Effects of different-intensity constant magnetic fields on the proliferation and function of osteoblasts in mice

doi:10.3877/cma.j.issn.1674-1366.2015.03.002

Abstract

Abstract:

Objective

To investigate the effects of different-intensity constant magnetic fields (CMFs) on the proliferation and function of osteoblasts in mice.

Methods

The mice osteoblast cell line MC3T3-E1 were selected to culture, then stimulated by the CMFs with 0, 50, 100, 150 or 200 mT, and screened the intensity which promote and inhibit the growth of osteoblasts the most significantly (150, 200 mT) . MC3T3-E1 were divided into three group, and then stimulated by the CMFs with 0, 150, 200 mT, respectively. The proliferation rate of osteoblasts, PICP expression, ALP activity, OCN activity were evaluated.

Result

The CMFs with 150 mT can promote the mice osteoblast proliferation and function, while the CMFs with 200 mT can inhibit the mice osteoblast proliferation and function.

Conclusion

The CMFs can promote the mice osteoblast proliferation and function, and has 'intensity window’ effect. The CMFs beyond the certain level showed inhibitory effect, and thus 150 mT may be one of the possible 'intensity window’.

Key words: Constant magnetic fields, Osteoblasts, PICP, Alkaline phosphatase, Osteocalcin

Yang Zhou, Rongsheng Zeng, Chenyu Guan, Shida Hou. Effects of different-intensity constant magnetic fields on the proliferation and function of osteoblasts in mice[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(03): 185-192.

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Figures/Tables 12

References 28

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组别	孔数	第1次加载后	第2次加载后	第3次加载后
0 mT组	4	3.0900 ± 0.3310	4.3700 ± 0.1966	6.5150 ± 0.6247
50 mT组	4	3.3250 ± 0.2774	5.5725 ± 1.5862	8.8325 ± 1.2487^a
100 mT组	4	4.2675 ± 2.0092	11.1125 ± 2.7293^a	13.3275 ± 3.6167^a
150 mT组	4	5.3725 ± 0.5857^a	13.9375 ± 1.1000^a	16.6350 ± 3.8166^a
200 mT组	4	2.0125 ± 0.6502^a	2.1675 ± 0.8272^a	3.2425 ± 0.2865^a
300 mT组	4	2.1725 ± 0.7190^a	2.1285 ± 1.2401^a	2.9375 ± 0.8141^a

组别	孔数	第1次加载后	第2次加载后	第3次加载后
0 mT组	4	3.0900 ± 0.3310	4.3700 ± 0.1966	6.5150 ± 0.6247
50 mT组	4	3.3250 ± 0.2774	5.5725 ± 1.5862	8.8325 ± 1.2487^a
100 mT组	4	4.2675 ± 2.0092	11.1125 ± 2.7293^a	13.3275 ± 3.6167^a
150 mT组	4	5.3725 ± 0.5857^a	13.9375 ± 1.1000^a	16.6350 ± 3.8166^a
200 mT组	4	2.0125 ± 0.6502^a	2.1675 ± 0.8272^a	3.2425 ± 0.2865^a
300 mT组	4	2.1725 ± 0.7190^a	2.1285 ± 1.2401^a	2.9375 ± 0.8141^a

组别	孔数	第1次加载后	第2次加载后	第3次加载后	第4次加载后	第5次加载后	第6次加载后	第7次加载后
0 mT组	8	0.4426 ± 0.0509	0.5048 ± 0.0412	0.5542 ± 0.0716	0.5048 ± 0.0672	0.4096 ± 0.0577	0.3912 ± 0.0448	0.3708 ± 0.1530
150 mT组	8	0.6154 ± 0.1999^a	0.7478 ± 0.1233^a	0.8758 ± 0.0434^a	0.8989 ± 0.0737^a	0.7023 ± 0.0296^a	0.4498 ± 0.0578^a	0.4026 ± 0.0539
200 mT组	8	0.3145 ± 0.0858^a	0.4090 ± 0.0804^a	0.3900 ± 0.0338^a	0.3798 ± 0.1336^a	0.3707 ± 0.1004^a	0.3557 ± 0.1352^a	0.3407 ± 0.1517

组别	孔数	第1次加载后	第2次加载后	第3次加载后	第4次加载后	第5次加载后	第6次加载后	第7次加载后
0 mT组	8	0.4426 ± 0.0509	0.5048 ± 0.0412	0.5542 ± 0.0716	0.5048 ± 0.0672	0.4096 ± 0.0577	0.3912 ± 0.0448	0.3708 ± 0.1530
150 mT组	8	0.6154 ± 0.1999^a	0.7478 ± 0.1233^a	0.8758 ± 0.0434^a	0.8989 ± 0.0737^a	0.7023 ± 0.0296^a	0.4498 ± 0.0578^a	0.4026 ± 0.0539
200 mT组	8	0.3145 ± 0.0858^a	0.4090 ± 0.0804^a	0.3900 ± 0.0338^a	0.3798 ± 0.1336^a	0.3707 ± 0.1004^a	0.3557 ± 0.1352^a	0.3407 ± 0.1517

组别	孔数	第1次加载后	第2次加载后	第3次加载后	第4次加载后	第5次加载后	第6次加载后	第7次加载后
0 mT组	4	0.8215 ± 0.0688	1.1149 ± 0.0784	1.1548 ± 0.0907	1.0713 ± 0.1188	0.9477 ± 0.0549	0.9304 ± 0.0420	0.9232 ± 0.0711
150 mT组	4	0.8998 ± 0.0511	1.2541 ± 0.0565^a	1.3807 ± 0.0939^a	1.3798 ± 0.0985^b	1.2140 ± 0.1024^a	1.1188 ± 0.1251^a	1.0837 ± 0.1050^a
200 mT组	4	0.8644 ± 0.0193	0.8599 ± 0.0931^b	0.8343 ± 0.0639^b	0.8293 ± 0.0619^a	0.7927 ± 0.0745^a	0.7859 ± 0.0816^a	0.7728 ± 0.0682^a

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