The role of survivin in nicotine-mediated inhibition of apoptosis induced by cisplatin in tongue squamous cell carcinoma cells

doi:10.3877/cma.j.issn.1674-1366.2007.02.005

Abstract

Abstract:

Objective

Oral cancer demonstrates a strong epidemiological association with smoking. Nicotine， a major component of cigarette smoke， can regulate cell proliferation and angiogenesis and suppress apoptosis induced by chemotherapeutic drugs. But little is known about the effect of nicotine on oral cancer cell apoptosis.

Methods

Cisplatin-induced apoptosis of human oral cancer cells （Tca8113） were rendered quiescent by serum starvation for 24 h. The effect of nicotine on apoptosis was observed by TUNEL assay， Western blotting and RNA interfering technology.

Results

Nicotine inhibited apoptosis induced by cisplatin in Tca8113 cells. Survivin took part in the inhibiting apoptosis process of nicotine in human oral cancer Tca8113 cells. Depletion of survivin by the siRNA ablated the protective effects of nicotine against cisplatin-induced apoptosis. Meanwhile， Akt pathway is necessary for the antiapoptotic effect of nicotine. LY294002 （an antagonist of Akt pathway） ablated the antiapoptotic effect of nicotine and inhibited the induction of survivin by nicotine in Tca8113 cells.

Conclusions

Exposure to nicotine might negatively impact the apoptotic potential of chemotherapeutic drugs. Survivin plays a key role in the antiapoptotic activity of nicotine. The Akt pathway may be required for nicotine function.

Key words: Nicotine, Oral cancer, Apoptosis, Survivin

Jian-hui XU, Hong-zhang HUANG, Chao-bin PAN, Bin ZHANG, Jian-guang WANG, Lei-tao ZHANG. The role of survivin in nicotine-mediated inhibition of apoptosis induced by cisplatin in tongue squamous cell carcinoma cells[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2007, 01(02): 78-82.

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