The inhibition of miR-181a on proliferation of salivary adenoid cystic carcinoma

doi:10.3877/cma.j.issn.1674-1366.2015.06.004

Abstract

Abstract:

Objective

To investigate the effect of microRNA (miR) -181a in proliferation of salivary adenoid cystic carcinoma (SACC) in vitro and in vivo.

Methods

The SACC cell lines SACC-LM and SACC-83 were transfected with miR-181a mimics and miR-181a LNA respectively. QRT-PCR analysis was used to detect the expression of miR-181a in the paired SACC cell lines after transfection. MTT assay was performed to analyze the effect of miR-181a on proliferation after transfection. Western blot analysis was used to demonstrate the proliferation related genes after transfection. Tumorigenesis model in nude mice was performed to analyze the effect of miR-181a on proliferation of SACC cell lines after transfection in vivo. Data were analyzed using the Statistical Package for the Social Science (SPSS) , Version 17.0. For all statistical analyses, P<0.05 was considered statistically significant.

Results

Ectopic transfection of the miR-181a mimics to the SACC-LM cells led to increased miR-181a expression (t = -9.198, P = 0.012) while the miR-181a expression was decreased in SACC-83 cells treated with miR-181a LNA (t = -7.241, P = 0.019) . After transfection, the proliferation rate of SACC-LM cells was reduced (t = -4.58, P = 0.045) , while it was increased in SACC-83 cells (t = 3.016, P = 0.03) . Gene expression of TGF-β2, NGF and VEGF was decreased in SACC-LM cells after transfection, while increased in transfected SACC-83 cells. Ectopic transfection of the miR-181a mimics to the SACC-LM cells led to decrease in tumorigenesis ability (t = -4.692, P = 0.043) .

Conclusion

MiR-181a can suppress the proliferation of SACC in vitro and in vivo.

Key words: MicroRNA-181a, Salivary glands, Carcinoma, adenoid cystic, Proliferation, In vitro, In vivo

Qianting He, Dan Chen, Tingting Zhao, Zhonghua Liu, Anxun Wang. The inhibition of miR-181a on proliferation of salivary adenoid cystic carcinoma[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(06): 455-460.

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mircroRNA	引物	引物序列
Hsa-miR-181a	HmiR-181a-RT	GTCGGGTCCAGAGCAGGGTCCGAGGTA
?	?	CACGTTCGCTCTGGACCCGACACTCAC
?	HmiR-181a-FO-1	TGCCGAACATTCAACGCT
?	HmiR-RE-4	AGAGCAGGGTCCGAGGTA
U6snRNA	U6snRNA FO	ATTGGAACGATACAGAGAAGATT
?	U6snRNA RE	GGAACGCTTCACGAATTTG

mircroRNA	引物	引物序列
Hsa-miR-181a	HmiR-181a-RT	GTCGGGTCCAGAGCAGGGTCCGAGGTA
?	?	CACGTTCGCTCTGGACCCGACACTCAC
?	HmiR-181a-FO-1	TGCCGAACATTCAACGCT
?	HmiR-RE-4	AGAGCAGGGTCCGAGGTA
U6snRNA	U6snRNA FO	ATTGGAACGATACAGAGAAGATT
?	U6snRNA RE	GGAACGCTTCACGAATTTG

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