Abstract:
Objective
To clone the full-length human bone morphogenetic protein-2（BMP-2） and to construct the eukaryotic expression vector of pcDNA3.1 （+）/BMP-2.
Methods
Total RNA was isolated from human condoyle osseous tissue according to Trizol method. The fulllength human BMP-2 were obtained by reverse transcription PCR， and cloned into the eucaryotic expression plasmid of pcDNA3.1 （+）.
Results
The result of PCR， digesting and sequencing demonstrated that the BMP-2 gene was cloned successfully and the eukaryotic expression vector of pcDNA3.1 （+ ）-BMP-2 was constructed correctly.
Conclusions
The BMP-2 cloned into eukaryotic expression vector pcDNA3.1 （+）/BMP-2 was constructed correctly， which provided basis for further study of BMP-2 and its expression in human mesenchymal stem cell （hMSCs）.
Key words:
Bone morphogenetic protein 2,
Gene cloning,
Eukaryotic expression vector
Jianxin JI, Wei-jiao LIAO, Li-dong LIU, Shi-cong XUE. Cloning of human BMP-2 gene and constructing of eucaryotic expression plasmid[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2008, 02(05): 452-456.