Effects of mechanical strain on bone morphogenetic protein 2 concentration of osteoblasts

doi:10.3877/cma.j.issn.1674-1366.2011.05.007

Abstract

Abstract:

Objectives

To investigate the effect of different magnitudes of mechanical strain on bone morphogenetic protein 2 （BMP-2） protein expression in osteoblasts.

Methods

Rats osteoblasts were exposed to different mechanical strains. The elongation in Group A （control group）was 0%， while Group B was 6%， Group C was 12%， and Group D was 18%， respectively. Ezymelinked immunosorbnent assay （ELISA） was used to detect the expression of BMP-2 protein. Data were analyzed by SPSS 13.0 system using LSD analysis.

Results

There were significant differences among three experimental groups and the control group （P＜0.01）， and Group C expressed the highest level of BMP-2 protein.

Conclusions

Mechanical strain can promote the expression of BMP-2 protein， which might affect bone remodeling in orthodontic treatment.

Key words: Mechanical strain, Osteoblast, Bone morphogenetic protein 2

Hong-fei LU, Zhi-hui MAI, Hong AI, Ye XU, Wei WANG, Qi CHEN. Effects of mechanical strain on bone morphogenetic protein 2 concentration of osteoblasts[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2011, 5(05): 488-493.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2011.05.007

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2011/V5/I05/488

Figures/Tables 6

References 16

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组别	A ₄₅₀
组别	孔1	孔2	孔3	孔4	孔5	孔6
A 组	0.320	0.316	0.317	0.323	0.296	0.332
B 组	0.405	0.390	0.404	0.403	0.427	0.405
C 组	0.561	0.510	0.541	0.553	0.518	0.547
D 组	0.497	0.484	0.486	0.494	0.470	0.487

组别	A ₄₅₀
组别	孔1	孔2	孔3	孔4	孔5	孔6
A 组	0.320	0.316	0.317	0.323	0.296	0.332
B 组	0.405	0.390	0.404	0.403	0.427	0.405
C 组	0.561	0.510	0.541	0.553	0.518	0.547
D 组	0.497	0.484	0.486	0.494	0.470	0.487

组别	BMP-2 浓度
A 组	246.33 ± 11.73
B 组	334.67 ± 11.33
C 组	467.33 ± 19.87
D 组	415.33 ± 9.40

组别	BMP-2 浓度
A 组	246.33 ± 11.73
B 组	334.67 ± 11.33
C 组	467.33 ± 19.87
D 组	415.33 ± 9.40

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