Preliminarily study of PHBV barrier membrane for guided bone regeneration

doi:10.3877 / cma.j.issn.1674-1366.2009-03-008

Abstract

Abstract:

Objective

To study the biocompatibility of poly （hydroxybutyratehydroxyvalerate）（PHBV）， a novel biopolymer membrane， and evaluate its efficiency of promoting bone regeneration in vivo.

Methods

Cell relative growth rate （RGR） was measured by MTT method to quantitatively evaluate the cytotoxicity of the biomaterials. Eight healthy dogs were used as experimental animals. Four bone defects were made on bilateral tibias in each dog.The PHBV-membrane-guided bone generation effect was assessed by histological observation.

Results

The toxicity gradation of PHBV membrane ranked from grade 0 to grade 1； new bone regeneration was observed in the defects covered with PHBV membranes at 2^nd week post-surgery.The defects covered with PHBV membranes were filled with mature bone at 12^th week postsurgery. The bone rehabilitation in experimental groups was earlier and better than that in control group.

Conclusion

PHBV membrane， which has no cytotoxicity to MSCs in a wide range of extract concentration， may be applied as a promising biopolymer membrane for guided bone regeneration.

Key words: Poly （hydroxybutyrate-hydroxyvalerate）, Bone marrow stromal cells, Biocompati-bility, Bone defect, Guided bone regeneration

Jianhong CHEN, Qian TANG, Jian WU, Huang-you LIANG, Xiong-jun XU, Xiao-feng CHEN, Gang WU. Preliminarily study of PHBV barrier membrane for guided bone regeneration[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2009, 3(03): 280-286.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877 / cma.j.issn.1674-1366.2009-03-008

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2009/V3/I03/280

Figures/Tables 8

组别	培养时间（d）	A值（ $\bar{x}$ ±s）	细胞相对增殖率（%）	毒性分级
A	1	0.397±0.065	99.37	1
	3	0.409±0.102	98.26	1
	5	0.794±0.125	98.91	1
B	1	0.400±0.065	100.06	0
	3	0.410±0.082	98.50	1
	5	0.796±0.073	99.19	1
C	1	0.400±0.045	100.06	0
	3	0.415±0.072	99.76	1
	5	0.796±0.079	99.19	1
D	1	0.400±0.042	100.00	0
	3	0.416±0.068	99.88	1
	5	0.801±0.081	99.84	1
E	1	0.400±0.043
	3	0.418±0.045
	5	0.805±0.053

组别	培养时间（d）	A值（ $\bar{x}$ ±s）	细胞相对增殖率（%）	毒性分级
A	1	0.397±0.065	99.37	1
	3	0.409±0.102	98.26	1
	5	0.794±0.125	98.91	1
B	1	0.400±0.065	100.06	0
	3	0.410±0.082	98.50	1
	5	0.796±0.073	99.19	1
C	1	0.400±0.045	100.06	0
	3	0.415±0.072	99.76	1
	5	0.796±0.079	99.19	1
D	1	0.400±0.042	100.00	0
	3	0.416±0.068	99.88	1
	5	0.801±0.081	99.84	1
E	1	0.400±0.043
	3	0.418±0.045
	5	0.805±0.053

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