Differentially expressed genes of Candida albicans under starvation conditions

doi:10.3877/cma.j.issn.1674-1366.2016.02.003

Abstract

Abstract:

Objective

To explore the gene expression during induction and formation of starvation state of Candida albicans (C.albicans) .

Methods

C.albicans American Type Culture Collection (ATCC) 10231 was cultured and induced into starvation phase. After induction for 0, 12, 24, and 48 h, the fungal cells were collected. Total RNA was extracted and microarrays were used to identify the gene expression changes under starvation conditions. T-test was used to statistically analyze the data of different time points.

Results

Statistical analysis demonstrated that 14.45% to 29.13% of gene expression has changed during the induction into starvation conditions (P<0.05) , these genes are related with many biologic pathways including amino acid metabolism, carbohydrate metabolism, lipid metabolism, nucleic acid metabolism and cell cycles. Especially, the differentially expressed genes included the down-regulation of alanine metabolism related genes, ATP synthesis related gene SDH4, mitosis related gene PRE1, and exopolymeric matrix production gene GCA1, and up-regulation of PXA2 and VPS34.

Conclusions

During the induction into starvation conditions, the genes related with metabolic and proliferative activities were down-regulated, while the genes related with environment and drug resistance were up-regulated. These changes may be contributed to maintain the survival and pathogenic capabilities of C.albicans under starvation conditions.

Key words: Candida albicans, Starvation condition, Microarray

Yang Ning, Yu Du, Junqi Ling. Differentially expressed genes of Candida albicans under starvation conditions[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2016, 10(02): 92-96.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2016.02.003

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2016/V10/I02/92

Figures/Tables 1

References 20

[1]	Goldfein J, Speirs C, Finkelman M，et al. Rubber dam use during post placement influences the success of root canal-treated teeth[J]. J Endod，2013，39(12):1481-1484.
[2]	Liu H, Wei X, Ling J，et al. Biofilm formation capability of Enterococcus faecalis cells in starvation phase and its susceptibility to sodium hypochlorite[J]. J Endod，2010，36(4):630-635.
[3]	Siqueira JF Jr, Rôças IN. Diversity of endodontic microbiota revisited[J]. J Dent Res，2009，88(11):969-981.
[4]	Ning Y, Hu X, Ling J，et al. Candida albicans survival and biofilm formation under starvation conditions[J]. Int Endod J，2013，46(1):62-70.
[5]	Ogasawara A, Odahara K, Toume M，et al. Change in the respiration system of Candida albicans in the lag and log growth phase[J]. Biol Pharm Bull，2006，29(3):448-450.
[6]	Leach MD, Stead DA, Argo E，et al. Molecular and proteomic analyses highlight the importance of ubiquitination for the stress resistance，metabolic adaptation，morphogenetic regulation and virulence of Candida albicans[J]. Mol Microbiol，2011，79(6):1574-1593.
[7]	Watanabe T, Ogasawara A, Mikami T，et al. Hyphal formation of Candida albicans is controlled by electron transfer system[J]. Biochem Biophys Res Commun，2006，348(1):206-211.
[8]	Sturtevant J, Dixon F, Wadsworth E，et al. Identification and cloning of GCA1，a gene that encodes a cell surface glucoamylase from Candida albicans[J]. Med Mycol，1999，37(5):357-366.
[9]	Nobile CJ, Nett JE, Hernday AD，et al. Biofilm matrix regulation by Candida albicans Zap1[J]. PLoS Biol，2009，7(6):e1000133.
[10]	Singh P, Chauhan N, Ghosh A，et al. SKN7 of Candida albicans：mutant construction and phenotype analysis[J]. Infect Immun，2004，72(4):2390-2394.
[11]	Higgins CF. ABC transporters：physiology，structure and mechanism—an overview[J]. Res Microbiol，2001，152(3-4):205-210.
[12]	Higgins CF, Linton KJ. The ATP switch model for ABC transporters[J]. Nat Struct Mol Biol，2004，11(10):918-926.
[13]	St Georgiev V. Membrane transporters and antifungal drug resistance[J]. Curr Drug Targets，2000，1(3):261-284.
[14]	Chen LM, Xu YH, Zhou CL，et al. Overexpression of CDR1 and CDR2 genes plays an important role in fluconazole resistance in Candida albicans with G487T and T916C mutations[J]. J Int Med Res，2010，38(2):536-545.
[15]	Prasad R, Sharma M, Rawal MK. Functionally Relevant Residues of Cdr1p：A Multidrug ABC Transporter of Human Pathogenic Candida albicans[J]. J Amino Acids，2011(2011):531412.
[16]	Jezewski S, von der Heide M, Poltermann S，et al. Role of the Vps34p-interacting protein Ade5，7p in hyphal growth and virulence of Candida albicans[J]. Microbiology，2007，153(Pt7):2351-2362.
[17]	Günther J, Nguyen M, Härtl A，et al. Generation and functional in vivo characterization of a lipid kinase defective phosphatidylinositol 3-kinase Vps34p of Candida albicans[J]. Microbiology，2005，151(Pt1):81-89.
[18]	Kitanovic A, Nguyen M, Vogl G，et al. Phosphatidylinositol 3-kinase VPS34 of Candida albicans is involved in filamentous growth，secretion of aspartic proteases，and intracellular detoxification[J]. FEMS Yeast Res，2005，5(4-5):431-439.
[19]	Murciano C, Moyes DL, Runglall M，et al. Evaluation of the role of Candida albicans agglutinin-like sequence（Als）proteins in human oral epithelial cell interactions[J]. PLoS One，2012，7(3):e33362.
[20]	Dwivedi P, Thompson A, Xie Z，et al. Role of Bcr1-activated genes Hwp1 and Hyr1 in Candida albicans oral mucosal biofilms and neutrophil evasion[J]. PLoS One，2011，6(1):e16218.

Please choose a citation manager

Content to export