A preliminary study on effects of hypoxia on the proliferation, cell cycle and differentiation of human periodontal ligament cells

doi:10.3877/cma.j.issn.1674-1366.2015.03.001

Abstract

Abstract:

Objective

To investigate the biological effect of hypoxia on the proliferation, cell cycle and expression level of stem cell marker (STRO-1 and CD146) of human periodontal ligament cells (hPDLCs) .

Methods

HPDLCs were isolated and cultured by the method of tissue lump. Third to fifth passage of HPDLCs were cultured at the condition of hypoxia (1.5%~2.0% O₂) and normaxia for 12, 24, 36 and 48 h, and MTT was used to evaluate the proliferation of HPDLCs; cell cycle and expression level of stem cell marker STRO-1 and CD146 was detected by flow cytometry.

Results

Both of hypoxia and normoxia groups of HPDLCs proliferation gradually increased as time went on. Proliferation rate of HPDLCs was slight higher in the hypoxia groups than in the normoxia groups at 12 or 24 h, and both were not significantly statistical difference. At 36 h or 48 h of incubation, proliferation rate of HPDLCs in hypoxia groups were lower than in normoxia groups (t_{36 h} = 3.538, P_{36 h} = 0.024; t_{48 h} = 5.349, P_{48 h} = 0.006) ; Proliferative index (PI) of HPDLCs in hypoxia group was slightly lower than in normoxia group at 12 h of incubation; PI of HPDLCs in hypoxia group was slightly higher than in normoxia group at 24 h or 36 h of incubation, but both were not statistically difference. PI of hPDLCs in hypoxia group was significantly higher than in normoxia group at 48 h of incubation (t_{48 h}=-5.768, P_{48 h}= 0.004) . The expression level of STRO-1 and CD146 were not significantly statistical difference in both of hypoxia group and normoxia group at four tested time point.

Conclusions

Hypoxia inhibits the proliferation of hPDLCs, but promote the DNA synthesis of hPDLCs. HPDLCs can maintain differentiation potential under hypoxic conditions.

Key words: Cell hypoxia, Human periodontal ligament cells, Cell proliferation, Cell cycle, Differentiation potential

Han Zou, Yunsheng Wen, Zhiling Wu, Huanyou Liang, Jian Wu, Qian Tang. A preliminary study on effects of hypoxia on the proliferation, cell cycle and differentiation of human periodontal ligament cells[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(03): 177-184.

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Figures/Tables 11

References 38

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组别	组数	12 h	24 h	36 h	48 h
常氧组	5	0.415 ± 0.081	0.514 ± 0.116	0.727 ± 0.155	0.887 ± 0.120
低氧组	5	0.424 ± 0.082	0.515 ± 0.113	0.655 ± 0.123	0.732 ± 0.138

组别	组数	12 h	24 h	36 h	48 h
常氧组	5	0.415 ± 0.081	0.514 ± 0.116	0.727 ± 0.155	0.887 ± 0.120
低氧组	5	0.424 ± 0.082	0.515 ± 0.113	0.655 ± 0.123	0.732 ± 0.138

组别	组数	12 h	24 h	36 h	48 h
常氧组	5	0.300 ± 0.150	0.419 ± 0.070	0.307 ± 0.071	0.192 ± 0.036
低氧组	5	0.221 ± 0.182	0.519 ± 0.163	0.349 ± 0.076	0.368 ± 0.078

组别	组数	12 h	24 h	36 h	48 h
常氧组	5	0.300 ± 0.150	0.419 ± 0.070	0.307 ± 0.071	0.192 ± 0.036
低氧组	5	0.221 ± 0.182	0.519 ± 0.163	0.349 ± 0.076	0.368 ± 0.078

组别	组数	12 h	24 h	36 h	48 h
常氧组	4	52.42 ± 2.0	52.39 ± 1.2	52.27 ± 0.9	52.70 ± 1.8
低氧组	4	52.21 ± 2.6	52.37 ± 2.7	52.52 ± 1.6	52.81 ± 2.3

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