Effect of tannic acid with different concentrations on bond strength under different etching modes

doi:10.3877/cma.j.issn.1674-1366.2020.04.004

Abstract

Abstract:

Objective

To evaluate the effect of tannic acid with various concentrations on the bond strength between dentin and universal adhesive under the total and self-etching modes.

Methods

Sixty human molars without caries were randomly divided into two groups (group A: total-etching mode; group B: self-etching mode, n = 30) with five subgroups (n = 6) from the box blindly. After exposing its dentine, tannic acid with different concentrations of 0% (control group) , 25%, 50%, 75% and 100% was applied on dentin surface for 3 minutes with rinsing and air-blowing before Single Bond Universal (SBU) was used. Micro-tensile Bond Strength test (μTBSt) was carried out after 37 ℃ water-storage for 24 h. The data were analyzed by Two-Way ANOVA and Games-Howell test (α = 0.05) . Failure mode analysis and fracture surface observation on dentin side were done by stereomicroscope and electron scanning microscope (SEM) , respectively.

Results

In the total-etching mode, the bond strength of 75% and 100% concentration groups was significantly higher than that of the other groups (P<0.05) . In the self-etching mode, significantly higher bond strength than the rest was achieved by 25% concentration (P<0.05) . Two-Way ANOVA revealed a significant effect of tannic acid concentration (F = 23.134, P<0.001) and etching mode (F = 4.465, P = 0.036) on bond strength, while the interaction between two variables was also significant (F = 28.231, P<0.001) . The results of failure mode analysis and SEM observation indicated that the interfacial morphology on dentin created by both etching modes and different concentrations of tannic acid was significantly different.

Conclusion

The bond strength between dentin and SBU could be significantly improved by tannic acid with different concentrations in both total and self-etching modes.

Key words: Tannins, Dentin, Acid etching, Tensile Strength, Universal adhesive system, Single Bond Universal, Micro-tensile bond strength test

Yuqing Bian, Yidan Ma, Dongyue Dai, Ping Su, Mengqi Jia, Desong Zhan, Jiale Fu. Effect of tannic acid with different concentrations on bond strength under different etching modes[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2020, 14(04): 221-227.

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References 31

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单宁酸浓度	全酸蚀组			自酸蚀组
单宁酸浓度	内部断裂	粘接面断裂	混合断裂	内部断裂	粘接面断裂	混合断裂
0%	24（33.3）	36（50.0）	12（16.7）	56（77.8）	14（19.4）	2（2.8）
25%	48（66.7）	22（30.5）	2（2.8）	47（65.3）	18（25.0）	7（9.7）
50%	32（44.4）	39（54.2）	1（1.4）	54（75.0）	9（12.5）	9（12.5）
75%	35（48.6）	31（43.1）	6（8.3）	46（63.9）	23（31.9）	3（4.2）
100%	42（58.3）	22（30.6）	8（11.1）	41（56.9）	27（37.5）	4（5.6）
总计	181（50.3）	150（41.7）	29（8.0）	244（67.8）	91（25.3）	25（6.9）

单宁酸浓度	全酸蚀组			自酸蚀组
单宁酸浓度	内部断裂	粘接面断裂	混合断裂	内部断裂	粘接面断裂	混合断裂
0%	24（33.3）	36（50.0）	12（16.7）	56（77.8）	14（19.4）	2（2.8）
25%	48（66.7）	22（30.5）	2（2.8）	47（65.3）	18（25.0）	7（9.7）
50%	32（44.4）	39（54.2）	1（1.4）	54（75.0）	9（12.5）	9（12.5）
75%	35（48.6）	31（43.1）	6（8.3）	46（63.9）	23（31.9）	3（4.2）
100%	42（58.3）	22（30.6）	8（11.1）	41（56.9）	27（37.5）	4（5.6）
总计	181（50.3）	150（41.7）	29（8.0）	244（67.8）	91（25.3）	25（6.9）

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