Analysis of ability of extracellular polysaccharide synthesis by Enterococcus faecalis biofilm-grown cells in starvation state

doi:10.3877/cma.j.issn.1674-1366.2012.05.005

Abstract

Abstract:

Objective

To investigate the influence of starvation on the ability of extracellular polysaccharide synthesis by Enterococcus faecalis （E.faecalis） biofilm-grown cells.

Methods

E.faecalis in starvation phase was developed to form biofilm. Fluorescent stains and inverted fluorescence microscope were used to monitor the distribution of extracellular polysaccharide in biofilm. Anthrone method was used to quantify the amount of extracellular polysaccharide in planktonic E.faecalis and the biofilms developed by E.faecalis cells in exponential， stationary and starvation phases.

Results

Extracellular polysaccharide was observed in 48 h biofilm formed by E.faecalis cells in starvation phase. The water soluble and water insoluble polysaccharide in the biofilm were significantly higher than those in planktonic cells （P＜0.05）. The differences of the amount of water soluble polysaccharide among the biofilms developed by cells in different physiologic states were not significant （P＞0.05）. The water insoluble polysaccharide in the biofilm formed by starved cells was higher than those formed by cells in exponential and stationary phases （P＜0.05）.

Conclusion

The ability of extracellular polysaccharide synthesis by E.faecalis biofilm-grown cells increased significantly in nutrient-poor environment.

Key words: Enterococcus faecalis, Biofilm, Extracellular polysaccharide, Starvation

Hong-yan LIU, Xi WEI, Jun-qi LING. Analysis of ability of extracellular polysaccharide synthesis by Enterococcus faecalis biofilm-grown cells in starvation state[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 6(05): 416-420.

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Figures/Tables 3

References 23

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组别	水溶性胞外多糖	水不溶性胞外多糖
浮游细菌	0.98±0.38	17.13±0.01
生物膜细菌	1.92±0.83^a	34.01±2.11^a

组别	水溶性胞外多糖	水不溶性胞外多糖
浮游细菌	0.98±0.38	17.13±0.01
生物膜细菌	1.92±0.83^a	34.01±2.11^a

组别	水溶性胞外多糖	水不溶性胞外多糖	胞外多糖总量
对数期48 h生物膜	1.53±0.32	3.12±0.33^a	4.66±0.32^a
稳定期48 h生物膜	1.61±0.25	4.04±0.52^a	5.90±0.46^a
饥饿状态48 h生物膜	1.92±0.83	34.01±2.11	36.21±1.58

组别	水溶性胞外多糖	水不溶性胞外多糖	胞外多糖总量
对数期48 h生物膜	1.53±0.32	3.12±0.33^a	4.66±0.32^a
稳定期48 h生物膜	1.61±0.25	4.04±0.52^a	5.90±0.46^a
饥饿状态48 h生物膜	1.92±0.83	34.01±2.11	36.21±1.58

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