Abstract:

Objective

To investigate the role of MTUS1/ATIP1 in suppression proliferation and induce apoptosis of oral tongue squmous cell carcinoma.

Methods

Investigate the expression levels of MTUS1 in human tongue cell carcinoma cell lines （UM1， SCC-15， SCC-9， Tca8113）. Tongue squamous cancer cell were transfected with a ATIP1 expression vector for 48 h. The Proliferation analysis was performed with MTT assay. Flow cytometry and immunofluorescence technique were employed to measure cell cycle and apoptosis of tongue cell carcinoma cells. The expression levels of proteins， MTUS1， p53，ERK1/2， in tongue cell carcinoma cells transfected with ATIP1 were detected by Western blotting.

Results

In vitro functional study showed that over-expression of MTUS1/ATIP1 significantly increased the apoptosis rate （P＜0.05）， led to G1 arrest and decreased the proliferation activity， with an inhibition rate of about 40% （P＜0.05） in OTSCC cell lines. The over-expression of MTUS1/ATIP1 induced the reduction of phosphor-ylation of ERK and up-regulation of p53 and ERK.

Conclusion

MTUS1/ATIP1 is suppose to be associated with inhibit proliferation and induce apoptosis in oral tongue squmous cell carcinoma.

Key words: Tongue squmous cell carcinoma, MTUS1/ATIP1, Proliferation, Apoptosis