The correlation between the sortaseA genotype of Streptococcus mutans and high-severity childhood caries

doi:10.3877/cma.j.issn.1674-1366.2016.04.002

Abstract

Abstract:

Objectives

To make a primary research on the relationship between genotypes of sortase A gene (srtA) of Streptococcus mutans (S.mutans) and high-severity childhood caries on the base of existenceof no other factors related to childhood caries.

Methods

The ABI Variant Reporter software was used to analyze the genotypes of the srtA gene of 121 S.mutans strains isolated from the high-severity caries children and 121 S.mutans strains isolated from the caries-free children. Bivariate analysis was used to analyze the srtA genotype distribution and the frequencies of social and behavioural variants between groups. Multiple logistic regression models were used to assess the relationship between the srtA genotype and childhood caries after controlling confounding factors.

Results

A total of 15 genotypes were found: 9 in caries free group and 11 in high-severity caries group. The frequency of 3A genotype was significantly higher in caries free group than high-severity caries group (P= 0.033) . The frequency of 3E genotype was significantly higher in high-severity caries group than caries free group (P= 0.037) . The best-fitting model showed that older age (months) , low weight at birth, high frequencies of solid sugar consumption, prolonged breastfeeding, a high proportion of visible plaque, and 3A genotype of the srtA gene of S.mutans were associated with high-severity caries in children.

Conclusions

The present study suggested that the 3A genotype of the srtA gene of S.mutans may correlate with caries susceptibility in children. In addition, age, weight at birth, duration of breastfeeding, solid sugar consumption, and poor oral hygiene contributed to primary childhood caries.

Key words: Caries, Streptococcus mutans, SrtA, Genotype

Lixia Yu, Peilin Zhuang, Huancai Lin. The correlation between the sortaseA genotype of Streptococcus mutans and high-severity childhood caries[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2016, 10(04): 238-243.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2016.04.002

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References 24

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引物	序列	产物(bp)
Primer 1	F:5′?GACGTTTGGCAACTGGTGTG?3′	557
?	R:5′?CCAAGCAATTAGGGCATTTC?3′	?
Primer 2	F:5′?CAATGAAAAAAGAACGTCAATCTA?3′	448
?	R:5′?TGTGAAGATCCGGTCATACCA?3′	?
Primer 3	F:5′?CGGAATTGCCATTCCAGACT?3′	721
?	R:5′?TCCGAAACTATCAAAGCAACAT?3′	?

引物	序列	产物(bp)
Primer 1	F:5′?GACGTTTGGCAACTGGTGTG?3′	557
?	R:5′?CCAAGCAATTAGGGCATTTC?3′	?
Primer 2	F:5′?CAATGAAAAAAGAACGTCAATCTA?3′	448
?	R:5′?TGTGAAGATCCGGTCATACCA?3′	?
Primer 3	F:5′?CGGAATTGCCATTCCAGACT?3′	721
?	R:5′?TCCGAAACTATCAAAGCAACAT?3′	?

变量		分类/记分
分类变量		?	?	?
?	性别	1:男	2:女	?
?	孕周	1:≥37周	2:<37周	?
?	分娩方式	1:顺产	2:剖腹产	?
?	出生体重	1:≥2500 g	2:<2500 g	?
?	奶瓶喂养	1:有	2:无	?
?	母乳喂养持续时间	1:没吃过母乳	2:<1年	3:≥1年
?	固体糖摄入频率	1:<1次/天	2:≥1次/天	?
?	刷牙频率	1:≥1次/天	2:<1次/天	?
?	使用牙膏	1:经常	2:有时	3:从不
?	釉质发育不全	1:无	2:有	?
?	母亲受教育程度^a	1:>12年	2:≤12年	?
?	父亲受教育程度	1:>12年	2:≤12年	?
?	母亲职业^b	1:第一类	2:第二类	3:第三类
?	父亲职业^b	1:第一类	2:第二类	3:第三类
连续变量		?	?	?
?	母亲生育孩子的年龄	?	?	?
?	月龄	36 ~ 47个月	?	?
?	可视菌斑指数(VPI)	0% ~ 100%	?	?
?	dmft	0 ~ 20	?	?
?	dmfs	0 ~ 88	?	?

变量		分类/记分
分类变量		?	?	?
?	性别	1:男	2:女	?
?	孕周	1:≥37周	2:<37周	?
?	分娩方式	1:顺产	2:剖腹产	?
?	出生体重	1:≥2500 g	2:<2500 g	?
?	奶瓶喂养	1:有	2:无	?
?	母乳喂养持续时间	1:没吃过母乳	2:<1年	3:≥1年
?	固体糖摄入频率	1:<1次/天	2:≥1次/天	?
?	刷牙频率	1:≥1次/天	2:<1次/天	?
?	使用牙膏	1:经常	2:有时	3:从不
?	釉质发育不全	1:无	2:有	?
?	母亲受教育程度^a	1:>12年	2:≤12年	?
?	父亲受教育程度	1:>12年	2:≤12年	?
?	母亲职业^b	1:第一类	2:第二类	3:第三类
?	父亲职业^b	1:第一类	2:第二类	3:第三类
连续变量		?	?	?
?	母亲生育孩子的年龄	?	?	?
?	月龄	36 ~ 47个月	?	?
?	可视菌斑指数(VPI)	0% ~ 100%	?	?
?	dmft	0 ~ 20	?	?
?	dmfs	0 ~ 88	?	?

命名	各基因型碱基突变情况
无突变	0	?	?	?
1A	470G>A	?	?	?
1B	671A>C	?	?	?
1C	671A>T	?	?	?
2A	176A>G	671A>C	?	?
2B	183G>A	671A>C	?	?
2C	256>T	671A>C	?	?
2D	470G>A	671A>C	?	?
3A	168T>G	176A>G	671A>C	?
3B	176A>G	183G>T	671A>C	?
3C	176A>G	298G>A	671A>C	?
3D	176A>G	382G>A	671A>C	?
3E	176A>G	470G>A	671A>C	?
3F	176A>G	671A>C	706G>A	?
3G	183G>A	548T>C	671A>C	?
4A	168T>G	176A>G	584C>T	671A>C

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