Effect of PI3K/ATK signal pathway on proliferation and odontoblast differentiation of human dental pulp cells in vitro

doi:10.3877/cma.j.issn.1674-1366.2014.03.003

Abstract

Abstract:

Objective

To investigate the effect of PI3K/AKT signaling pathway on proliferation and odontoblast differentiation of human dental pulp cells （hDPCs） in vitro.

Methods

The hDPCs were isolated from extracted third molars of healthy donors and cultured. Cells from the third passage were treated with LY294002. CCK8 assay was used to detect cell proliferation. Western blot analysis was used to detect relative protein level of AKT， p-AKT and DSPP. Mineralization nodules were observed utilizing alizarin red staining after odontongenic differentiation induction for 14 d.

Results

CCK8 assay showed that the proliferation of hDPCs treated with LY294002 was inhibited from 24 h， 48 h and 72 h （24 h：Z=-0.358，P＜0.05；48 h：t=11.674，P＜0.05；72 h：t=10.832，P＜0.05）. Although there is no effect of the total AKT， LY294002 decreased the protein level of p-AKT and DSPP. Mineralization nodules were less observed in LY294002 group after odontongenic differentiation induction for 14 d.

Conclusion

The PI3K/AKT signal pathway can promote the proliferation and odontoblast differentiation of hDPCs.

Key words: PI3K/ATK signal pathway, Human dental pulp cell, Proliferation, Odontoblast differentiation

Xiangyan Guan, Qimeng Li, Huijuan Liu, Qiong Xu. Effect of PI3K/ATK signal pathway on proliferation and odontoblast differentiation of human dental pulp cells in vitro[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2014, 8(03): 191-196.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2014.03.003

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2014/V8/I03/191

Figures/Tables 6

References 13

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蛋白	0 h	6 h	12 h	24 h	48 h	72 h
p-AKT	0.94±0.10	0.43±0.01^a	0.40±0.14^a	0.30±0.12^a	0.42±0.14^a	0.50±0.10^a
AKT	1.44±0.13	1.44±0.12	1.40±0.20	1.35±0.02	1.37±0.12	1.39±0.12
DSPP	0.92±0.02	0.82±0.10	0.56±0.10^b	0.47±0.29^b	0.38±0.15^b	0.31±0.13^b

蛋白	0 h	6 h	12 h	24 h	48 h	72 h
p-AKT	0.94±0.10	0.43±0.01^a	0.40±0.14^a	0.30±0.12^a	0.42±0.14^a	0.50±0.10^a
AKT	1.44±0.13	1.44±0.12	1.40±0.20	1.35±0.02	1.37±0.12	1.39±0.12
DSPP	0.92±0.02	0.82±0.10	0.56±0.10^b	0.47±0.29^b	0.38±0.15^b	0.31±0.13^b

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