The inhibitory effect of proanthocyanidins on the activities of gelatinase/collagenase in dentin matrix

doi:10.3877/cma.j.issn.1674-1366.2013.04.005

Abstract

Abstract:

Objective

To evaluate the potential inhibitory effect of proanthocyanidins （PA）from grape seeds extract on the activities of endogenous gelatinase and collagenase in demineralized dentin matrix.

Methods

PA-based preconditioners were prepared by adding powdered PA-rich grape seeds extract to aqueous ethanol at various concentration. Extracted intact human third molars were collected， powdered in liquid nitrogen and pretreated by PA preconditioners at various concentration（15% PA or 10% PA） and for different pretreatment durations （60 s or 120 s）. All the specimens were thoroughly rinsed by repeated centrifugation， kept drying and lyophilized. EnzChek gelatinase/collagenase assay was carried out to investigate the activities of gelatinase and collagenase in demineralized dentin matrix.

Results

Fluorescence attributed to the activities of gelatinase and collagenase gradually increased along the reaction. When dentin powder was pretreated by 10% PA， 15% PA （for either pretreatment duration）， both the activities of gelatinase and collagenase were greatly inhibited in comparison to the non-treated control （P＜0.05）.

Conclusion

PA pretreatment was remarkably effective in silencing the activities of endogenous gelatinase/collagenase in demineralized dentin.

Key words: Proanthocyanidins, Dentin, Gelatinolysis/collagenolysis degradation

Rui-rui LIU, Ke-qiang MA, Qi DOU, Cheng-fang TANG, Ming FANG, Ji-hua CHEN. The inhibitory effect of proanthocyanidins on the activities of gelatinase/collagenase in dentin matrix[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2013, 07(04): 283-287.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2013.04.005

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2013/V07/I04/283

Figures/Tables 3

References 21

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