Abstract:

Objective

Observation of using RNA interference technology by periodontal ligament（PDL） cells calcium binding protein S100A₄ expression， to obtain stable cell monoclonal， for follow-up tests provide cellular level platform.

Methods

Used the technology of RNA interference （RNAi） to cut down S100A₄ expression， targeting the coding sequence of human S100A₄ gene and a negative control were designed. Then they were ligated into the vector of pGenesil-1 respectively，and were transfected into PDL cells. By the means of RT-PCR and Western blot，we could examine their suppression.

Result

The results showed that the interference effects of the RNAi expression vectors were 67.83%.

Conclusion

RNA interference can be effectively suppressed in periodontal ligament cells and S100A₄ gene expression. This study provides a new means for investigating the effects of S100A₄ in PDL cells.

Key words: Periodontal ligament cell, S100A₄, RNA interference, Gene expression