Relationship between secreted aspartyl proteinase of Candida albicans and severe early childhood caries

doi:10.3877/cma.j.issn.1674-1366.2010.05.005

Abstract

Abstract:

Objective

To investigate the relationship between secreted aspartyl proteinase（SAP） of Candida albicans and severe early childhood caries （S-ECC）.

Methods

The activities of the SAPs from 40 strains of Candida albicans isolated from the oral cavity of children with SECC and without caries were evaluated contrastively by YNB-BSA agar plate method and BSA as substrate with MTT method.In addition， the association between the activities of SAPs and the genotypes of Candida albicans by 25S rDNA-PCR was analyzed.

Results

All strains secrete SAPs and the activities of the S-ECC group （0.36±0.03， 1.59±0.92） were significantly higher than that of the caries-free group （0.39±0.05， 0.79±0.26； P=0.013， P ＜0.001）.The genotypes of Candida albicans were divided into groups A， B and C and the genotype B existed only in SECC group.The activities among genotypes A， B and C in S-ECC group were not statistically significant （P=0.323， P=0.492）.

Conclusions

The SAPs of Candida albicans at higher level were closely associated with the progress of S-ECC.However， there was not a significant association between the activities of SAPs and the distribution of genotypes of Candida albicans strains.

Key words: Candida albicans, Secreted aspartyl proteinase, Early childhood caries

Shuo GAO, Wei ZHAO, Rong-min QIU, Yan LIN. Relationship between secreted aspartyl proteinase of Candida albicans and severe early childhood caries[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2010, 4(05): 456-461.

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Figures/Tables 4

References 14

1	Costa CR， Passos XS， e Souza LK， et al.Differences in exoenzyme production and adherence ability of Candida spp.isolates from catheter，blood and oral cavity.Rev Inst Med Trop Sao Paulo， 2010，52（3）：139-143.
2	Klinke HT，Pnöisch R，Kriegel TM，et al.Immunohistochemical detection of the collagenolytic Candia albicans Sap2 proteinase in caries lesions.Caries Res， 2007，53：287.
3	Barros LM， Boriollo MF， Alves AC， et al.Genetic diversity and exoenzyme activities of Candida albicans and Candida dubliniensis isolated from the oral cavity of Brazilian periodontal patients.Arch Oral Biol， 2008，53（12）：1172-1178.
4	Mendes A， Mores AU， Carvalho AP， et al.Candida albicans biofilms produce more secreted aspartyl protease than the planktonic cells.Biol Pharm Bull， 2007，30（9）：1813-1815.
5	McCullough MJ， Clemons KV， Stevens DA.Molecular and phenotypic characterization of genotypic Candida albicans subgroups and comparison with Candida dubliniensis and Candida stellatoidea.J Clin Microbiol， 1999，37（2）：417-421.
6	de Carvalho FG， Silva DS， Hebling J， et al.Presence of mutans streptococci and Candida spp.in dental plaque/dentine of carious teeth and early childhood caries.Arch Oral Biol， 2006，51（11）：1024-1028.
7	Marchant S， Brailsford SR， Twomey AC， et al.The predominant microflora of nursing caries lesions.Caries Res， 2001，35（6）：397-406.
8	Rozkiewicz D， Daniluk T， Zaremba ML， et al.Oral Candida albicans carriage in healthy preschool and school children.Adv Med Sci，2006，51 Suppl 1：187-190.
9	Klinke T， Kneist S， de Soet JJ， et al.Acid production by oral strains of Candida albicans and lactobacilli.Caries Res， 2009，43（2）：83-91.
10	Kukletová M， Ruzicka F， Sedlácek I， et al.Isolation of Candida Spp.in Dental Plaque of ECC Affected Children.Int Poster J Dent Oral Med， 2008，10（2）：398-398.
11	Villar CC， Kashleva H， Nobile CJ， et al.Mucosal tissue invasion by Candida albicans is associated with E-cadherin degradation， mediated by transcription factor Rim101p and protease Sap5p.Infect Immun， 2007，75（5）：2126-2135.
12	Schaller M， Korting HC， Borelli C， et al.Candida albicans-secreted aspartic proteinases modify the epithelial cytokine response in an in vitro model of vaginal candidiasis.Infect Immun， 2005，73（5）：2758-2765.
13	Tamura M， Watanabe K， Mikami Y， et al.Molecular characterization of new clinical isolates of Candida albicans and C.dubliniensis in Japan：analysis reveals a new genotype of C.albicans with group I intron.J Clin Microbiol， 2001，39（12）：4309-4315.
14	Liu XP， Fan SR， Bai FY， et al.Antifungal susceptibility and genotypes of Candida albicans strains from patients with vulvovaginal candidiasis.Mycoses， 2009，52（1）：24-28.

组别	样本数	Pa	SAP / MTT
S-ECC 组	26	0.36 ± 0.03	1.59 ± 0.92
无龋组	14	0.39 ± 0.05	0.79 ± 0.26
P		0.013	＜ 0.001

组别	样本数	Pa	SAP / MTT
S-ECC 组	26	0.36 ± 0.03	1.59 ± 0.92
无龋组	14	0.39 ± 0.05	0.79 ± 0.26
P		0.013	＜ 0.001

基因型	样本数	Pa	SAP / MTT
A	9（34.6%）	0.34 ± 0.03	1.33 ± 0.67
B	8（30.8%）	0.37 ± 0.02	1.74 ± 0.54
C	9（34.6%）	0.36 ± 0.04	1.73 ± 1.34
P		0.323	0.492

基因型	样本数	Pa	SAP / MTT
A	9（34.6%）	0.34 ± 0.03	1.33 ± 0.67
B	8（30.8%）	0.37 ± 0.02	1.74 ± 0.54
C	9（34.6%）	0.36 ± 0.04	1.73 ± 1.34
P		0.323	0.492

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