The effects of simulating static magnetic fields of magnetic attachments on the TGF-β1 mRNA of osteoblasts

doi:10.3877/cma.j.issn.1674-1366.2012.04.007

Abstract

Abstract:

Objective

To explore the effects of static magnetic fields （SMF） of dental magnetic attachment system with different intensities on the expression of TGF-β1 mRNA of rat osteoblasts.

Methods

The cultured rat osteoblasts were exposed continuously to 12.5 mT， 125 mT， and 250 mT SMF for 0.5， 1， 3， 5， and 7 days， respectively. While， controls were also set up free of SMF. Realtime quantitative PCR was employed to detect the gene expression of cellular TGF-β1 mRNA and the statistical analysis was performed.

Results

Osteoblasts exposed to 12.5 mT SMF for 0.5， 1， and 3 days exhibited an increase on cellular TGF-β1 mRNA expression， as compared to those of the control group（P＜0.05）. Moreover， after 0.5， 1， 3， 5， and 7 days SMF exposure the cellular TGF-β1 mRNA expression in 125 mT and 250 mT groups were significantly higher than those of the control group and the 12.5 mT group （P＜0.05）. It showed a dose-effect relationship between the magnetic intensity and the expression of cellular TGF-β1 mRNA.

Conclusions

Under this experimental condition， the static magnetic fields of magnetic attachments can stimulate the expression of cellular TGF-β1 mRNA in rat osteoblasts， as to enchance the differentiation of osteoblasts.

Key words: Magnetic attachments, Static magnetic fields, Osteoblasts, Transforming growth factor

Yu ZHAO, Bing-yan LI, Ke ZHAO, Yong-lie CHAO. The effects of simulating static magnetic fields of magnetic attachments on the TGF-β1 mRNA of osteoblasts[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 6(04): 342-347.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2012.04.007

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2012/V6/I04/342

Figures/Tables 12

References 14

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基因		序列（5'-3'）
TGF-β1	F	ACTACTGCTTCAGCTCCACA
	TM	CCAAGGGCTACCATGCCAAC
	R	GTGTCCAGGCTCCAAATGT
GAPDH	F	TGGGTGTGAACCACGAGAA
	TM	CTGCACCACCAACTGCTTAGC
	R	GGCATGGACTGTGGTCATGA

基因		序列（5'-3'）
TGF-β1	F	ACTACTGCTTCAGCTCCACA
	TM	CCAAGGGCTACCATGCCAAC
	R	GTGTCCAGGCTCCAAATGT
GAPDH	F	TGGGTGTGAACCACGAGAA
	TM	CTGCACCACCAACTGCTTAGC
	R	GGCATGGACTGTGGTCATGA

组别	0.5 d	1 d	3 d	5 d	7 d	P
对照组	0.935±0.065	0.229±0.023	0.165±0.012	0.169±0.023	0.114±0.012	0.001
12.5 mT组	1.417±0.098^a	0.759±0.052^a	0.268±0.018^a	0.182±0.019	0.134±0.009	0.000
125 mT组	2.200±0.235^ab	1.423±0.197^ab	0.467±0.032^ab	0.354±0.025^ab	0.306±0.074^ab	0.001
250 mT组	2.833±0.196^abc	2.003±0.139^abc	0.481±0.067^ab	0.356±0.049^ab	0.359±0.061^ab	0.002
P	0.000	0.001	0.000	0.002	0.000	0.000^c

组别	0.5 d	1 d	3 d	5 d	7 d	P
对照组	0.935±0.065	0.229±0.023	0.165±0.012	0.169±0.023	0.114±0.012	0.001
12.5 mT组	1.417±0.098^a	0.759±0.052^a	0.268±0.018^a	0.182±0.019	0.134±0.009	0.000
125 mT组	2.200±0.235^ab	1.423±0.197^ab	0.467±0.032^ab	0.354±0.025^ab	0.306±0.074^ab	0.001
250 mT组	2.833±0.196^abc	2.003±0.139^abc	0.481±0.067^ab	0.356±0.049^ab	0.359±0.061^ab	0.002
P	0.000	0.001	0.000	0.002	0.000	0.000^c

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