The clinical significance of the expression of CD133 in oral lichen planus and oral squamous cell carcinoma

doi:10.3877/cma.j.issn.1674-1366.2016.05.004

Abstract

Abstract:

Objective

To investigate the expression of CD133 in oral normal mucosa, oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC) , to analyze the relationship between its expression and the clinical factors, and to evaluate the significnce of CD133 as an index to evaluate the canceration potency of OLP.

Methods

Immunohistochemical analysis and Mann-Whiney test were applied to investigate the expression of CD133 in paraffin section of human oral normal mucosa, OLP and OSCC tissues. Then the relationships between its expression and clinical factors were analyzed. Immunohistochemical analysis, Western blot analysis and t test were also applied to investigate the expression of CD133 in human dysplastic oral keratinocyte (DOK) cell line and human oral squamous cell carcinoma (CAL-27) cell line.

Results

A gradual increase in the expression of CD133 was observed in three groups, the positive rates of CD133 were 0 (0/10) , 31.67% (19/60) , 63.33% (38/60) respectively. Significant differences were observed in expression of CD133 in oral normal mucosa and OLP (Z=-2.046, P= 0.041) , OLP and OSCC (Z=-3.777, P<0.001) . An increase in the expression of CD133 were also observed from DOK cell line to CAL-27 cell line (t=-5.029, P= 0.001) .

Conclusion

CD133 can be used as clinical index to evaluate the canceration potency of oral lichen planus.

Key words: Mouth mucosa, Lichen planus, Carcinoma, squamous cell, CD133

Yan Sun, Jiajia Qi, Changqing Yuan, Quanchen Xu, Han Zhao, Yuanpan Cao, Qiuyan Liu. The clinical significance of the expression of CD133 in oral lichen planus and oral squamous cell carcinoma[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2016, 10(05): 322-326.

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https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2016/V10/I05/322

Figures/Tables 5

References 18

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组别	例数	CD133				Z值	P值
组别	例数	-	+	++	+++	Z值	P值
正常口腔黏膜组	10	10	0	0	0	-	-
OLP组	60	41	12	7	0	-2.046	0.041
OSCC组	60	22	17	15	6	-3.777	0.000

组别	例数	CD133				Z值	P值
组别	例数	-	+	++	+++	Z值	P值
正常口腔黏膜组	10	10	0	0	0	-	-
OLP组	60	41	12	7	0	-2.046	0.041
OSCC组	60	22	17	15	6	-3.777	0.000

临床资料		例数	CD133		χ²值	P值
临床资料		例数	阴性	阳性	χ²值	P值
年龄（岁）		?	?	?	0.095	0.758
?	≥60	73	39	34	?	?
?	<60	57	32	25	?	?
性别		?	?	?	0.022	0.882
?	男	89	49	40	?	?
?	女	41	22	19	?	?
吸烟史		?	?	?	0.738	0.390
?	无	56	33	23	?	?
?	有	74	38	36	?	?
饮酒史		?	?	?	0.410	0.522
?	无	49	25	24	?	?
?	有	81	46	35	?	?
病变部位		?	?	?	7.961	0.158
?	舌	37	21	16	?	?
?	颊	28	13	15	?	?
?	腭	5	2	3	?	?
?	唇	13	6	7	?	?
?	牙龈	21	9	12	?	?
?	口底	26	20	6	?	?
临床分期		?	?	?	5.967	0.015
?	Ⅰ~Ⅱ期	37	18	19	?	?
?	Ⅲ~Ⅳ 期	23	4	19	?	?
淋巴结转移		?	?	?	7.232	0.007
?	无	42	20	22	?	?
?	有	18	2	16	?	?

临床资料		例数	CD133		χ²值	P值
临床资料		例数	阴性	阳性	χ²值	P值
年龄（岁）		?	?	?	0.095	0.758
?	≥60	73	39	34	?	?
?	<60	57	32	25	?	?
性别		?	?	?	0.022	0.882
?	男	89	49	40	?	?
?	女	41	22	19	?	?
吸烟史		?	?	?	0.738	0.390
?	无	56	33	23	?	?
?	有	74	38	36	?	?
饮酒史		?	?	?	0.410	0.522
?	无	49	25	24	?	?
?	有	81	46	35	?	?
病变部位		?	?	?	7.961	0.158
?	舌	37	21	16	?	?
?	颊	28	13	15	?	?
?	腭	5	2	3	?	?
?	唇	13	6	7	?	?
?	牙龈	21	9	12	?	?
?	口底	26	20	6	?	?
临床分期		?	?	?	5.967	0.015
?	Ⅰ~Ⅱ期	37	18	19	?	?
?	Ⅲ~Ⅳ 期	23	4	19	?	?
淋巴结转移		?	?	?	7.232	0.007
?	无	42	20	22	?	?
?	有	18	2	16	?	?

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