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中华口腔医学研究杂志(电子版) ›› 2016, Vol. 10 ›› Issue (04) : 238 -243. doi: 10.3877/cma.j.issn.1674-1366.2016.04.002

所属专题: 文献

基础研究

变异链球菌转肽酶A基因型与乳牙高龋的相关性
于丽霞1, 庄沛林2, 林焕彩1,()   
  1. 1. 510055 广州,中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室
    2. 510055 广州,中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室;510120 广州,中山大学孙逸仙纪念医院口腔科
  • 收稿日期:2016-06-14 出版日期:2016-08-01
  • 通信作者: 林焕彩
  • 基金资助:
    国家自然科学基金(81271123)

The correlation between the sortaseA genotype of Streptococcus mutans and high-severity childhood caries

Lixia Yu1, Peilin Zhuang2, Huancai Lin1,()   

  1. 1. Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
    2. Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China; Department of Stomatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
  • Received:2016-06-14 Published:2016-08-01
  • Corresponding author: Huancai Lin
  • About author:
    Corresponding author: Lin Huancai, Email:
引用本文:

于丽霞, 庄沛林, 林焕彩. 变异链球菌转肽酶A基因型与乳牙高龋的相关性[J/OL]. 中华口腔医学研究杂志(电子版), 2016, 10(04): 238-243.

Lixia Yu, Peilin Zhuang, Huancai Lin. The correlation between the sortaseA genotype of Streptococcus mutans and high-severity childhood caries[J/OL]. Chinese Journal of Stomatological Research(Electronic Edition), 2016, 10(04): 238-243.

目的

在控制乳牙龋危险指标的前提下,探讨乳牙高龋与变异链球菌(简称变链菌)转肽酶A(srtA)基因型的关系。

方法

采用ABI Variant Reporter软件对121株分离于高龋儿童的变链菌和121株分离于无龋儿童的变链菌srtA基因测序结果进行基因型分析。采用单因素分析方法分析srtA基因型数量、srtA各基因型以及社会、行为变量在两组中的频率分布情况,采用多因素Logistic回归方法控制混杂因素,分析变链菌srtA基因型与乳牙高龋的相关性。

结果

共发现15种基因型,其中无龋组中9种,高龋组中11种。3A基因型在无龋组中的突变率高于高龋组(P= 0.033),3E基因型在高龋组中的突变率高于无龋组(P= 0.037)。多因素分析结果表明:高月龄、低出生体重、过长的母乳喂养时间、高固体糖摄入频率、较高的可视菌斑百分率、较少的srtA 3A基因型与乳牙高龋相关。

结论

在有变链菌定植的儿童中,变链菌srtA 3A基因型与乳牙龋易感性相关。此外,月龄、出生体重、母乳持续喂养时间、固体糖摄入频率、可视菌斑等因素也参与早期儿童龋的发生、发展。

Objectives

To make a primary research on the relationship between genotypes of sortase A gene (srtA) of Streptococcus mutans (S.mutans) and high-severity childhood caries on the base of existenceof no other factors related to childhood caries.

Methods

The ABI Variant Reporter software was used to analyze the genotypes of the srtA gene of 121 S.mutans strains isolated from the high-severity caries children and 121 S.mutans strains isolated from the caries-free children. Bivariate analysis was used to analyze the srtA genotype distribution and the frequencies of social and behavioural variants between groups. Multiple logistic regression models were used to assess the relationship between the srtA genotype and childhood caries after controlling confounding factors.

Results

A total of 15 genotypes were found: 9 in caries free group and 11 in high-severity caries group. The frequency of 3A genotype was significantly higher in caries free group than high-severity caries group (P= 0.033) . The frequency of 3E genotype was significantly higher in high-severity caries group than caries free group (P= 0.037) . The best-fitting model showed that older age (months) , low weight at birth, high frequencies of solid sugar consumption, prolonged breastfeeding, a high proportion of visible plaque, and 3A genotype of the srtA gene of S.mutans were associated with high-severity caries in children.

Conclusions

The present study suggested that the 3A genotype of the srtA gene of S.mutans may correlate with caries susceptibility in children. In addition, age, weight at birth, duration of breastfeeding, solid sugar consumption, and poor oral hygiene contributed to primary childhood caries.

表1 PCR扩增引物及产物片段大小
表2 研究对象的主要变量及其分类或记分
表3 变异链球菌srtA功能区基因型命名及碱基突变情况
表4 变异链球菌srtA功能区基因型与乳牙高龋的相关性分析(n= 121)
图1 测序样本序列比对图
表5 变异链球菌srtA功能区基因型与乳牙高龋相关性的Logistic回归分析
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