A study of tissue-engineered compound of dog bone marrow stromal cells and the novel porous scaffold in vitro

doi:10.3877/cma.j.issn.1674-1366.2011.04.005

Abstract

Abstract:

Objective

To investigate the biological effects of the cultured dog bone marrow stromal cells （BMSCs） with the novel porous scaffold poly（3-hydroxybutyrate-co-3-hydroxyvalerate）/sol gel bioactive glass （PHBV/SGBG）.

Methods

Dog BMSCs were cultured on the PHBV/SGBG scaffolds in vitro. The cell attachment and distribution on the scaffold were observed under a scanning electron microscope （SEM）. MTT assay and alkaline phosphatase（ALP） activity measurement were used to detect the effects of the PHBV/SGBG scaffolds on cell proliferation and differentiation.

Results

Dog BMSCs firmly attached to and grew well on the surface of PHBV/SGBG scaffolds. Obvious cell processes stretched into the micro-pores structure.After 3-day- and 6-day-culture with the PHBV/SGBG scaffolds， the proliferation and differentiation of dog BMSCs were improved.

Conclusion

It suggested that the compound of dog BMSCs and PHBV/SGBG scaffold with good biological properties would be a potential method for periodontal tissue engineering.

Key words: Bone marrow stromal cells, Scaffold, Compound, Periodontal tissue engineering

Qian TANG, Jian-hong CHEN, Nan-nan HUANG, Huan-you LIANG. A study of tissue-engineered compound of dog bone marrow stromal cells and the novel porous scaffold in vitro[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2011, 5(04): 367-373.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2011.04.005

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2011/V5/I04/367

Figures/Tables 8

References 15

1	El-Ghannam A. Bone reconstruction： from bioceramics to tissue engineering. Expert Rev Med Devices， 2005，2（1）：87-101.
2	Yarlagadda PK， Chandrasekharan M， Shyan JY. Recent advances and current developments in tissue scaffolding. Biomed Mater Eng，2005，15（3）：159-177.
3	陈建洪，唐倩，田词，等. 新型纳米仿生骨植入材料的生物相容性的初步研究. 中山大学学报（医学科学版）， 2008，29（5）：629-632.
4	唐倩，陈建洪，黄南楠，等. 新型复合骨植入材料的体内成骨性能研究［J/CD］. 中华口腔医学研究杂志：电子版， 2010，4（3）：232-238.
5	唐倩，陈建洪，吴坚，等. 多孔复合材PHBV/SGBG 的体外细胞相容性和体内成骨性. 中华生物医学工程杂志， 2010，16（1）：52-57.
6	Arinzeh TL， Peter SJ， Archambault MP， et al. Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect. J Bone Joint Surg Am， 2003，85-A（10）：1927-1935.
7	Pelled G， G T， Aslan H， et al. Mesenchymal stem cells for bone gene therapy and tissue engineering. Curr Pharm Des， 2002，8（21）：1917-1928.
8	Griffith LG， Naughton G. Tissue engineering-current challenges and expanding opportunities. Science， 2002，295（5557）：1009-1014.
9	Huang GT， Gronthos S， Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources： their biology and role in regenerative medicine. J Dent Res， 2009，88（9）：792-806.
10	Liu Y， Zheng Y， Ding G， et al. Periodontal ligament stem cell-mediated treatment for periodontitis in miniature swine. Stem Cells， 2008，26（4）：1065-1073.
11	Ouyang HW， Cao T， Zou XH， et al. Mesenchymal stem cell sheets revitalize nonviable dense grafts： implications for repair of large-bone and tendon defects. Transplantation， 2006，82（2）：170-174.
12	Kawaguchi H， Hirachi A， Hasegawa N， et al. Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells. J Periodontol， 2004，75（9）：1281-1287.
13	Kramer PR， Nares S， Kramer SF， et al. Mesenchymal stem cells acquire characteristics of cells in the periodontal ligament in vitro. J Dent Res， 2004，83（1）：27-34.
14	Rosengren A， Faxius L， Tanaka N， et al. Comparison of implantation and cytotoxicity testing for initially toxic biomaterials. J Biomed Mater Res A， 2005，75（1）：115-122.
15	Fukayama S， Tashjian AH Jr. Involvement of alkaline phosphatase in the modulation of receptor signaling in osteoblasts： evidence for a difference between human parathyroid hormone-related protein and human parathyroid hormone. J Cell Physiol， 1994，158（3）：391-397.

时间（d）	n	对照组	PHBV / SGBG 组	P
1	5	0.297 ± 0.005	0.299 ± 0.008	0.312
3	5	0.405 ± 0.006	0.456 ± 0.013	0.001
6	5	0.801 ± 0.010	0.873 ± 0.005	0.000

时间（d）	n	对照组	PHBV / SGBG 组	P
1	5	0.297 ± 0.005	0.299 ± 0.008	0.312
3	5	0.405 ± 0.006	0.456 ± 0.013	0.001
6	5	0.801 ± 0.010	0.873 ± 0.005	0.000

时间（d）	n	对照组	PHBV / SGBG 组	P
1	5	0.665 ± 0.068	0.677 ± 0.025	0.136
3	5	3.436 ± 0.128	4.405 ± 0.196	0.000
6	5	4.635 ± 0.205	6.621 ± 0.235	0.000

时间（d）	n	对照组	PHBV / SGBG 组	P
1	5	0.665 ± 0.068	0.677 ± 0.025	0.136
3	5	3.436 ± 0.128	4.405 ± 0.196	0.000
6	5	4.635 ± 0.205	6.621 ± 0.235	0.000

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