In vitro study on the effect of ultraviolet irradiation on induction apatite formation and cell mineralization of micro-arc oxidized titanium surface

doi:10.3877/cma.j.issn.1674-1366.2015.05.005

Abstract

Abstract:

Objective

To evaluate the effect of ultraviolet irradiation on induction apatite formation and cell mineralization of micro-arc oxidized titanium surfaces, using cell culture medium immersion tests and interactions between adipose derived mesenchymal stem cells and material surfaces.

Methods

Pure titanium disks were treated by micro-arc oxidation (MAO) , as blank control group, marked as MAO. MAO combined with ultraviolet irradiation treatment for 2 hours, as treatment group, marked as MAO + UV 2 h. Apatite formation on the surface of titanium disks and the cell mineralization were observed by a scanning electron microscopy (SEM) after immersed in DMEM/F12 medium and cultured with rat adipose derived mesenchymal stem cells (AMSCs) .

Results

Immersed in medium for 21 days, few scattered tiny apatite crystal particles were found in MAO group; while in MAO + UV 2 h group, increasing size and laminated deposition of apatite crystal particles could be observed. Almost the whole oxide layer was covered by them, and some deposited in the aperture of the titanium surface. After AMSCs cultured, no mineral compound formation was found in MAO group; however, in MAO + UV 2 h group, lots of needle crystals formed and EDS result suggested them as apatite crystals.

Conclusion

In vitro ultraviolet irradiation can promote the abilities of induction apatite formation and cell mineralization of micro-arc oxidized titanium surfaces.

Key words: Ultraviolet irradiation, Micro-arc oxidation, Apatites, Adipose tissue, Mesenchymal stem cells

Zhipeng Li, Runheng Liu, Zhuofan Chen, Baoxin Huang, Xiayi Wu, Xin Luo, Mingxuan Hu. In vitro study on the effect of ultraviolet irradiation on induction apatite formation and cell mineralization of micro-arc oxidized titanium surface[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(05): 379-384.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2015.05.005

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