Abstract:
Objective
To analyze the expression of long non-coding RNA （lncRNA） in the jaw development process of mice.
Methods
lncRNA-seq sequencing technology was used to inspect the difference of lncRNA expression profile between C57 mice of embryonic day 18 and 2 weeks after birth.The lncRNAs with different expression levels were screened out after pretreatment and homogenization of raw data. The hierarchical clustering and volcano scatter diagram analysis were performed.
Results
Mandibular tissues of the C57 mice's of embryonic day 18 and 2 weeks after birth were compared. 6617 lncRNAs showed more than 2 times difference （FDR≤0.001） between the two groups were recognized as lncRNAs with differential expression， accounting for 17.16% of all lncRNAs. Among thses lncRNAs，3720 increased more than 2 times and 2897 reduced more than 2 times， 714 increased more than 5 times and 288 reduced more than 5 times， 645 increased more than 10 times and 211 reduced more than 10 times.
Conclusion
In the jaw development process of mice， the lncRNA expression changes significantly， suggesting that lncRNAs may participate in the regulation of the jaw development.
Key words:
Jaw development,
Long non-coding RNA,
Gene sequencing
Xin Xia, Hong Wu, Jiangyuan Chen, Xiangbo Kong, Yi Ruan. Study on expression profile of long non-coding RNA in the jaw development process of mice[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2014, 8(06): 459-463.