Abstract:
Objective
To inverstigate the effect of curcumin on cell morphology of human tongue carcinoma cell line Tca8113.
Methods
Human tongue carcinoma cell line Tca8113 was incubated with different concentration of curcumin.Cell growth was observed by inverted microscope.Morphological features of apoptotic cell induced by curcumin were observed by Acridine orange staining and transmission electronic microscope （TEM）.
Results
Administration of 30， 40， 50 μmol/L curcumin for 24 h could significantly inhibit the growth of human tongue carcinoma cell line Tca8113.Cells treated with curcumin became smaller， rounded and then detached.LSCM found apoptotic cells with three conspicuous morphologic changes of the apoptotic cell nucleus， such as condensed nucleus of centrality and eccentricity and apoptotic bodies.Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic Tca8113 cells observed under TEM.
Conclusions
Curcumin can change the cell morphologic of human tongue carcinoma cell line Tca8113 and induce apoptosis.
Key words:
Curcumin,
Carcinoma, squamous cell,
Apoptosis
Zhi-jia YE, Jian-chang SHU, Hai-yan ZHU, Hai-en WU, Liang DENG. The effect of curcumin on human tongue carcinoma cell line Tca8113 about the cell morphologic[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2010, 4(03): 250-253.