链球菌和远缘链球菌生长有明显抑制作用，并可有效抑制大鼠口腔内龋齿的发生发展。

Objective

To investigate the anticarious effect of artificial saliva with honeysuckle flower and glycyrrhizia.

Method

The efficient substance extracted from honeysuckle flower and glycyrrhizia was put into artificial saliva， and the antibacterial effect of the artificial saliva on Streptococcus mutans ATCC 10449 and Streptococcus sobrinus OMZ65 were investigated.Then 30 SD rats were fed with cariogenic forage 2000^# and infected with Streptococcus mutans.The rats were divided into 4 groups， with each group fed with artificial saliva with honeysuckle flower and glycyrrhizia， arilin chlorhexidine solutions， artificial saliva or distilled water respectively.The effect of artificial saliva with honeysuckle flower and glycyrrhizia on dental caries development in SD rats was evaluated with Keyes scoring method.

Result

The MIC of the Streptococcus mutans ATCC 10449 and Streptococcus sobrinus OMZ65 were 25 mg/ml and 12.5 mg/ml， respectively.The MBC of the Streptococcus mutans ATCC 10449 and Streptococcus sobrinus OMZ65 were 200 mg/ml and 100 mg/ml， respectively.According to the Keyes scoring，at E，Ds，Dm and Dx levels， the group of artificial saliva with honeysuckle flower and glycyrrhizia was lower than groups of artificial saliva and distilled water， but higher than the group of arilin chlorhexidine solutions.

Conclusion

Artificial saliva with honeysuckle flower and glycyrrhizia inhibit the growth of Streptococcus mutans and Streptococcus sobrinus， and thus exhibit anticarious effect in SD rats.