The effects of gelsolin on the proliferation and migration of human dental pulp cells

doi:10.3877/cma.j.issn.1674-1366.2016.02.001

Abstract

Abstract:

Objective

To investigate the effects of gelsolin on the proliferation and migration of human dental pulp cells (hDPCs) .

Methods

The expression of gelsolin in hDPCs was examined by laser confocal microscope. Small interfering RNA were transfected into hDPCs to knock down gelsolin mRNA. The proliferation and migratory abilities of hDPCs after gelsolin siRNA transfection were measured respectively by CCK-8 assay and Transwell assay. Cell cycle and apoptosis analysis were conducted by flow cytometry. Data were analyzed using One-Way ANOVA procedure.

Results

Confocal images indicated that gelsolin was widely expressed in the cytoplasm of hDPCs. Silencing of GSN in hDPCs significantly hindered cell proliferation by approximately 47.8% (F = 6.182, P = 0.035) , 48.9% (F= 5.520, P= 0.044) , 64.1% (F= 15.440, P= 0.004) followed by 24, 48, and 72 h culture in growth medium, which might be resulted from cell apoptosis induction instead of cell cycle arrest. The number of cells transferred through the Transwell membrane in si-GSN hDPCs was dramatically reduced by almost 60% (F= 12.781, P<0.001) when compared with controls.

Conclusion

Gelsolin plays a regulatory role in the proliferation and migration of human dental pulp cells.

Key words: Human dental pulp cells, Gelsolin, Proliferation, Migration, Apoptosis

Minhua Zhang, Xuyan Li, Hongwei Jiang, Junqi Ling. The effects of gelsolin on the proliferation and migration of human dental pulp cells[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2016, 10(02): 79-85.

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https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2016/V10/I02/79

Figures/Tables 7

References 14

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