Effects of two sterilization methods on the thermo-gelation behavior and in vitro protein release characteristics of chitosan hydrogel

doi:10.3877/cma.j.issn.1674-1366.2015.01.007

Abstract

Abstract:

Objective

To compare the effects of two different sterilization methods on the thermo-gelation behavior and protein release characteristics of chitosan hydrogel in vitro.

Methods

Chitosan thermo-sensitive hydrogel was routinely fabricated and sterilized by two methods: (1) conventional group: steam sterilization on chitosan solution; (2) modified group: steam sterilization on chitosan powder. Gelation time was recorded and the viscosity was measured using a viscometer. The topography and the cross section of the chitosan hydrogel were observed by a scanning electron microscopy (SEM) . Fourier transform infrared (FTIR) spectra were obtained with a Fourier Transform IR Spectrophotometer. In vitro protein release characteristics were observed using bovine serum albumin (BSA) as a model protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to determine the structural integrity of model protein.

Results

At 37℃, the gelation time of modified group was significantly longer than that of conventional group (t=61.677, P= 0.000) . The viscosity of modified group was 15000 mPa.s at 37℃, while the viscosity of conventional group was 1560 mPa.s at 37℃ and increased to 11 500 mPa.s at 40℃. SEM observation showed three-dimensional mesh porous structures in both groups. Mean pore size of conventional group was larger than those of modified group. Both groups presented great controlled-release of the model protein, modified group was better than conventional group (t= 61.415, P= 0.000) . There is no significant difference on the FTIR spectra of the two sterilized groups. The result of SDS-PAGE indicated that the protein released from both groups remained structural integrity.

Conclusions

Autoclaving chitosan powder had shorter gelation time, better thermo-gelation behavior and could prolong the release of model protein in vitro than autoclaving chitosan/β-GP solution.

Key words: Chitosan, Thermo-sensitive hydrogel, Sterilization methods, Protein release

Sisi Lin, Xinchun Zhang, Anxun Wang, Can Zhang, Yan Wang. Effects of two sterilization methods on the thermo-gelation behavior and in vitro protein release characteristics of chitosan hydrogel[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(01): 44-49.

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Figures/Tables 7

References 16

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组别	成胶外观	pH值	成胶时间（min）
传统组	黄色凝胶	6.97 ± 0.02	24.13 ± 1.13^a
改良组	淡黄色凝胶	7.01 ± 0.01	8.00 ± 0.76^a

组别	成胶外观	pH值	成胶时间（min）
传统组	黄色凝胶	6.97 ± 0.02	24.13 ± 1.13^a
改良组	淡黄色凝胶	7.01 ± 0.01	8.00 ± 0.76^a

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