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Chinese Journal of Stomatological Research(Electronic Edition) ›› 2015, Vol. 09 ›› Issue (01): 44-49. doi: 10.3877/cma.j.issn.1674-1366.2015.01.007

Special Issue:

• Basic Science Research • Previous Articles     Next Articles

Effects of two sterilization methods on the thermo-gelation behavior and in vitro protein release characteristics of chitosan hydrogel

Sisi Lin1, Xinchun Zhang1, Anxun Wang2, Can Zhang1, Yan Wang1,()   

  1. 1. Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
    2. Department of Stomatology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
  • Received:2014-12-31 Online:2015-02-01 Published:2015-02-01
  • Contact: Yan Wang
  • About author:
    Corresponding author: Wang Yan, Email: , Tel: 020-83802805

Abstract:

Objective

To compare the effects of two different sterilization methods on the thermo-gelation behavior and protein release characteristics of chitosan hydrogel in vitro.

Methods

Chitosan thermo-sensitive hydrogel was routinely fabricated and sterilized by two methods: (1) conventional group: steam sterilization on chitosan solution; (2) modified group: steam sterilization on chitosan powder. Gelation time was recorded and the viscosity was measured using a viscometer. The topography and the cross section of the chitosan hydrogel were observed by a scanning electron microscopy (SEM) . Fourier transform infrared (FTIR) spectra were obtained with a Fourier Transform IR Spectrophotometer. In vitro protein release characteristics were observed using bovine serum albumin (BSA) as a model protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to determine the structural integrity of model protein.

Results

At 37℃, the gelation time of modified group was significantly longer than that of conventional group (t=61.677, P= 0.000) . The viscosity of modified group was 15000 mPa.s at 37℃, while the viscosity of conventional group was 1560 mPa.s at 37℃ and increased to 11 500 mPa.s at 40℃. SEM observation showed three-dimensional mesh porous structures in both groups. Mean pore size of conventional group was larger than those of modified group. Both groups presented great controlled-release of the model protein, modified group was better than conventional group (t= 61.415, P= 0.000) . There is no significant difference on the FTIR spectra of the two sterilized groups. The result of SDS-PAGE indicated that the protein released from both groups remained structural integrity.

Conclusions

Autoclaving chitosan powder had shorter gelation time, better thermo-gelation behavior and could prolong the release of model protein in vitro than autoclaving chitosan/β-GP solution.

Key words: Chitosan, Thermo-sensitive hydrogel, Sterilization methods, Protein release

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