The effect of brain derived neurotrophin factor on the proliferation and anoikis resistance of salivary gland adenoid cystic carcinoma cell line in vitro

doi:10.3877/cma.j.issn.1674-1366.2007.02.006

Abstract

Abstract:

Objective

To observe the effect of BDNF on the proliferation and anoikis resistance of salivary gland adenoid cystic carcinoma （SACC） cell line in vitro.

Methods

MTT， suspension culture system， FCM， clone formation test were used on ACC-2 and ACC-M （high lung metastasis strain） to detect the ability of anoikis resistance of SACC cell line and the effect of BDNF on the proliferation and anoikis resistance of SACC cell line.

Results

Both ACC-2 and ACC-M had the ability of anoikis resistance and showed correlation with the ability of metastasis （P＜0.01）. BDNF at different concentration （25， 50， 100 ng/ml） did not affect the proliferation of ACC-2 and ACC-M （P ＞0.05）.BDNF at concentration of 50 ng/ml could improve the clone formation of ACC-2 and ACC-M （P＜0.01）.

Conclusion

Anoikis resistance had correlation with the metastasis ability of SACC. BDNF at proper concentration could improve the anoikis resistance of SACC cell line.

Key words: Salivary gland adenoid cystic carcinoma, brain derived neurotrophin factor, Anoikis, Metastasis

Li-xin SU, Chen-ping ZHANG. The effect of brain derived neurotrophin factor on the proliferation and anoikis resistance of salivary gland adenoid cystic carcinoma cell line in vitro[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2007, 01(02): 83-86.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2007.02.006

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Figures/Tables 1

References 11

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