The influence of polyvinylphosphonic acid on matrix metalloproteinases activities in dentin

doi:10.3877/cma.j.issn.1674-1366.2013.04.006

Abstract

Abstract:

Objective

The purpose of this study was to test the effect of polyvinylphosphonic acid （PVPA） which is used in biomimetic remineralization on dentin matrix metalloproteinases-2 （MMP-2）， MMP-8， MMP-9 activated by etching treatment.

Methods

In this study， 50 extracted non-caries human third molars were used to be pulverized and then divided into 8 groups as follows： negative control group only treated with 37% H₃PO₄； experimental groups： incubated in 100，200，500，1000 or 3000 μg/ml of PVPA after treated with 37% H₃PO₄， respectively； positive control groups： incubated in 0.2% or 2% chlorhexidine after treated with 37% H₃PO₄. The total protein was extracted， and the activities of MMPs， MMP-2， MMP-8 and MMP-9 were determined.

Results

All tested PVPA concentrations were highly effective in inhibiting MMPs. All tested PVPA concentrations show potent inhibitory effect on MMP-2， MMP-8 and MMP-9 activity except the 100 μg/ml PVPA treated group on MMP-8 activity. And a higher concentration of PVPA caused an increase in inhibitory effect.

Conclusion

PVPA is relatively effective in inhibiting MMPs and the inhibitory effect enhance with increasing PVPA concentration.

Key words: Polyvinylphosphonic acid, Matrix metalloproteinases, Dentin

En-bao HE, Yan WANG, Lan-chen LIANG, Yan-lan WANG, Xue-feng LIN. The influence of polyvinylphosphonic acid on matrix metalloproteinases activities in dentin[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2013, 07(04): 288-292.

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https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2013/V07/I04/288

Figures/Tables 4

References 22

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