The experimental study on the effect of repairing mandibular defects with CHA/rhBMP-2 by individualized titanium bracket

doi:10.3877/cma.j.issn.1674-1366.2012.04.006

Abstract

Abstract:

Objective

To study the effect of individualized titanium bracket combined with coralline hydroxyapatite （CHA） and recombinant human bone morphogenetic protein-2 （rhBMP-2） on bone defect repairing.

Methods

Radial critical size bone defect models were made on 9 rabbits，Individualized titanium bracket were obtained from CAD/CAM and RP. X-ray， histological examination，biomechanics analysis and immunohistochemical staining were made 4， 12， and 24 weeks post operation.

Results

The integrity of the mandible was ideally recovered. New bone formation was seen in both the X-ray observation and the histological examination （P＜0.05）. The best osseo-integrity can be observed at 24th week.

Conclusion

It is possible to obtain individualized functional reconstruction of mandibular segmental defect using individualized three-dimensional titanium bracket combined with CHA and rhBMP-2.

Key words: Mandibular defect, Coralline hydroxyapatite, Human bone morphogenetic protein-2, Individualized titanium bracket

Zhong-dong WANG, Jun-lan CHEN, Jue-yao CHEN, Wen HU, Zhen-yu GONG, Ya-xiong LIU, Shao-Zhen LING, Ji-nan WU. The experimental study on the effect of repairing mandibular defects with CHA/rhBMP-2 by individualized titanium bracket[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 6(04): 336-341.

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Figures/Tables 8

References 11

1	Liao Y， Song J， Li E， et al. Rapid prototyping of threedimensional microfluidic mixers in glass by femtosecond laser direct writing. Lab Chip， 2012，12（4）：746-749.
2	Takahata M， Awad HA， O'Keefe RJ， et al. Endogenous tissue engineering： PTH therapy for skeletal repair. Cell Tissue Res，2012，347（3）：545-552.
3	Lu JX， Flautre B， Anselme K， et al. Role of interconnections in porous bioceramics on bone recolonization in vitro and in vivo. J Mater Sci Mater Med， 1999，10（2）：111-120.
4	李俊，孟志斌，周键强，等. 珊瑚羟基磷灰石人工骨移植修复良性骨肿瘤骨缺损32 例. 中国组织工程研究与临床康复，2008，12（36）：7085-7088.
5	Owens K， Glassman SD， Howard JM， et al. Perioperative complications with rhBMP-2 in transforaminal lumbar interbody fusion. Eur Spine J， 2011，20（4）：612-617.
6	Smoljanovic T， Bojanic I. An evolving perception of the risk of rhBMP-2 use for anterior spinal interbody fusions. Spine J，2011，11（6）：520-521.
7	Pereira NT，Issa JP，Nascimento Cd，et al.Effect of alveolex on the bone defects repair stimulated by rhBMP-2： Histomorphometric study. Microsc Res Tech， 2012，75（1）：36-41.
8	Liu Y， Lu Y， Tian X， et al. Segmental bone regeneration using an rhBMP-2-loaded gelatin/nanohydroxyapatite/fibrin scaffold in a rabbit model. Biomaterials， 2009，30（31）：6276-6285.
9	Xu H， Han D， Dong JS， et al. Rapid prototyped PGA/PLA scaffolds in the reconstruction of mandibular condyle bone defects. Int J Med Robot， 2010，6（1）：66-72.
10	Sabol JV， Grant GT， Liacouras P， et al. Digital image capture and rapid prototyping of the maxillofacial defect. J Prosthodont，2011，20（4）：310-314.
11	Hoque ME， Chuan YL， Pashby I. Extrusion based rapid prototyping technique： an advanced platform for tissue engineering scaffold fabrication. Biopolymers， 2012，97（2）：83-93.

时间（周）	骨密度（g/cm²）	生物力学（N）
4	0.373±0.001	191.67±3.786
12	0.472±0.004^a	241.67±9.609^a
24	0.513±0.011^b	322.67±13.868^b

时间（周）	骨密度（g/cm²）	生物力学（N）
4	0.373±0.001	191.67±3.786
12	0.472±0.004^a	241.67±9.609^a
24	0.513±0.011^b	322.67±13.868^b

时间（周）	Ⅰ型胶原	骨钙素
4	0.257±0.076	0.353±0.100
12	0.421±0.083^a	0.473±0.005^a
24	0.505±0.145^b	0.537±0.102^b

时间（周）	Ⅰ型胶原	骨钙素
4	0.257±0.076	0.353±0.100
12	0.421±0.083^a	0.473±0.005^a
24	0.505±0.145^b	0.537±0.102^b

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