The study of bone morphogenetic protein 2 immobilization on titanium surface with polymer electrostatic interaction

doi:10.3877/cma.j.issn.1674-1366.2017.02.005

Abstract

Abstract:

Objective

To explore the methods and surface characteristics of bone morphogenetic protein 2 (BMP-2) immobilizcd on titanium (Ti) surface by electrostatic interaction and to offer a new biochemical surface modification technology of Ti surface.

Methods

Pure Ti foils (10 mm in diameter and 0.3 mm in thickness) were randomly divided into 3 groups for the following processing: polished Ti surface (blank control) ; negative polymer on Ti surface after coating gold nanoparticles by magnetron sputtering and connecting polymer BPP (negative control, Ti/BPP) ; the immobilization of BMP-2 protein by electrostatic adsorption and chemical reaction on Ti/BPP (experimental group, Ti/BPP/BMP-2) . Surface morphology, static water contact angle, surface roughness and Zeta potential were observed, respectively. The connection and distribution of BMP-2 was confirmed by fluorescence labeling, and the differences of the fluorescence intensity before and after the linking of fluorescein isothiocyanate (FITC) -BMP-2 on Ti/BPP were tested by fluorescence spectrophotometer. The integration rate of BMP-2 on Ti and Ti/BPP was measured with enzyme linked immunosorbent assay (Elisa) . The data analysis was conducted using One-Way ANOVA and independent sample T-test, while Bonferroni test was used for a multiple comparison procedure.

Results

The results of SEM and fluorescence labeling showed that BMP-2 was successfully grafted on Ti surfaces. The fluorescence intensity of FITC-BMP-2 decreased from 124.000 ± 0.968 to 84.060 ± 0.495 after the reaction with Ti/BPP (F= 37.727, P<0.001) . The surface zeta potential of Ti/BPP was -31.2 mV, while Ti/BPP/BMP-2 was +9.3 mV, when that of Ti was 0 mV. The integration rate of BMP-2 on Ti/BPP (14.883 ± 0.916) %, was significantly higher than that on Ti [ (5.990 ± 0.306)%; F= 6.000, P<0.001]. The hydrophilic property of Ti/BPP and Ti/BPP/BMP-2 group was much higher than that of Ti group, while there was no significant difference in the surface roughness of the three groups.

Conclusion

BMP-2 can be successfully grafted on Titanium surface by electrostatic adsorption and chemical reaction using negative polymer BPP.

Key words: Titanium, Bone morphogenetic protein 2, Nanoparticles, gold, Static electricity

Guorong Dai, Xinchun Zhang, Wenqing Wan, Yan Wang. The study of bone morphogenetic protein 2 immobilization on titanium surface with polymer electrostatic interaction[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2017, 11(02): 93-99.

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Figures/Tables 12

References 15

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组别	试件数	水接触角
Ti组	5	67.2 ± 0.4^a
Ti/BPP组	5	32.2 ± 1.1^b
Ti/BPP/BMP-2组	5	28.8 ± 1.1^b
F值	-	307.753
P值	-	<0.001

组别	试件数	水接触角
Ti组	5	67.2 ± 0.4^a
Ti/BPP组	5	32.2 ± 1.1^b
Ti/BPP/BMP-2组	5	28.8 ± 1.1^b
F值	-	307.753
P值	-	<0.001

组别	试件数	Ra	Rp
Ti组	5	0.377 ± 0.023	3.3 ± 0.5
Ti/BPP组	5	0.358 ± 0.024	3.0 ± 0.4
Ti/BPP/BMP-2组	5	0.384 ± 0.031	3.7 ± 0.4
F值	-	0.829	0.269
P值	-	0.481	0.773

组别	试件数	Ra	Rp
Ti组	5	0.377 ± 0.023	3.3 ± 0.5
Ti/BPP组	5	0.358 ± 0.024	3.0 ± 0.4
Ti/BPP/BMP-2组	5	0.384 ± 0.031	3.7 ± 0.4
F值	-	0.829	0.269
P值	-	0.481	0.773

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