Expression of histone demethylase KDM5A in human dental pulp cells during odontogenic differentiation process

doi:10.3877/cma.j.issn.1674-1366.2014.06.005

Abstract

Abstract:

Objective

To investigate the expression of KDM5A in human dental pulp cells during in vitro odontogenic differentiation.

Methods

Human dental pulp cells （hDPCs） were cultured from human normal tooth pulp. KDM5A mRNA and protein in hDPCs from passage 1 to passage 8 （P1-P8） were detected using real-time quantitative PCR and western blot. Celluar KDM5A localization in hDPCs was determined by immunofluorescence. HDPCs at passage 3 were cultured in the odontoblastic differentiation induction media for 7 d and 14 d， and the mRNA and protein level of KDM5A were confirmed by real-time quantitative PCR and western blot.

Results

Real-time quantitative PCR and western blot showed that mRNA and protein expression of KDM5A increased at passage 1-2 and decreased at passage 7-8 in hDPCs. KDM5A existed in both the cytoplasm and the nucleus of the hDPCs.KDM5A mRNA and protein expression gradually increased during the odontogenic differentiation of the hDPCs （P ＜0.05）.

Conclusion

KDM5A present in hDPCs. Odontogenic differentiation induction enhance the expression level of KDM5A.

Key words: Histone demethylases, KDM5A, Dental pulp cell, Odontogenic differentiation

Jinling Li, Zhangsong Liao, Lijia Rao, Qiong Xu. Expression of histone demethylase KDM5A in human dental pulp cells during odontogenic differentiation process[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2014, 8(06): 464-470.

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https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2014/V8/I06/464

Figures/Tables 6

References 33

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基因	引物序列
KDM5A	F：5'-TTACCAACAGGTCAGACGCAT-3'
	R：5'-GGTTTGCTACATTCCTCGGCG-3'
β-actin	F：5'-CTGCGGGTATCCATGAGA-3'
	R：5'-TACCACCACTGAGAACGATG-3'

基因	引物序列
KDM5A	F：5'-TTACCAACAGGTCAGACGCAT-3'
	R：5'-GGTTTGCTACATTCCTCGGCG-3'
β-actin	F：5'-CTGCGGGTATCCATGAGA-3'
	R：5'-TACCACCACTGAGAACGATG-3'

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