Research progress and prospects of long non-coding RNA regulating osteogenic differentiation

doi:10.3877/cma.j.issn.1674-1366.2020.05.001

Abstract

Abstract:

Defect and abnormity of craniofacial bones not only cause corresponding functional disorders, but also affect the outlooking and mental health of patients. The osteogenic differentiation from mesenchymal stem cells to osteoblast lineage is not only the foundation of osteogenesis, but also one of the key factors maintaining bone homeostasis. Abnormal osteogenic differentiation is strongly relative to many bone diseases and has side effects on bone regeneration. As a newly discovered regulatory factor, long non-coding RNA plays an important role in osteogenic differentiation. This article focused on the long non-coding RNA that played important roles in regulating osteogenic differentiation, discussing the mechanisms, existing problems as well as prospects.

Key words: RNA, long noncoding, Mesenchymal stem cell, Osteogenic differentiation, Osteogenesis, Molecular mechanism

Runze Li, Jianhan Ren, Delan Huang, Haotian Luo, Chen Zhou, Weicai Wang. Research progress and prospects of long non-coding RNA regulating osteogenic differentiation[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2020, 14(05): 271-279.

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References 70

[1]	Lee H, Zhang Z, Krause HM. Long Noncoding RNAs and Repetitive Elements：Junk or Intimate Evolutionary Partners？[J]. Trends Genet，2019，35(12): 892-902. DOI: 10.1016/j.tig.2019.09.006.
[2]	Uszczynska-Ratajczak B, Lagarde J, Frankish A，et al. Towards a complete map of the human long non-coding RNA transcriptome[J]. Nat Rev Genet，2018，19(9): 535-548. DOI: 10.1038/s41576-018-0017-y.
[3]	Yao RW, Wang Y, Chen LL. Cellular functions of long noncoding RNAs[J]. Nat Cell Biol，2019，21(5): 542-551. DOI: 10.1038/s41556-019-0311-8.
[4]	Sun Q, Hao Q, Prasanth KV. Nuclear Long Noncoding RNAs：Key Regulators of Gene Expression[J]. Trends Genet，2018，34(2): 142-157. DOI: 10.1016/j.tig.2017.11.005.
[5]	Wu H, Yang L, Chen LL. The Diversity of Long Noncoding RNAs and Their Generation[J]. Trends Genet，2017，33(8): 540-552. DOI: 10.1016/j.tig.2017.05.004.
[6]	Wang KC, Chang HY. Molecular Mechanisms of Long Noncoding RNAs[J]. Mol Cell，2011，43(6): 904-914. DOI: 10.1016/j.molcel.2011.08.018.
[7]	Marchese FP, Raimondi I, Huarte M. The multidimensional mechanisms of long noncoding RNA function[J]. Genome Biol，2017，18(1): 206. DOI: 10.1186/s13059-017-1348-2.
[8]	Kaikkonen MU, Adelman K. Emerging Roles of Non-Coding RNA Transcription[J]. Trends Biochem Sci，2018，43(9): 654-667. DOI: 10.1016/j.tibs.2018.06.002.
[9]	Huang JZ, Chen M, Chen D，et al. A Peptide Encoded by a Putative LncRNA HOXB-AS3 Suppresses Colon Cancer Growth[J]. Molecular Cell，2017，68(1): 171-184.e6. DOI: 10.1016/j.molcel.2017.09.015.
[10]	Hankenson KD, Gagne K, Shaughnessy M. Extracellular signaling molecules to promote fracture healing and bone regeneration[J]. Adv Drug Deliv Rev，2015，94: 3-12. DOI: 10.1016/j.addr.2015.09.008.
[11]	Murshed M. Mechanism of Bone Mineralization[J]. Cold Spring Harbor Perspectives in Medicine，2018，8(12): a031229. DOI: 10.1101/cshperspect.a031229.
[12]	Lopes D, Martins-Cruz C, Oliveira MB，et al. Bone physiology as inspiration for tissue regenerative therapies[J]. Biomaterials，2018，185: 240-275. DOI: 10.1016/j.biomaterials.2018.09.028.
[13]	Wu M, Wang Y, Shao JZ，et al. Cbfβ Governs osteoblast-adipocyte lineage commitment through enhancing β-catenin signaling and suppressing adipogenesis gene expression[J]. Proc Natl Acad Sci U S A，2017，114(38): 10119-10124. DOI: 10.1073/pnas.1619294114.
[14]	Vimalraj S, Arumugam B, Miranda PJ，et al. Runx2：Structure，function，and phosphorylation in osteoblast differentiation[J]. Int J Biol Macromol，2015，78: 202-208. DOI: 10.1016/j.ijbiomac.2015.04.008.
[15]	An J, Yang H, Zhang Q，et al. Natural products for treatment of osteoporosis：The effects and mechanisms on promoting osteoblast-mediated bone formation[J]. Life Sci，2016，147: 46-58. DOI: 10.1016/j.lfs.2016.01.024.
[16]	Sinha KM, Zhou X. Genetic and molecular control of osterix in skeletal formation[J]. J Cell Biochem，2013，114(5): 975-984. DOI: 10.1002/jcb.24439.
[17]	Majidinia M, Sadeghpour A, Yousefi B. The Roles of Signaling Pathways in Bone Repair and Regeneration[J]. J Cell Physiol，2018，233(4): 2937-2948. DOI: 10.1002/jcp.26042.
[18]	Wang J, Liu S, Li J，et al. Roles for miRNAs in osteogenic differentiation of bone marrow mesenchymal stem cells[J]. Stem Cell Res Ther，2019，10(1): 197. DOI: 10.1186/s13287-019-1309-7.
[19]	Zhang W, Dong R, Diao S，et al. Differential long noncoding RNA/mRNA expression profiling and functional network analysis during osteogenic differentiation of human bone marrow mesenchymal stem cells[J]. Stem Cell Res Ther，2017，8(1): 30. DOI: 10.1186/s13287-017-0485-6.
[20]	Yu L, Qu H, Yu Y，et al. LncRNA-PCAT1 targeting miR-145-5p promotes TLR4-associated osteogenic differentiation of adipose-derived stem cells[J]. J Cell Mol Med，2018，22(12): 6134-6147. DOI: 10.1111/jcmm.13892.
[21]	Tang S, Xie Z, Wang P，et al. LncRNA-OG Promotes the Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells Under the Regulation of HnRNPK[J]. Stem Cells，2019，37(2): 270-283. DOI: 10.1002/stem.2937.
[22]	Wang J, Miao J, Meng X，et al. Expression of long non-coding RNAs in human bone marrow mesenchymal stem cells co-cultured with human amnion-derived mesenchymal stem cells[J]. Molecular Medicine Reports，2017，16(5): 6683-6689. DOI: 10.3892/mmr.2017.7465.
[23]	Qiu X, Jia B, Sun X，et al. The Critical Role of Long Noncoding RNA in Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells[J]. Biomed Res Int，2017，2017: 5045827. DOI: 10.1155/2017/5045827.
[24]	Cui Y, Lu S, Tan H，et al. Silencing of Long Non-Coding RNA NONHSAT009968 Ameliorates the Staphylococcal Protein A-Inhibited Osteogenic Differentiation in Human Bone Mesenchymal Stem Cells[J]. Cell Physiol Biochem，2016，39(4): 1347-1359. DOI: 10.1159/000447839.
[25]	Wu R, Ruan J, Sun Y，et al. Long non-coding RNA HIF1A-AS2 facilitates adipose-derived stem cells（ASCs）osteogenic differentiation through miR-665/IL6 axis via PI3K/Akt signaling pathway[J]. Stem Cell Res Ther，2018，9(1): 348. DOI: 10.1186/s13287-018-1082-z.
[26]	Huang G, Kang Y, Huang Z，et al. Identification and Characterization of Long Non-Coding RNAs in Osteogenic Differentiation of Human Adipose-Derived Stem Cells[J]. Cell Physiol Biochem，2017，42(3): 1037-1050. DOI: 10.1159/000478751.
[27]	Gu X, Li M, Jin Y，et al. Identification and integrated analysis of differentially expressed lncRNAs and circRNAs reveal the potential ceRNA networks during PDLSC osteogenic differentiation[J]. BMC Genet，2017，18: 100. DOI: 10.1186/s12863-017-0569-4.
[28]	Sun X, Yuan Y, Xiao Y，et al. Long non-coding RNA，Bmcob，regulates osteoblastic differentiation of bone marrow mesenchymal stem cells[J]. Biochem Biophys Res Commun，2018，506(3): 536-542. DOI: 10.1016/j.bbrc.2018.09.142.
[29]	Wang Y, Liu W, Liu Y，et al. Long noncoding RNA H19 mediates LCoR to impact the osteogenic and adipogenic differentiation of MBMSCs in mice through sponging MiR-188[J]. J Cell Physiol，2018，233(9): 7435-7446. DOI: 10.1002/jcp.26589.
[30]	Hou Q, Huang Y, Liu Y，et al. Profiling the miRNA-mRNA-lncRNA interaction network in MSC osteoblast differentiation induced by（+）-cholesten-3-one[J]. BMC Genomics，2018，19: 783. DOI: 10.1186/s12864-018-5155-2.
[31]	Song WQ, Gu WQ, Qian YB，et al. Identification of long non-coding RNA involved in osteogenic differentiation from mesenchymal stem cells using RNA-Seq data[J]. Genet Mol Res，2015，14(4): 18268-18279. DOI: 10.4238/2015.December.23.14.
[32]	Kim M, Yu Y, Moon JH，et al. Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse[J]. Int J Genomics，2018: 7691794. DOI: 10.1155/2018/7691794.
[33]	Zuo C, Wang Z, Lu H，et al. Expression Profiling of LncRNAs in C3H10T1/2 Mesenchymal Stem Cells Undergoing Early Osteoblast Differentiation[J]. Mol Med Rep，2013，8(2): 463-467. DOI: 10.3892/mmr.2013.1540.
[34]	Cavalli G, Heard E. Advances in epigenetics link genetics to the environment and disease[J]. Nature，2019，571(7766): 489-499. DOI: 10.1038/s41586-019-1411-0.
[35]	He S, Yang S, Zhang Y，et al. LncRNA ODIR1 Inhibits Osteogenic Differentiation of HUC-MSCs through the FBXO25/H2BK120ub/H3K4me3/OSX Axis[J]. Cell Death Dis，2019，10(12): 947. DOI: 10.1038/s41419-019-2148-2.
[36]	Fu L, Peng S, Wu W，et al. LncRNA HOTAIRM1 promotes osteogenesis by controlling JNK/AP-1 signalling-mediated RUNX2 expression[J]. J Cell Mol Med，2019，23(11): 7517-7524. DOI: 10.1111/jcmm.14620.
[37]	Chen Z, Zheng J, Hong H，et al. LncRNA HOTAIRM1 promotes osteogenesis of HDFSCs by epigenetically regulating HOXA2 via DNMT1 in vitro[J]. J Cell Physiol，2020: 1-13. DOI: 10.1002/jcp.29695.
[38]	Batista PJ, Chang HY. Long noncoding RNAs：cellular address codes in development and disease[J]. Cell，2013，152(6): 1298-1307. DOI: 10.1016/j.cell.2013.02.012.
[39]	Zhang W, Chen L, Wu J，et al. Long noncoding RNA TUG1 inhibits osteogenesis of bone marrow mesenchymal stem cells via Smad5 after irradiation[J]. Theranostics，2019，9(8): 2198-2208. DOI: 10.7150/thno.30798.
[40]	Li D, Yu K, Xiao T，et al. LOC103691336/MiR-138-5p/BMPR2 axis modulates Mg-mediated osteogenic differentiation in rat femoral fracture model and rat primary bone marrow stromal cells[J]. J Cell Physiol，2019，234(11): 21316-21330. DOI: 10.1002/jcp.28736.
[41]	Li B, Xu H, Han H，et al. Exosome-mediated transfer of lncRUNX2-AS1 from multiple myeloma cells to MSCs contributes to osteogenesis[J]. Oncogene，2018，37(41): 5508-5519. DOI: 10.1038/s41388-018-0359-0.
[42]	Li B, Han H, Song S，et al. HOXC10 Regulates Osteogenesis of Mesenchymal Stromal Cells Through Interaction with Its Natural Antisense Transcript LncHOXC-AS3[J]. Stem Cells，2019，37(2): 247-256. DOI: 10.1002/stem.2925.
[43]	Feng L, Shi L, Lu Y，et al. Linc-ROR Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Functioning as a Competing Endogenous RNA for miR-138 and miR-145[J]. Mol Ther Nucleic Acids，2018，11: 345-353. DOI: 10.1016/j.omtn.2018.03.004.
[44]	Shang G, Wang Y, Xu Y，et al. Long non-coding RNA TCONS_00041960 enhances osteogenesis and inhibits adipogenesis of rat bone marrow mesenchymal stem cell by targeting miR-204-5p and miR-125a-3p[J]. J Cell Physiol，2018，233(8): 6041-6051. DOI: 10.1002/jcp.26424.
[45]	Huang P, Yan R, Zhang X，et al. Activating Wnt/β-catenin signaling pathway for disease therapy：Challenges and opportunities[J]. Pharmacol Ther，2019，196: 79-90. DOI: 10.1016/j.pharmthera.2018.11.008.
[46]	Gao X, Ge J, Li W，et al. LncRNA KCNQ1OT1 promotes osteogenic differentiation to relieve osteolysis via Wnt/β-catenin activation[J/OL]. Cell Biosci，2018，8: 19. DOI: 10.1186/s13578-018-0216-4.
[47]	Xu Y, Qin W, Guo D，et al. LncRNA-TWIST1 Promoted Osteogenic Differentiation Both in PPDLSCs and in HPDLSCs by Inhibiting TWIST1 Expression[J]. Biomed Res Int，2019，2019: 8735952. DOI: 10.1155/2019/8735952.
[48]	Shi Y, Massagué J. Mechanisms of TGF-β signaling from cell membrane to the nucleus[J]. Cell，2003，113(6): 685-700. DOI: 10.1016/S0092-8674(03)00432-X
[49]	Budi EH, Duan D, Derynck R. Transforming Growth Factor-β Receptors and Smads：Regulatory Complexity and Functional Versatility[J]. Trends Cell Biol，2017，27(9): 658-672. DOI: 10.1016/j.tcb.2017.04.005.
[50]	Li D, Yu K, Xiao T，et al. LOC103691336/MiR-138-5p/BMPR2 axis modulates Mg-mediated osteogenic differentiation in rat femoral fracture model and rat primary bone marrow stromal cells[J]. J Cell Physiol，2019，234(11): 21316-21330. DOI: 10.1002/jcp.28736.
[51]	Mulero MC, Wang VYF, Huxford T，et al. Genome reading by the NF-ΚB transcription factors[J]. Nucleic Acids Research，2019，47(19): 9967-9989. DOI: 10.1093/nar/gkz739.
[52]	Zhu X, Yu J, Du J，et al. LncRNA HOXA-AS2 positively regulates osteogenesis of mesenchymal stem cells through inactivating NF-κB signalling[J]. J Cell Mol Med，2019，23(2): 1325-1332. DOI: 10.1111/jcmm.14034.
[53]	Zhang J, Tao Z, Wang Y. Long non-coding RNA DANCR regulates the proliferation and osteogenic differentiation of human bone-derived marrow mesenchymal stem cells via the p38 MAPK pathway[J]. Int J Mol Med，2018，41(1): 213-219. DOI: 10.3892/ijmm.2017.3215.
[54]	Zhang Y, Cao X, Li P，et al. LncRNA NKILA integrates RXFP1/AKT and NF-κB signalling to regulate osteogenesis of mesenchymal stem cells[J]. J Cell Mol Med，2020，24(1): 521-529. DOI: 10.1111/jcmm.14759.
[55]	Huang Y, Han Y, Guo R，et al. Long non-coding RNA FER1L4 promotes osteogenic differentiation of human periodontal ligament stromal cells via miR-874-3p and vascular endothelial growth factor A[J]. Stem Cell Res Ther，2020，11(1): 5. DOI: 10.1186/s13287-019-1519-z.
[56]	Cai WL, Zeng W, Liu HH，et al. LncRNA LINC00707 promotes osteogenic differentiation of hBMSCs through the Wnt/β-catenin pathway activated by LINC00707/miR-145/LRP5 axis[J]. Eur Rev Med Pharmacol Sci，2020，24(1): 18-28. DOI: 10.26355/eurrev_202001_19891.
[57]	Zhang HL, Du XY, Dong QR. LncRNA XIXT promotes osteogenic differentiation of bone mesenchymal stem cells and alleviates osteoporosis progression by targeting miRNA-30a-5p[J]. Eur Rev Med Pharmacol Sci，2019，23(20): 8721-8729. DOI: 10.26355/eurrev_201910_19266.
[58]	Zhang N, Hu X, He S，et al. LncRNA MSC-AS1 promotes osteogenic differentiation and alleviates osteoporosis through sponging microRNA-140-5p to upregulate BMP2[J]. Biochem Biophys Res Commun，2019，519(4): 790-796. DOI: 10.1016/j.bbrc.2019.09.058.
[59]	Jiang Y, Wu W, Jiao G，et al. LncRNA SNHG1 modulates p38 MAPK pathway through Nedd4 and thus inhibits osteogenic differentiation of bone marrow mesenchymal stem cells[J]. Life Sci，2019，228: 208-214. DOI: 10.1016/j.lfs.2019.05.002.
[60]	Wu J, Zhao J, Sun L，et al. Long non-coding RNA H19 mediates mechanical tension-induced osteogenesis of bone marrow mesenchymal stem cells via FAK by sponging miR-138[J]. Bone，2018，108: 62-70. DOI: 10.1016/j.bone.2017.12.013.
[61]	Wang Q, Li Y, Zhang Y，et al. LncRNA MEG3 inhibited osteogenic differentiation of bone marrow mesenchymal stem cells from postmenopausal osteoporosis by targeting miR-133a-3p[J]. Biomed Pharmacother，2017，89: 1178-1186. DOI: 10.1016/j.biopha.2017.02.090.
[62]	Deng L, Hong H, Zhang X，et al. Down-regulated lncRNA MEG3 promotes osteogenic differentiation of human dental follicle stem cells by epigenetically regulating Wnt pathway[J]. Biochem Biophys Res Commun，2018，503(3): 2061-2067. DOI: 10.1016/j.bbrc.2018.07.160.
[63]	He Q, Yang S, Gu X，et al. Long noncoding RNA TUG1 facilitates osteogenic differentiation of periodontal ligament stem cells via interacting with Lin28A[J]. Cell Death & Disease，2018，9(5): 455. DOI: 10.1038/s41419-018-0484-2.
[64]	Gao Y, Xiao F, Wang C，et al. Long noncoding RNA MALAT1 promotes osterix expression to regulate osteogenic differentiation by targeting miRNA-143 in human bone marrow-derived mesenchymal stem cells[J]. J Cell Biochem，2018，119(8): 6986-6996. DOI: 10.1002/jcb.26907.
[65]	Yi J, Liu D, Xiao J. LncRNA MALAT1 sponges miR-30 to promote osteoblast differentiation of adipose-derived mesenchymal stem cells by promotion of Runx2 expression[J]. Cell Tissue Res，2019，376(1): 113-121. DOI: 10.1007/s00441-018-2963-2.
[66]	Yang X, Yang J, Lei P，et al. LncRNA MALAT1 shuttled by bone marrow-derived mesenchymal stem cells-secreted exosomes alleviates osteoporosis through mediating microRNA-34c/SATB2 axis[J]. Aging（Albany NY），2019，11(20): 8777-8791. DOI: 10.18632/aging.102264.
[67]	Cui Y, Fu S, Sun D，et al. EPC-derived exosomes promote osteoclastogenesis through LncRNA-MALAT1[J]. J Cell Mol Med，2019，23(6): 3843-3854. DOI: 10.1111/jcmm.14228.
[68]	Sun Z, Yang S, Zhou Q，et al. Emerging role of exosome-derived long non-coding RNAs in tumor microenvironment[J]. Molecular Cancer，2018，17(1): 82. DOI: 10.1186/s12943-018-0831-z.
[69]	Li Y, Glass Z, Huang M，et al. Ex vivo cell-based CRISPR/Cas9 genome editing for therapeutic applications[J]. Biomaterials，2020，234: 119711. DOI: 10.1016/j.biomaterials.2019.119711.
[70]	Ma C, Kuzma ML, Bai X，et al. Biomaterial-Based Metabolic Regulation in Regenerative Engineering[J]. Advanced Sci，2019，6(19): 1900819. DOI: 10.1002/advs.201900819.

研究模型	LncRNA表达谱	注释	参考文献	年份
hBMSC	785个上调；623个下调	成骨诱导分化-未成骨诱导分化对比	[19]	2017
hBMSC	318个上调；732个下调	成骨诱导分化-未成骨诱导分化对比	[21]	2019
hBMSC	208个上调；131个下调	与hAMSC共培养后成骨诱导分化-未共培养后成骨诱导分化对比	[22]	2017
hBMSC	433个上调，233个下调	成骨诱导分化-未成骨诱导分化对比	[23]	2017
hBMSC	1392个上调，641个下调	经葡萄球菌A蛋白(SpA)处理后成骨诱导分化-未处理后成骨诱导分化对比	[24]	2016
hASC	478个上调；507下调	成骨诱导分化-未成骨诱导分化对比	[25]	2018
hASC	1460个上调，1112个下调	成骨诱导分化-未成骨诱导分化对比	[26]	2017
hPDLSC	777个上调；183个下调	成骨诱导分化-未成骨诱导分化对比	[27]	2017
mBMSC	16个上调；24个下调	成骨诱导分化-未成骨诱导分化对比	[28]	2018
mBMSC	140个上调；151个下调	成骨诱导分化-未成骨诱导分化对比	[29]	2018
rMSC	281个上调；14个下调	经4-胆甾烯-3-酮诱导成骨向分化-未经诱导对比	[30]	2018
iMSC	574个差异性表达	成骨诱导分化-未成骨诱导分化对比	[31]	2015
Mouse calvarial preosteoclast cells	3948个差异性表达	成骨诱导分化前期(2 ~ 8 d)与后期(10 ~ 18 d)对比	[32]	2018
C3H10T1/2 cells	59个上调；57个下调	骨形成蛋白2(BMP-2)处理前后对比	[33]	2013

研究模型	LncRNA表达谱	注释	参考文献	年份
hBMSC	785个上调；623个下调	成骨诱导分化-未成骨诱导分化对比	[19]	2017
hBMSC	318个上调；732个下调	成骨诱导分化-未成骨诱导分化对比	[21]	2019
hBMSC	208个上调；131个下调	与hAMSC共培养后成骨诱导分化-未共培养后成骨诱导分化对比	[22]	2017
hBMSC	433个上调，233个下调	成骨诱导分化-未成骨诱导分化对比	[23]	2017
hBMSC	1392个上调，641个下调	经葡萄球菌A蛋白(SpA)处理后成骨诱导分化-未处理后成骨诱导分化对比	[24]	2016
hASC	478个上调；507下调	成骨诱导分化-未成骨诱导分化对比	[25]	2018
hASC	1460个上调，1112个下调	成骨诱导分化-未成骨诱导分化对比	[26]	2017
hPDLSC	777个上调；183个下调	成骨诱导分化-未成骨诱导分化对比	[27]	2017
mBMSC	16个上调；24个下调	成骨诱导分化-未成骨诱导分化对比	[28]	2018
mBMSC	140个上调；151个下调	成骨诱导分化-未成骨诱导分化对比	[29]	2018
rMSC	281个上调；14个下调	经4-胆甾烯-3-酮诱导成骨向分化-未经诱导对比	[30]	2018
iMSC	574个差异性表达	成骨诱导分化-未成骨诱导分化对比	[31]	2015
Mouse calvarial preosteoclast cells	3948个差异性表达	成骨诱导分化前期(2 ~ 8 d)与后期(10 ~ 18 d)对比	[32]	2018
C3H10T1/2 cells	59个上调；57个下调	骨形成蛋白2(BMP-2)处理前后对比	[33]	2013

LncRNA	细胞模型	体内验证	调控机制	相关信号通路	作用效果	参考文献	年份
NKILA	hMenSC & hUC-MSC	无	NF-κB/HDAC2/RUNX2;RXFP1/Akt	NF-κB通路；PI3K-Akt通路	促成骨	[54]	2020
FER1L4	hPDLSC	有	miR-874-3p/VEGFA	未明确	促成骨	[55]	2020
LINC00707	hBMSC	无	miR-145/LRP5/β-catenin	Wnt/β-catenin通路	促成骨	[56]	2020
HOXA-AS2	hUC-MSC	无	NF-κB/HDAC2/SP7	NF-κB通路	促成骨	[52]	2019
XIXT	hBMSC	无	miR-30a-5p/RUNX2	未明确	促成骨	[57]	2019
MSC-AS1	BMSC	无	miR-140-5p/BMP2/Smad	BMP通路	促成骨	[58]	2019
AK045490	MC3T3-E1	有	β-Catenin/TCF1/Runx2	Wnt/β-Catenin通路	抑制成骨	[40]	2019
LncRNA-OG	hBMSC	有	hnRNPK/LncRNA-OG/BMP family	BMP通路	促成骨	[21]	2019
HOXC-AS3	hBMSC;U266 cell line	有	提高HOXC10 mRNA稳定性	未明确	抑制成骨	[42]	2019
LOC103691336	rBMSC	有	miR-138-5p/BMP2	BMP通路	促成骨	[50]	2019
ODIR1	hUC-MSC	有	CUL3/FBXO25/OSX	未明确	抑制成骨	[35]	2019
HOTAIRM1	hMenSC;hUC-MSC	无	JNK/c-Jun/P300/RUNX2	JNK/AP-1通路	抑制成骨	[36]	2019
?	hDFSC	无	DNMT1/HOXA2	未明确	促成骨	[37]	2020
SNHG1	mBMSC	无	Nedd4/p38	P38 MAPK通路	抑制成骨	[59]	2019
PCAT1	hASC	无	miR-145-5p/TLR4	TLR通路	促成骨	[20]	2018
HIF1A-AS2	hADSC	无	miR-665/IL6/PI3K/Akt	PI3K/Akt通路	促成骨	[25]	2018
RUNX2-AS1	hBMSC	有	抑制RUNX2 pre-mRNA选择性剪接	未明确	抑制成骨	[41]	2018
DANCR	hBMSC	无	抑制p38磷酸化	P38 MAPK通路	抑制成骨	[53]	2018
H19	mBMSC	无	miR-188/LCoR	不明	促成骨	[29]	2018
?	hBMSC	无	miR-138/PTK2	PTK通路	促成骨	[60]	2018
KCNQ1OT1	mMSC	无	与Wnt/β-catenin通路相关	Wnt/β-catenin通路	促成骨	[46]	2018
Linc-ROR	hBMSC	无	miR-138 & miR-145/ZEB2/β-catenin	Wnt/β-catenin通路	促成骨	[43]	2018
MEG3	hBMSC	无	miR-133a-3p/SLC39A1	未明确	抑制成骨	[61]	2017
?	hDFSC	无	EZH2/β-catenin	Wnt/β-catenin通路	抑制成骨	[62]	2018
TUG1	hPDLSC	无	募集LIN28A	未明确	促成骨	[63]	2018
?	hBMSC	无	抑制p-SMAD5核转位	未明确	抑制成骨	[39]	2019
TCONS_00041960	rBMSC	无	miR-204-5p/RUNX2	未明确	促成骨	[56]	2020
?	hBMSC	无	miR-143/OSX	未明确	促成骨	[64]	2018
MALAT1	hASC	无	miR-30/RUNX2	未明确	促成骨	[65]	2019
?	hBMSC & hFOB1.19	有	miR-34c/SATB2	未明确	促成骨	[66]	2019

LncRNA	细胞模型	体内验证	调控机制	相关信号通路	作用效果	参考文献	年份
NKILA	hMenSC & hUC-MSC	无	NF-κB/HDAC2/RUNX2;RXFP1/Akt	NF-κB通路；PI3K-Akt通路	促成骨	[54]	2020
FER1L4	hPDLSC	有	miR-874-3p/VEGFA	未明确	促成骨	[55]	2020
LINC00707	hBMSC	无	miR-145/LRP5/β-catenin	Wnt/β-catenin通路	促成骨	[56]	2020
HOXA-AS2	hUC-MSC	无	NF-κB/HDAC2/SP7	NF-κB通路	促成骨	[52]	2019
XIXT	hBMSC	无	miR-30a-5p/RUNX2	未明确	促成骨	[57]	2019
MSC-AS1	BMSC	无	miR-140-5p/BMP2/Smad	BMP通路	促成骨	[58]	2019
AK045490	MC3T3-E1	有	β-Catenin/TCF1/Runx2	Wnt/β-Catenin通路	抑制成骨	[40]	2019
LncRNA-OG	hBMSC	有	hnRNPK/LncRNA-OG/BMP family	BMP通路	促成骨	[21]	2019
HOXC-AS3	hBMSC;U266 cell line	有	提高HOXC10 mRNA稳定性	未明确	抑制成骨	[42]	2019
LOC103691336	rBMSC	有	miR-138-5p/BMP2	BMP通路	促成骨	[50]	2019
ODIR1	hUC-MSC	有	CUL3/FBXO25/OSX	未明确	抑制成骨	[35]	2019
HOTAIRM1	hMenSC;hUC-MSC	无	JNK/c-Jun/P300/RUNX2	JNK/AP-1通路	抑制成骨	[36]	2019
?	hDFSC	无	DNMT1/HOXA2	未明确	促成骨	[37]	2020
SNHG1	mBMSC	无	Nedd4/p38	P38 MAPK通路	抑制成骨	[59]	2019
PCAT1	hASC	无	miR-145-5p/TLR4	TLR通路	促成骨	[20]	2018
HIF1A-AS2	hADSC	无	miR-665/IL6/PI3K/Akt	PI3K/Akt通路	促成骨	[25]	2018
RUNX2-AS1	hBMSC	有	抑制RUNX2 pre-mRNA选择性剪接	未明确	抑制成骨	[41]	2018
DANCR	hBMSC	无	抑制p38磷酸化	P38 MAPK通路	抑制成骨	[53]	2018
H19	mBMSC	无	miR-188/LCoR	不明	促成骨	[29]	2018
?	hBMSC	无	miR-138/PTK2	PTK通路	促成骨	[60]	2018
KCNQ1OT1	mMSC	无	与Wnt/β-catenin通路相关	Wnt/β-catenin通路	促成骨	[46]	2018
Linc-ROR	hBMSC	无	miR-138 & miR-145/ZEB2/β-catenin	Wnt/β-catenin通路	促成骨	[43]	2018
MEG3	hBMSC	无	miR-133a-3p/SLC39A1	未明确	抑制成骨	[61]	2017
?	hDFSC	无	EZH2/β-catenin	Wnt/β-catenin通路	抑制成骨	[62]	2018
TUG1	hPDLSC	无	募集LIN28A	未明确	促成骨	[63]	2018
?	hBMSC	无	抑制p-SMAD5核转位	未明确	抑制成骨	[39]	2019
TCONS_00041960	rBMSC	无	miR-204-5p/RUNX2	未明确	促成骨	[56]	2020
?	hBMSC	无	miR-143/OSX	未明确	促成骨	[64]	2018
MALAT1	hASC	无	miR-30/RUNX2	未明确	促成骨	[65]	2019
?	hBMSC & hFOB1.19	有	miR-34c/SATB2	未明确	促成骨	[66]	2019

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