Experimental study of the treatment for bone defect using bone marrow stromal cells

doi:10.3877/cma.j.issn.1674-1366.2010.03.006

Abstract

Abstract:

Objective

To study the effect of bone marrow stromal cells （BMSC） on the repair of bone defect.

Methods

Ten New Zealand rabbit were used as the experimental animal.Bone marrow was obtained by percutaneous aspiration and cultured in vitro for 2 weeks.Bone defect of 1.5 cm long was surgically formed in both sides of the radii.BMSCs were injected into the defect of the experimental side， while culture medium was injected in the control side.X-ray examination was taken by the end of the 1， 2， 3， and 4 week after injection.At the end of the 4 week， the callus formed in bone defect was separated by surgical operation and histologically examined.

Results

The bone callus formation of the experimental sides was faster than that of the control sides.At the end of the 4 week， all of the bone defects in experimental side were repaired completely with bone trabecula.However， bone trabecula was not formed in the defect of the control side.

Conclusion

Bone marrow stromal cells can promote the bone defects healing.

Key words: Bone marrow stromal cells, Cells culture, Bone tissue engineering, Bone defect

Zhi-ling WU, Dong-lin ZENG, Qian XIE, Rong-sheng ZENG, Min-yi CUI, Miao WANG. Experimental study of the treatment for bone defect using bone marrow stromal cells[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2010, 4(03): 244-249.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2010.03.006

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2010/V4/I03/244

Figures/Tables 7

References 14

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评定指标	评分
骨形成
无骨形成	0
骨形成占缺损25%	1
骨形成占缺损50%	2
骨形成占缺损75%	3
骨形成充满	4
骨塑形
未见塑形	0
骨髓腔形成	2
皮质骨塑形	4
骨折线
清楚	0
部分存在	1
消失	2

评定指标	评分
骨形成
无骨形成	0
骨形成占缺损25%	1
骨形成占缺损50%	2
骨形成占缺损75%	3
骨形成充满	4
骨塑形
未见塑形	0
骨髓腔形成	2
皮质骨塑形	4
骨折线
清楚	0
部分存在	1
消失	2

编号	第2 周					第3 周					第4 周
编号	C	E	D	秩号	C	E	D	秩号	C	E	D	秩号
1	0	1	1	1	3	2	1	1	3	2	1	1
2	0	2	2	5	6	1	5	10	6	2	4	4
3	1	2	1	2	3	2	1	2	6	3	3	3
4	0	2	2	6	6	2	4	7	6	2	4	5
5	1	3	2	7	3	1	2	4	6	2	5	10
6	0	1	1	3	3	2	1	3	3	2	1	2
7	0	2	2	8	3	1	2	5	6	2	4	6
8	1	2	1	4	6	2	4	8	6	2	4	7
9	0	2	2	9	3	1	2	6	6	2	4	8
10	1	3	2	10	6	2	4	9	6	2	4	9
秩和				55				55				55
P				0.001				0.001				0.001

编号	第2 周					第3 周					第4 周
编号	C	E	D	秩号	C	E	D	秩号	C	E	D	秩号
1	0	1	1	1	3	2	1	1	3	2	1	1
2	0	2	2	5	6	1	5	10	6	2	4	4
3	1	2	1	2	3	2	1	2	6	3	3	3
4	0	2	2	6	6	2	4	7	6	2	4	5
5	1	3	2	7	3	1	2	4	6	2	5	10
6	0	1	1	3	3	2	1	3	3	2	1	2
7	0	2	2	8	3	1	2	5	6	2	4	6
8	1	2	1	4	6	2	4	8	6	2	4	7
9	0	2	2	9	3	1	2	6	6	2	4	8
10	1	3	2	10	6	2	4	9	6	2	4	9
秩和				55				55				55
P				0.001				0.001				0.001

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