The role of p38β in odontoblastic differentiation of human dental pulp cells

doi:10.3877/cma.j.issn.1674-1366.2015.01.001

Abstract

Abstract:

Objective

To investigate the role of p38β MAPK in odontoblastic differentiation of human dental pulp cells (hDPCs) .

Methods

HDPCs obtained from the healthy third molars or premolars were cultured. Western blot were used to detect the expression of p38β in hDPCs after OM induced for 0, 3, 7 and 14 days. Lentivirus shRNA were constructed and transfected into hDPCs, The protein and mRNA levels of p38β were detected by western blot and quantitative real-time PCR. Then hDPCs were treated with odontogenic medium for 1, 7 and 14 days. The DSPP protein and mRNA levels were analyzed by western blot and quantitative real-time PCR, and the mRNA levels of ALP were also analyzed by quantitative real-time PCR. The formation of mineralized nodules were examined by Alizarin red staining.

Results

Western blot analysis showed that the expression of p38β can be detected in hDPCs. Quantitative real-time PCR showed that DSPP and ALP mRNA levels were obviously decreased after stable low expression of p38β, the levels of DSPP protein also decreased. Mineralization nodules were less observed in p38β-shRNA group after the induction of odontogenic differentiation of HDPCs for 14 days.

Conclusion

P38β MAPK is involved in OM-induced odontoblastic differentiation of HDPCs.

Key words: Mitogen-activated protein kinases, Dental pulp cell, Odontoblast differentiation

Pengcheng Liu, Wei Qin, Shuheng Huang, Lingling Chen, Quan Xing, Zhengmei Lin. The role of p38β in odontoblastic differentiation of human dental pulp cells[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(01): 1-6.

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Figures/Tables 7

References 16

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基因	引物序列	大小（bp）
DSPP	F：5 ′ -TGGAGCCACAAACAGAAGCAACAC-3 ′	105
?	R：5 ′ -TCTTGGACAACAGCGACATCCTCA-3 ′	?
ALP	F：5 ′ -CCACGTCTTCACATTTGGTG-3 ′	196
?	R：5 ′ -AGACTGCGCCTGGTAGTTGT-3 ′	?
p38β	F：5 ′ -AGAAGGTGGCGGTGAAGAAG-3 ′	121
?	R：5 ′ -TCCAGAAGCCCGATGACG-3 ′	?
GAPDH	F：5 ′ -TCTCCTCTGACTTCAACAGCGACA-3 ′	126
?	R：5 ′ -CCCTGTTGCTGTAGCCAAATTCGT-3 ′	?

基因	引物序列	大小（bp）
DSPP	F：5 ′ -TGGAGCCACAAACAGAAGCAACAC-3 ′	105
?	R：5 ′ -TCTTGGACAACAGCGACATCCTCA-3 ′	?
ALP	F：5 ′ -CCACGTCTTCACATTTGGTG-3 ′	196
?	R：5 ′ -AGACTGCGCCTGGTAGTTGT-3 ′	?
p38β	F：5 ′ -AGAAGGTGGCGGTGAAGAAG-3 ′	121
?	R：5 ′ -TCCAGAAGCCCGATGACG-3 ′	?
GAPDH	F：5 ′ -TCTCCTCTGACTTCAACAGCGACA-3 ′	126
?	R：5 ′ -CCCTGTTGCTGTAGCCAAATTCGT-3 ′	?

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