Abstract:
Objective
To investigate the effect of static compression on the proliferation activity，apoptosis rate， and matrix proteoglycans synthesis of cultured mandibular condylar cartilage（MCC）cells， discuss the relation between abnormal compression and degradation of cartilage.
Methods
MCC cells were harvested from 2-week-old SD rats by enzymatic method， and cultured in DMEM medium containing 10% fetal serum. MCC cells of the second generation were placed into containers. These containers were sealed and perfused with 5% CO2 to offer -10、10、20 kPa static compression respectively， No mechanical loads were applied to the control group. 3 h and 24 h after loading，proliferation activity and apoptosis rate were determined by MTT method and flowcytometry respectively，the content of matrix proteoglycans were determined by sulfuric acid-carbazole colorimetry methods.
Results
When the peak stress reached 20 kPa and maintained for more than 24 h， the proliferation activity and the synthesis matrix proteoglycans were significantly decreased， the apoptosis rate was significantly increased.
Conclusion
Abnormal compression induces apoptosis of chondrocytes and reduction of the content of proteoglycans may trigger or deteriorate the degradation of cartilage.
Key words:
Chondrocytes,
Mandibular condylar,
Compression,
Apoptosis
Zhi-guang HANG, Ming-ting LIAO, Kai SU, Shi-jun KUANG, Juan ZHANG. Compression induce apoptosis of condylar cartilage chondrocytes in vitro[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2007, 01(02): 87-89.