Preliminary study on resin-dentin bond durability by alginate dialdehyde preconditioning

doi:10.3877/cma.j.issn.1674-1366.2018.06.001

Abstract

Abstract:

Objective

To investigate the effect of alginate dialdehyde (ADA) on demineralized dentin substrate, in its cross-linking degree, micromorphology, inhibition ability and resin-dentin bonding strength.

Methods

ADA was prepared, and analyzed by Fourier Transform infrared spectroscopy (FTIR) . Demineralized dentin powder were treated by 0.5%, 1%, 2%, 3%, 4% ADA for 1, 2, 30 min, respectively, and examined by Nihydri colorimetry for the cross-linking degrees, 5% Glutaraldehyde was used as positive control (1.0 ± 0.1) mm dentin discs were etched, rinsed and pretreated with ADA successively, and kept dry or moist for field emission scanning electron microscope (FESEM) . The inhibition of ADA on MMP-9 was analyzed by Sensolyte Generic MMP assay kit. Resin-dentin bonding specimens were prepared for micro-tensile bond strength evaluation (MTBS) , before and after thermolcycling. Data were analyzed by Multi-way ANOVA, Kruskal-Wallis test or One-way ANOVA by SPSS 22.0 software package.

Results

FTIR analysis confirmed the characteristic peak of aldehyde group in ADA. The cross-linking degree of ADA-treated DDP was exhibited concentration- and time-dependent and from which significant differences were found among different ADA concentration (F = 1329.423, P<0.001) and different time (F = 1142.93, P<0.001) . The FESEM result confirmed that the demineralized dentin collagen was in a homogenous and regular arrangement after ADA or GD treatment, except 0.5% ADA dry group. Over 93.5% of the MMP-9 was inhibited by 0.5% ~ 4% ADA (F = 13.786, P<0.001) . The 2% ADA group exhibited highest MTBS before [ (28.2 ± 4.2) MPa] (F = 5.544, P<0.001) and after [ (18.3 ± 3.7) MPa] (F = 5.181, P<0.001) thermolcycling.

Conclusions

2% ADA could increase cross-linking degree of demineralized dentin substrate, keep collagen fiber from collapse, and inhibit the activity of MMP-9, thus increase the durability of the dentin adhensive.

Key words: Alginates, Cross-linking reagents, natural, Resin-bonded, dentin, Alginate dialdehyde

Dandan Xie, Sui Mai, Qian Wu, Manduo Zhao, Yi Tan, Lisha Gu. Preliminary study on resin-dentin bond durability by alginate dialdehyde preconditioning[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2018, 12(06): 329-334.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2018.06.001

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2018/V12/I06/329

Figures/Tables 5

References 23

[1]	Sano H, Shono T, Takatsu T, et al. Microporous dentin zone beneath resin-impregnated layer [J]. Oper Dent, 1994, 19(2):59-64.
[2]	Ritter AV, Bertoli C, Swift EJ Jr. Dentin bond strengths as a function of solvent and glutaraldehyde content [J]. Am J Dent, 2001, 14(4):221-226.
[3]	Bouhadir KH, Lee KY, Alsberg E, et al. Degradation of partially oxidized alginate and its potential application for tissue engineering [J]. Biotechnol Prog, 2001, 17(5):945-950.
[4]	Xu Y, Huang C, Li L, et al. In vitro enzymatic degradation of a biological tissue fixed by alginate dialdehyde [J]. Carbohydr Polym, 2013, 95(1):148-154.
[5]	Xu Y, Li L, Yu X, et al. Feasibility study of a novel crosslinking reagent（alginate dialdehyde）for biological tissue fixation [J]. Carbohydr Polym, 2012, 87(2):1589-1595.
[6]	Hu Y, Liu L, Gu Z, et al. Modification of collagen with a natural derived cross-linker, alginate dialdehyde [J]. Carbohydr Polym, 2014(102):324-332.
[7]	Mazzoni A, Mannell F, Tay FR, et al. Zymographic Analysis and Characterization of MMP-2 and -9 forms in human sound dentin [J]. J Dent Res, 2007, 86(5):436-440.
[8]	Ito S, Hashimoto M, Wadgonkar B, et al. Effects of resin hydrophilicity on water sorption and changes in modulus of elasticity [J]. Biomaterials, 2005, 26(33):6449-6459.
[9]	Sadek FT, Pashley DH, Nishitani Y, et al. Application of hydrophobic resin adhesives to acid-etched dentin with an alternative wet bonding technique [J]. J Biomed Mater Res A, 2008, 84(1):19-29.
[10]	Kim YK, Gu LS, Bryan TE, et al. Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein analogues in the presence of calcium, phosphate and hydroxyl ions [J]. Biomaterials, 2010, 31(25):6618-6627.
[11]	Mai S, Kim YK, Kin J, et al. In vitro remineralization of severely compromised bonded dentin [J]. J Dent Res, 2010, 89(4):405-410.
[12]	Gendron R, Grenier D, Sorsa T, et al. Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine [J]. Clin Diagn Lab Immunol, 1999, 6(3):437-439.
[13]	Scaffa PM, Vidal CM, Barros N, et al. Chlorhexidine Inhibits the Activity of Dental Cysteine Cathepsins [J]. J Dent Res, 2012, 91(4):420-425.
[14]	Blackburn RS, Harvey A, Kettle LL, et al. Sorption of chlorhexidine on cellulose：mechanism of binding and molecular recognition [J]. J Phys Chem B, 2007, 111(30):8775-8784.
[15]	Castellan CS, Pereira PN, Grande RH, et al. Mechanical characterization of proanthocyanidin-dentin matrix interaction [J]. Dent Mater, 2010, 26(10):968-973.
[16]	Deng M, Dong X, Zhou X, et al. Characterization of dentin matrix biomodified by Galla chinensis extract [J]. J Endod, 2013, 39(4):542-547.
[17]	Kim GE, Leme-Kraus AA, Phansalkar R, et al. Effect of bioactive primers on bacterial-induced secondary caries at the tooth-resin [J]. Oper Dent, 2017, 42(2):196-202.
[18]	Silva AP, Gonçalves RS, Borges AF, et al. Effectiveness of plant-derived proanthocyanidins on demineralization on enamel and dentin under artificial cariogenic challenge [J]. J Appl Oral Sci, 2015, 23(3):302-309.
[19]	Epasinghe DJ, Yiu CKY, Burrow MF, et al. Effect of proanthocyanidin incorporation into dental adhesive on durability of resin-dentin bond [J]. Int J Adhes Adhes, 2015(63):145-151.
[20]	Le-Tien C, Millette M, Lacroix M, et al. Modified alginate matrices for the immobilization of bioactive agents [J]. Biotechnol Appl Biochem, 2004, 39(Pt 2):189-198.
[21]	Mazzoni A, Pashley DH, Nishitani Y, et al. Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives [J]. Biomaterials, 2006, 27(25):4470-4476.
[22]	De Munck J, Mine A, Van den Steen PE, et al. Enzymatic degradation of adhesive-dentin interfaces produced by mild self-etch adhesives [J]. Eur J Oral Sci, 2010, 118(5):494-501.
[23]	Liu Y, Tjäderhane L, Breschi L, et al. Limitations in bonding to dentin and experimental strategies to prevent bond degradation [J]. J Dent Res, 2011, 90(8):953-968.

组别	1 min	2 min	30 min
5% GD组	55.4 ± 2.8^Aa	84.5 ± 0.9^Ba	97.0 ± 1.0^Ca
0.5% ADA组	2.5 ± 0.9^Ab	15.6 ± 2.9^Bb	21.6 ± 3.7^Cb
1% ADA组	8.2 ± 2.9^Ac	21.5 ± 2.2^Bc	30.6 ± 4.6^Cc
2% ADA组	19.4 ± 3.0^Ad	21.6 ± 2.9^Ac	36.3 ± 3.7^Bd
3% ADA组	21.5 ± 2.6^Ad	24.0 ± 3.9^Ac	38.7 ± 5.4^Bd
4% ADA组	22.1 ± 2.9^Ad	24.0 ± 3.5^Ac	46.4 ± 5.4^Be

组别	1 min	2 min	30 min
5% GD组	55.4 ± 2.8^Aa	84.5 ± 0.9^Ba	97.0 ± 1.0^Ca
0.5% ADA组	2.5 ± 0.9^Ab	15.6 ± 2.9^Bb	21.6 ± 3.7^Cb
1% ADA组	8.2 ± 2.9^Ac	21.5 ± 2.2^Bc	30.6 ± 4.6^Cc
2% ADA组	19.4 ± 3.0^Ad	21.6 ± 2.9^Ac	36.3 ± 3.7^Bd
3% ADA组	21.5 ± 2.6^Ad	24.0 ± 3.9^Ac	38.7 ± 5.4^Bd
4% ADA组	22.1 ± 2.9^Ad	24.0 ± 3.5^Ac	46.4 ± 5.4^Be

组别	例数	未经冷热循环	冷热循环后
阴性对照组	30	27.2 ± 3.3^A	14.4 ± 3.4^a
5% GD组	30	29.4 ± 3.1^B	17.9 ± 4.2^bc
0.5% ADA组	30	27.4 ± 4.0^A	16.6 ± 3.4^bc
1% ADA组	30	27.1 ± 3.7^A	18.0 ± 3.0^bc
2% ADA组	30	28.2 ± 4.2^AB	18.3 ± 3.7^b
3% ADA组	30	27.1 ± 3.5^A	16.3 ± 3.4^c
4% ADA组	30	24.2 ± 4.1^C	-

组别	例数	未经冷热循环	冷热循环后
阴性对照组	30	27.2 ± 3.3^A	14.4 ± 3.4^a
5% GD组	30	29.4 ± 3.1^B	17.9 ± 4.2^bc
0.5% ADA组	30	27.4 ± 4.0^A	16.6 ± 3.4^bc
1% ADA组	30	27.1 ± 3.7^A	18.0 ± 3.0^bc
2% ADA组	30	28.2 ± 4.2^AB	18.3 ± 3.7^b
3% ADA组	30	27.1 ± 3.5^A	16.3 ± 3.4^c
4% ADA组	30	24.2 ± 4.1^C	-

Please choose a citation manager

Content to export